69results about How to "Achieve accurate quantification" patented technology

The invention discloses uPAR (urokinase-type plasminogen activator receptor) targeted peptides with the amino acid sequence indicated in SEQ ID NO. 1. The uPAR targeted peptides is linear peptides formed by 13 amino acids, the peptides are easy to synthesize and can form a stable conformation and be combined with uPARs with specific target. The invention discloses a uPAR targeted probe comprisinga single unit and the above uPAR targeted peptides. The uPAR targeted probe is subjected to specific identification of the peptides and is combined with the uPARs, signals for detection of imaging equipment are transmitted through the signal unit, living visual detection of the uPARs is achieved, and distribution and number of the uPARs are displayed visually and are used for disease detection foruPAR expression abnormalities. The invention further discloses a living molecular imaging method. The uPAR targeted probe is utilized, and the living molecular imaging method has the advantages of being safe, non-invasive, dynamic, visual and the like and is suitable for direct detection of human body.

The present invention relates to a metal material surface electroslag quick-equally heating and combination equipment. It is formed from equally-heating device, melting device and intercommunication device. It is characterized by that said intercommunication device is respectively connected with melting device and equally-heating device. Said invention is simple in structure, and can be used for producing various composite workpieces with several shapes.

The invention provides a noninvasive biopsy virus detection method based on high throughput gene sequencing. The method can carry out DNA sequencing and RNA sequence in parallel and thus precisely determines the biological composition and structure of viruses in patients. The method can maximally avoid the interference of nucleic acid of host cells, effectively reduce the cost of sequencing, and is capable of detecting multiple unknown crossed viruses at the same time. 80 DNA tagged connectors are designed, related reagents are assembled to form a library so as to construct a kit, and if the throughput allows, the kit can perform sequencing on 80 samples in parallel. The experiment design is strict, the automation degree is high, the virus identification can be finished in batches within 24 hours, the requirements of scientific research and clinical detection can be met in different levels, and references are provided for clinical diagnosis and prescription of diseases related with virus infection.

The invention relates to an in vitro detection method of an antibody. An antibody to be detected can be determined by using an antigen with more than two antibody recognition epitopes to simultaneously combine with the antibody to be detected and the antibody with a marker to directly detect the marker or detect the marker after adding a reaction substrate. The detection result is consistent with the cognition habit of people, the in vitro detection method is more convenient to operate and judge. With the method, the sensitivity and the repeatability of the detection are improved and the detection specificity is enhanced.

The invention discloses a method for detecting homopiperazine in fasudil hydrochloride, which uses gas chromatography to qualitatively or quantitatively detect the homopiperazine in the fasudil hydrochloride. The diluent used in the sampling detection solution in the gas chromatography comprises DBU and organic solvent. According to the method for detecting the homopiperazine in the fasudil hydrochloride, the homopiperazine in the fasudil hydrochloride can be detected effectively and veritably, and the quality and the safety of fasudil hydrochloride are guaranteed.

The invention relates to the field of detection, in particular to a method for quantitatively detecting melittin by liquid chromatography-tandem mass spectrometry, which comprises the step of detecting a characteristic peptide fragment of melittin in a sample to be detected by liquid chromatography-tandem mass spectrometry, wherein the sequence of the characteristic peptide fragment is VLTTGLPALISWIK. The method has high accuracy, precision and sensitivity, is high in stability, is suitable for accurate quantification of melittin in complex matrixes, and has important practical significance for protecting rights and interests of melittin consumers and maintaining healthy development of the bee product industry.

The invention discloses a system and a method for separating fluorine ions and low-molecular-weight organic acids by an ion-chromatographic-column switching method. The system comprises a sample injector, a six-way valve, a six-way valve switcher, an ion-exclusion column, a receiving pipe, a negative-ion exchange column, a liquid conveying pump, an electric-conductivity detector and a waste-liquid bottle, wherein the sample injector is connected with the six-way valve switcher which is connected with a liquid-phase pump and the ion-exclusion column; the six-way valve is connected with the receiving pipe, the liquid conveying pump and the negative-ion exchange column; and then the negative-ion exchange column is connected with a suppressor, the electric-conductivity detector and the waste-liquid bottle in sequence. The system and the method disclosed by the invention have the advantages that by use of the in-series connection of the conventional negative-ion exchange column and the ion-exclusion column, accurate quantification of the fluorine ions can be realized, the matrix interference in determination is reduced, online pretreatment of samples is realized and the artificial error and the manual labor are reduced; and the problem of interference of organic acids when conventional ion chromatography is used for determining the fluorine ions currently is solved and the independence of detection on the chromatographic column is reduced.

The invention provides a method for detecting carmustat mesylate related substances by adopting high performance liquid chromatography. A chromatographic column is an octadecylsilane chemically bondedsilica chromatographic column, and a mobile phase A is a mixed solution of sodium heptanesulfonate and trifluoroacetic acid; a mobile phase B is any one of acetonitrile, methanol, ethanol and tetrahydrofuran or a mixed solution of two of acetonitrile, methanol, ethanol and tetrahydrofuran; a diluent is pure water or a mixed solution of pure water and any one or two of methanol, acetonitrile, ethanol and tetrahydrofuran. The method can be applied to determination of carmustat mesylate related substances and determination of residual quantity in drugs using 4-dimethylaminopyridine as a catalystand N, N-dimethylformamide as a refining solvent in a synthesis process.

The invention provides a high-flux integrated phosphorylated proteomics detection method. A capture skeleton is formed by a probe connected to a streptavidin plate and bait protein, a tyrosine phosphorylated protein compound is enriched, captured and subjected to enzymolysis to obtain polypeptide, and the sensitivity is improved by 300 times under the condition that the use amount of the probe isreduced by 100 times. Short-gradient rapid separation is realized by utilizing high performance liquid chromatography of a self-filling chromatographic column with the inner diameter of 150 microns; efficient detection is carried out in a DIA data acquisition mode by combining a Q Extractive HF-X mass spectrometer, and separation and detection of a single sample within 35 minutes can be realized under the condition of not losing qualitative and quantitative detection performance. Identification of transient changes of phosphorylation conditions of related signal channel proteins in cells underthe action of inhibitors with different concentrations in different EGF stimulation time is realized. And a high-flux, high-sensitivity and rapid detection method is provided for enrichment and identification of the tyrosine phosphorylated protein compound.

The invention discloses a method for determining the content of total selenium in selenium-enriched proteoglycan by high performance liquid chromatography. The invention relates to a method for producing 2, 3, 5, 6-tetramethylpiperidine, 2, 3-diaminonaphthalene is used as a chelating agent; 400 microliters of acetonitrile is used as a dispersing agent; the method comprises the following steps: diluting a chelate (4, 5-benzo kohlrabi selenium brain) of selenium (IV) and 2, 3-diaminonaphthalene by acetonitrile, directly separating by using a PFP chromatographic column, eluting by using acetonitrile, measuring the fluorescence intensity by using a fluorescence detector under the conditions that the excitation wavelength is 376 nm and the emission wavelength is 520 nm, and quantifying by usingan external standard method. The method has the characteristics of stable experimental result, less sample transfer, less reagent consumption, high recovery rate and the like.

The invention provides a detonator automatic chemical dispensing system. The detonator automatic chemical dispensing system comprises a worktable, a man-machine interaction module, one or more chemical dispensing modules, a dosage control module, a stepping module and a control module, wherein the worktable is used for fixing detonator ignition parts to be subjected to chemical dispensing; the man-machine interaction module is used for setting and displaying working parameters and a working process flow of the chemical dispensing system; one or more chemical dispensing modules are used for performing chemical dispensing on the detonator ignition parts; the dosage control module is used for controlling the single chemical dispensing amount of each chemical dispensing module; the stepping module is used for controlling the relative movement of each chemical dispensing module and the worktable to enable each chemical dispensing module to dispense chemicals into the detonator ignition part; and the control module is used for controlling the working of the chemical dispensing modules, the dosage control module and the stepping module according to the working parameters and the working process flow. The chemical dispensing system realizes the accurate positioning and the precise quantification of chemical dispensing operation; automated batch chemical dispensing of the detonator ignition parts is realized, thereby being conductive to improving production efficiency; and by controlling the single chemical dispensing amount, the amount of the chemicals dispensed into the ignition parts is kept consistent, thereby being conductive to improving the consistency of the ignition parts, the product quality of the detonator ignition parts and the qualification rate of finished products.