Quantitative detection method of polysaccharide containing uronic acid
A technology for quantitative detection of uronic acid polysaccharides, which is used in measurement devices, instruments, scientific instruments, etc., can solve the problem that the degrading enzymes of glycosaminoglycanase are expensive, the polysaccharide content cannot be determined, and the chemical color development method lacks specificity, etc. problem, to achieve the effect of simple operation, low cost and accurate results
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[0036] Step 1: Accurately take about 10 mg each of heparin, chondroitin sulfate, hyaluronic acid, and abalone gonadal polysaccharide AGSP. After mixing, add 5 ml of water, dissolve, overnight, and dilute to 10 ml volumetric flask with 2.6 mol / L trifluoroacetic acid. Shake well to make standard mother liquor. Then dilute it with 1.3mol / L trifluoroacetic acid, and prepare mixed standard polysaccharide solutions with serial concentrations (0.02, 0.04, 0.06, 0.08, 0.10, 0.20, 0.40, 0.6, 0.8, 1.0mg / ml), each concentration is two parallel.
[0037] Step 2, prepare the polysaccharide solution of the sample to be tested: take 100 mg of the contents of glucosamine chondroitin sulfate calcium capsules in a 25ml volumetric flask, dilute to volume with water, shake well, pass through a 0.22 μm filter membrane overnight, and obtain the polysaccharide solution of the sample to be tested; accurately measure Take 0.50ml of the polysaccharide solution of the sample to be tested in a 5ml hydro...
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