43 results about "Pazufloxacin" patented technology

The invention belongs to the field of medicine analysis, and particularly relates to a chiral stationary-phase detection method for the dextroisomer of pazufloxacin mesilate injection. The invention aims at providing a method which is simple and convenient to operate, and capable of rapidly and accurately detecting a dextroisomer, wherein HPLC (high performance liquid chromatography) detection conditions are as follows: 5mu m silica gel coated with amylose-tri(3,5-dimethylphenyl carbamate) on the surface or 5mu m silica gel coated with amylose-tri[(S)-alpha-methylphenyl carbamate] on the surface is used as a filler in a stationary phase; a mobile phase A is a phosphate buffered solution; a mobile phase B is acetonitrile or ethanol, wherein the phosphate buffered solution contains 10-100 mM of monopotassium phosphate; the pH value is adjusted to 1.5-4.0 by phosphoric acid; a mobile phase is prepared from the mobile phase A and the mobile phase B in a volume ratio of (50 to 90): (50 to 10); a flow speed is 1.0 ml / min; a column temperature is 10-25 DEG C; a detection wavelength is 240 nm; and the number of theoretical plates is not less than 2500 counted by the peaks of the laevoisomer of pazufloxacin, and a separation degree between the laevoisomer and the dextroisomer of pazufloxacin needs to meet requirements.

An anti-quinolone antibiotic class specific monoclonal antibody hybridoma cell strain YH6 and an application thereof belong to the technical field of immunochemistry. The monoclonal cell strain YH6 is preserved in China General Microbiological Culture Collection Center with the preservation number of CGMCC No.12024. A monoclonal antibody secreted by the YH6 is detected by indirect competitive enzyme-linked immunosorbent assay, and has cross reaction with the following 21 pyrethroids: norfloxacin, ofloxacin, enrofloxacin, ciprofloxacin, flumequine, nafloxacin, enoxacin, lomefloxacin, levofloxacin, pefloxacin, nalidixic acid, danofloxacin, pyridine acid, cinoxacin, oxolinic acid, marbofloxacin, pazufloxacin, sparfloxacin, gatifloxacin, orbifloxacin and fleroxacin, and the IC50 value of the monoclonal antibody is 0.1-50 ng / mL. The class specific monoclonal antibody can be used for developing colloidal gold immunochromatographic test strips and immunosensors, provides a raw material for the immunodetection of quinolone antibiotic residues in foods, and has practical application values.

The invention relates to a pazufloxacin mesylate tablet and a preparation method and a detection method thereof, which belong to the technical field of medicines. A medicinal oral tablet with high dissolvability and high stability is prepared by the following steps of: screening the formula components of the pazufloxacin mesylate tablet; and selecting suitable adhesives and adopting specific coating materials. Clinical trials show that the pazufloxacin mesylate tablet has an exact curative effect, a few side effects and high safety.

The invention provides a preparation method for pazufloxacin mesilate for injection. The preparation method comprises the following steps of: preparing the pazufloxacin mesilate and mannitol according to a prescription, and regulating pH values to be 3.0-4.0; adding a proper amount of needle active carbon, heating the mixture to be 70-80 DEG C, and carrying out decoloring after stirring for 15-20 minutes; putting an encapsulated semi-finished product into a freezing vacuum drier, controlling the temperature to be below -30 DEG C and carrying out prefreezing for 1-1.5 hours; when the temperature of a cold trap of the drier is lower than -40 DEG C, vacuumizing until the pressure is 20 Pa, and carrying out constant-temperature drying for 0.5-1 hour; raising the temperature of a separation board of the drier to be -10 DEG C, carrying out the constant-temperature drying for 0.5-1 hour; and raising the temperature of the separation board to be 0 DEG C, carrying out the constant-temperature drying until the waterline of the product reaches the bottom, raising the temperature to be 10 DEG C in a temperature manner, after carrying out the constant-temperature drying until the temperature of the product approximates to 0 DEG C, raising the temperature of the separation board to be 35 DEG C, and judging that the product is qualified after carrying out the constant-temperature drying until reaching the end point. The preparation method has the advantages that the production cost is lowered and the product quality is improved and is more suitable for industrial production.

The invention discloses a ethanesulfonic acid Pazufloxacin ad aurem gel auristilla, which take ethanesulfonic acid Pazufloxacin for the major components, and is produced complemented by Carbomer, glycerin, sodium hydroxide, propanediol and sterile water for injection or purified water or distilled water. The invention also discloses the preparation method of the said ethanesulfonic acid Pazufloxacin ad aurem gel auristilla. The invention of this medicine is a new generation quinolone synthesis antibacterial drugs, with high bioavailability, can reduce the dosage in clinical application, dosage interval extension, reduction the adverse reactions of systemic absorption, helping improving patients medication compliance. Meanwhile, the invention of the drug overcomes the resistance which the old varieties drugs bring to patients, solves the need of new medicines for the clinical treatment of ear infections diseases.

The invention discloses a method for detecting small polar impurities in pazufloxacin mesylate bulk drug, which adopts high performance liquid chromatography for detection, and the mobile phase is acetonitrile-10% triethylamine mesylate solution-1. 0mol / L dipotassium phosphate-water with the volume ratio of 45: 10: 7: 138; the method comprises the following steps: respectively taking a test solution and a contrast solution, injecting the test solution and the contrast solution into a high performance liquid chromatograph, and recording the retention time of a chromatogram to 7 times of a pazufloxacin mesylate characteristic peak. According to the present invention, the pazufloxacin mesylate bulk drug is detected by using the HPLC, and the small polar impurity with the retention time greater than the characteristic peak of the pazufloxacin mesylate can be effectively detected within the 7-time retention time of the pazufloxacin mesylate by using the specific ratio of the mobile phase; therefore, the quality control of the pazufloxacin mesilate bulk drug can be realized more accurately and scientifically.

An anti-quinolone antibiotic class specific monoclonal antibody hybridoma cell strain YH6 and an application thereof belong to the technical field of immunochemistry. The monoclonal cell strain YH6 is preserved in China General Microbiological Culture Collection Center with the preservation number of CGMCC No.12024. A monoclonal antibody secreted by the YH6 is detected by indirect competitive enzyme-linked immunosorbent assay, and has cross reaction with the following 21 pyrethroids: norfloxacin, ofloxacin, enrofloxacin, ciprofloxacin, flumequine, nafloxacin, enoxacin, lomefloxacin, levofloxacin, pefloxacin, nalidixic acid, danofloxacin, pyridine acid, cinoxacin, oxolinic acid, marbofloxacin, pazufloxacin, sparfloxacin, gatifloxacin, orbifloxacin and fleroxacin, and the IC50 value of the monoclonal antibody is 0.1-50 ng / mL. The class specific monoclonal antibody can be used for developing colloidal gold immunochromatographic test strips and immunosensors, provides a raw material for the immunodetection of quinolone antibiotic residues in foods, and has practical application values.

The invention discloses a preparation method and application of a pazufloxacin iridium complex. The pazufloxacin iridium complex at least contains one of the following chemical formulas: [Ir (ppy) 2 (mbpyPAZ)] PF6, [Ir (bzq) 2 (mbpyPAZ)] PF6; [Ir (dfppy) 2 (mbpyPAZ)] PF6; [Ir (thpy) 2 (mbpyPAZ)] PF6; [Ir (pq) 2 (mbpyPAZ)] PF6. The pazufloxacin iridium complex has antibacterial and anti-tumor dual functions, has certain selectivity, and has extremely low cytotoxicity to normal cells L-02.

The invention discloses a pazufloxacin mesilate xylitol injection which is characterized by containing the components as follows: 1g to 10g of pazufloxacin mesilate and 43g to 100g of xylitol, wherein methanesulfonic acid is used for regulating the PH value to 2.0 to 6.0, and water for injection is added to reach 1000ml. Furthermore, the invention further provides a preparation method of the pazufloxacin mesilate xylitol injection. Compared with the prior art, the pazufloxacin mesilate xylitol injection and the preparation method thereof have the advantage that: the isomer and relevant substances of the pazufloxacin mesilate xylitol injection under illumination and at high temperature are within the qualified scope by means of study and test on thermal stability, light stability and low temperature.