The invention discloses an initial culture medium special for preventing brown
stain of strawberries, which is prepared by taking an MS culture medium containing 0.2-0.5 mg / L of 6-benayl
aminopurine (BA) and 0.1-0.2 mg / L of
naphthylacetic acid (NAA) as a basic culture medium and adding
vitamin C (VC) with the concentration of 5-15 mg / L and
brassinolide (BR) with the concentration of 0.001-0.5 mg / L, wherein the brown
stain rate in the initial strawberry
tissue culture process can be greatly reduced; and the
germination rate can be increased. The invention further discloses a method for producing
tissue culture strawberry seedlings by culturing strawberry stem tip tissues through the culture medium, which comprises the steps of taking strawberry seedlings, sterilizing, inoculating, initially culturing, sub-culturing, acclimating, carrying out
virus identification, breeding in a net
yarn greenhouse and the like. According to the invention, the brown
stain phenomenon in the initial strawberry culture can be effectively prevented; the anti-oxidant condition in the initially cultured strawberry tissues is effectively improved; and the
germination rate is increased to be above 70% from 40-60%.