552 results about "Enzyme linked immunoassay" patented technology

The invention provides a method for realizing immunization detection for an anti-nucleosome antibody IgG (intravenous gamma globulin) based on the enzyme-linked immunoassay principle and a reagent device therefor; and an analytic method, the reagent device and an assorted reagent are independently, individually and disposably used for detecting the anti-nucleosome antibody IgG based on enzyme-linked immunoassay. Various reagents needed by enzyme-linked immunoassay for the anti-nucleosome antibody IgG are contained in one analytic device; and by using the method, the related immunology detection can be conveniently carried out according to the using requirements of detection items so as to provide better basis for clinical application.

The invention relates to the field of biomedicine, in particular to an enzyme-linked immunization diagnostic reagent kit for detecting an enterovirus (EV) 71-type antibody (immune globulin M (IgM)), and a preparation method and application of the diagnostic reagent kit. The probability of hand-foot-and-mouth disease and severe infection (viral encephalitis, viral cerebrospinal meningitis and pulmonary edema) caused by EV71 type is relatively higher, and case fatality rate is relatively higher and can be 10 to 25 percent. The enzyme-linked immunization diagnostic reagent kit of the EV71-IgM antibody can be used for diagnosing the infection of the EV71 type. According to related documents about the detection of the EV71-IgM, EV71 virus cultures serving as indirect enzyme-linked immuno sorbent assay (ELISA) of envelope antigens has defects in such aspects as specificity, sensitivity and stability, and due to high cultivation cost and low efficiency, a large amount of virus cannot be supplied to the market. In order to overcome the defects, the invention provides the reagent kit which is used for detecting the EV71-IgM in human blood serum, required by clinical examination, simple and convenient to operate and applicable to all medical disease control departments, and the preparation method and the application of the reagent kit. The invention has the technical scheme that: firstly, the human blood serum is added into a micro-pore plate, wherein the IgM antibody is obtained by an anti-mu chain which is pre-enveloped on the micro-pore plate, and other uncombined components are washed and removed; secondly, an enzyme labeling object is added, the EV71-IgM in the obtained IgM can be combined with the specificity of an EV71 recombinant antigen which is labeled by horse radish peroxidase (HRP), and after washing, the HRP can react with substrates which are added subsequently; and finally, the aim of detecting the EV71-IgM antibody is fulfilled.

The invention discloses a method for preparing polyclonal antibody against mouse nerve growth factor (NGF) and application thereof in an enzyme-linked immunoassay method. The polyclonal antibody which is prepared by using mouse submandibular gland NGF as an immunogen can be used for quantitatively detecting the mouse growth factor. Therefore, an assembled kit can determine fixed position and expression of different tissue cell nerve growth factors and detect NGF contents in different samples such as blood, cells, tissues, culture medium supernatant, and the like.

The invention discloses an enzyme-linked immunoassay kit of a structural protein antibody for a seneca valley virus. The kit comprises an elisa plate, positive control serum, negative control serum, an HRP-conjugated antibody, a sample diluent, a 20-fold concentrated detergent, a substrate solution A, a substrate solution B and a stop solution, wherein the elisa plate is coated with a structural protein epitope polypeptide composition for the seneca valley virus. The epitope polypeptide composition is one or any combination of more than two of a polypeptide as shown in a sequence 1, a polypeptide as shown in a sequence 2, a polypeptide as shown in a sequence 3 or a polypeptide as shown in a sequence 4 in the sequence table. The elisa plate is coated with a chemical synthetic antigen peptide, so that the kit is low in antigen dosage and high in sensitivity and specificity, and whether the structural protein antibody is infected by the seneca valley virus or not can be efficiently detected. The kit is high in sensitivity, good in specificity, convenient in operation, and has a good market prospect.

The invention discloses a method for quantitatively detectingan oleylamine grafted polysuccinimide(PSIOAm) macromolecule nanometer drug carrier based on an indirect competitive enzyme-linked immunosorbent assay. A secondary antibody is utilized, so that a detection signal is amplified, and the sensitivity of experimental analysis is improved; the aim of quantitatively detecting the PSIOAm is achieved through measuring an optical signal of a compound of a PSIOAm envelope antigen, a PSIOAm antibody which is the primary antibody and an HRP-marked goat anti-rabbit antibody which is the secondary antibody. The method is simple to operate, high in feasibility, high in sensitivity, low in detection limit, and capable of realizing high throughput detection.

The present invention discloses an enzyme-linked immunoassay kit for detecting phenylethanolamine A. The kit contains a specific antibody of the phenylethanolamine A, wherein the specific antibody is packaged separately. The specific antibody of the phenylethanolamine A is a polyclonal antibody or a monoclonal antibody prepared by adopting a conjugate of the phenylethanolamine A and a carrier protein as immunogen. According to the present invention, an ELISA competition method is adopted to qualitatively or quantitatively detect the residue level of the phenylethanolamine A drug in animal urine, serum, tissue (muscle, liver and kidney), feed, and other samples; the pre-treatment requirements on the sample are low; the animal urine and the animal serum can be detected directly; the tissue samples and the feed can be loaded on the kit after sample liquid extraction; a large number of samples can be concurrently and rapidly detected; the detecting method has characteristics of simpleness and time saving, and the result can be obtained within one hour; and the lowest detection limit of the pig urine sample is 0.5 mug / L. The kit of the present invention has characteristics of high specificity, high sensitivity, high precision and high accuracy, and plays important roles in detections of the phenylethanolamine A residue in animal products.