Noninvasive biopsy virus detection method based on high throughput gene sequencing and tagged connector
A labeling and virus technology, applied in biochemical equipment and methods, microbial measurement/testing, library creation, etc., can solve the problems of incomplete reflection of the ecological composition of virus samples and unusable sequencing data, so as to reduce the cost of sequencing, High throughput and cost reduction effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0078] The material used in this example is a mixture of 8 respiratory adenovirus and syncytial virus (RSV) samples provided by Zhujiang Hospital of Southern Medical University (simulating respiratory nasopharyngeal secretions with virus infection, and the nucleic acid types are double-stranded DNA and single-stranded DNA respectively). stranded RNA). 8 mixtures were used as 8 groups of samples, each group of samples had 10 repetitions, and a total of 80 reactions were performed. In library construction, each sample was added with a DNA tag adapter. That is, add Barcode01-10 to the first group of samples; add Barcode11-20 to the second group of samples; add Barcode21-30 to the third group of samples, and so on to Barcode80. In this example, 80 DNABarcode tag adapters were combined and verified. The present invention is not limited to these 80 tag adapter sequences, and any tag adapter sequence or any combination of multiple tag adapter sequences falls within the scope of the ...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com