Interleukin-6 detection kit

Abstract

The inventio discloses an interleukin-6 detection kit. The kit includes a reagent R1 and a reagent R2; the reagent R1 includes a first buffer solution, a sealing agent and inorganic salt; and the reagent R2 includes a second buffer solution, streptavidin-modified microspheres, a biotin-labeled interleukin-6 monoclonal antibody or a biotin-labeled interleukin-6 polyclonal antibody, the sealing agent and carbohydrate. Through the introduction of a streptavidin-biotin signal amplification system, improvement can be performed on traditional latex enhanced immunoturbidimetry, detection sensitivity can be greatly enhanced, and the accurate quantification on interleukin-6 can be realized.

Description

technical field [0001] This application relates to the technical field of medical detection, in particular to an interleukin-6 detection kit. Background technique [0002] Interleukin-6, referred to as interleukin 6 (IL-6), is a cytokine and belongs to a type of interleukin. Interleukin-6 can regulate the growth and differentiation of various cells, regulate immune response, acute phase response and hematopoietic function, and play an important role in the body's anti-infection immune response. Interleukin-6 has obvious changes in various diseases, and the increased level of interleukin-6 is closely related to the active stage of the disease, the development and changes of tumors, the degree of rejection and the effect of treatment. Therefore, the detection of interleukin-6 levels in patients can be Reflect changes in the patient's condition. [0003] Because the content of interleukin-6 in human blood is very low, usually in the range of 0.5pg / ml-6pg / ml for normal people,...

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Problems solved by technology

However, ELISA can only achieve qualitative or semi-quantitative determination, and the stability and accuracy of the test results are easily disturbed; the sensitivity and accuracy of chemiluminescent detection method are significantly better than ELISA, but its cost is high. And usually it can only be applied to the closed instrument system of the manufacturer, which is not conducive to the promotion of detection items; although the latex-enhanced immunoturbidimetric method is low in cost and can be widely used in open automatic biochemical analyzers, due to the sensitivity of the detection Usually not more than 10pg / ml, it is difficult to achieve the purpose of accurately measuring interleukin-6

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