The invention discloses a hygienic disinfectant wet tissue and a preparation method thereof, and belongs to the technical field of wet tissues. The hygienic disinfectant wet tissue is prepared through the following steps: preparing a wetting solution by utilizing the following components in percentage by weight: 0.1-10% of wood seed extract, 0-5% of humectant, 0-5% of antibacterial agent, 0-5% of decontaminant, 0-5% of emollient, and the balance of purified water; and soaking non-woven fabric cloth or cotton cloth into the wetting solution, or spraying the wetting solution on the non-woven fabric cloth or cotton cloth so as to obtain the hygienic disinfectant wet tissue. According to the invention, because the wood seed extract contains the skin protection ingredient tannin, the extract acts as the ingredient of the wetting solution, has natural, non-toxic, environmentally-friendly performance characteristics and the like, is a favorable disinfectant appliance, and is especially suitable for rabo vermelho of babies; and the humectant, the antibacterial agent, the decontaminant, the emollient and the like are added into the wetting solution, and can generate effects rapidly for skin problems of dermatitis, eczema, bedsore, rabo vermelho of babies and the like, and the hygienic disinfectant wet tissue and the preparation method thereof also have the obvious effects of decontamination, anti-inflammation and pain relieving, bacteriostasis and itching prevention, and skin protection.