Adamantine chemiluminiscence kit for detecting mechano-growth factor and E peptide thereof and manufacturing method of kit
An adamantane chemistry and growth factor technology, applied in the field of adamantane chemiluminescence kits, can solve problems such as slow speed and inability to quantify, and achieve the effects of avoiding result deviation, fast detection, and improving work efficiency
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Embodiment 1
[0024] Example 1 The preparation method of the chemiluminescent kit for detecting force growth factor and / or its E peptide
[0025] 1) Preparation of streptavidin-coupled magnetic particle working solution
[0026]The streptavidin-coupled magnetic particle suspension was magnetically separated to remove the supernatant, and the pH was 6.8, and the Tris buffer with a concentration of 0.1 mol / L was resuspended to a concentration of 0.5 mg / mL, and the buffer contained The mass percent content is 0.5% by weight of bovine serum albumin, 0.05% by weight of Triton X-100, 0.05% of proclin 300 and 0.05% of sodium azide.
[0027] 2) Preparation of biotin-labeled MGF antibody
[0028] Take 1mg MGF mouse monoclonal antibody, add appropriate amount of PBS buffer (0.05M, pH7.0-7.6) to adjust the total antibody concentration to 1mg / ml, and put it into a dialysis bag for dialysis, changing the dialysate every 3-4 hours , replace 3-4 times, transfer the antibody to a centrifuge tube or a cry...
Embodiment 2
[0045] Example 2 The preparation method of the chemiluminescence kit for detecting force growth factor and / or its E peptide
[0046] 1) Preparation of streptavidin-coupled magnetic particle working solution
[0047] The streptavidin-coupled magnetic particle suspension was magnetically separated to remove the supernatant, and the HEPES buffer with a pH of 6.8 and a concentration of 0.2 mol / L was resuspended to a concentration of 1 mg / mL, and the buffer contained mass The percentage content is 2wt% bovine serum albumin, 0.02wt% Triton X-100, 0.05% sodium azide.
[0048] 2) Preparation of biotin-labeled MGF antibody
[0049] Take 1mg of MGF goat monoclonal antibody, add appropriate amount of PBS buffer (0.05M, pH7.0-7.6) to adjust the antibody concentration to 1mg / ml, and put it into a dialysis bag for dialysis, changing the dialysate every 3-4 hours, Replace 3-4 times, and transfer the antibody to a centrifuge tube or cryopreservation tube after dialysis;
[0050] Accurately...
Embodiment 3
[0066] Example 3 The preparation method of the chemiluminescent kit for detecting force growth factor and / or its E peptide
[0067] 1) Preparation of streptavidin-coupled magnetic particle working solution
[0068] The streptavidin-coupled magnetic particle suspension was magnetically separated to remove the supernatant, and resuspended to a concentration of 0.1 mg / mL in a phosphate buffer solution with a pH of 7.6 and a concentration of 0.01 mol / L. It contains 0.05% by weight of casein protein, 1% by weight of Brij35 and 0.5% of proclin 300 by weight.
[0069] 2) Preparation of biotin-labeled MGF-Ct24E antibody
[0070] Take 1mg of MGF-Ct24E sheep monoclonal antibody, add appropriate amount of PBS buffer (0.05M, pH7.0-7.6) to adjust the antibody concentration to 1mg / ml, and put it into a dialysis bag for dialysis, and change the dialysis every 3-4 hours solution, change it 3-4 times, and transfer the antibody to a centrifuge tube or cryopreservation tube after dialysis;
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