The invention provides a molecular marker combination related to duck egg laying traits, an obtaining method and application, and relates to the technical field of waterfowl molecular biology. According to the present invention, a No. Z sex chromosome is amplified from a female duck genome, sequencing is performed to obtain a pure molecular marker combination related to the egg laying character of the Jinding duck, the molecular combination relates to three egg laying character related gene segments, the nucleotide sequences of the three gene segments are respectively represented by SEQ ID NO.1, SEQ ID NO.2 and SEQ ID NO.3, the molecular marker is characterized in that the 81bp position of the SEQ ID NO.1 is subjected to A81G base mutation, and the 81bp position of the SEQ ID NO.2 is subjected to A81G base mutation; the base mutation of G80A occurs at the 80bp position of the SEQ ID NO.2, and the base mutation of A127G occurs at the 127bp position of the SEQ ID NO.3. The molecular marker combination can provide a rapid, simple, convenient and low-price gene analysis method for early breeding of the egg laying performance of the Jinding ducks, is beneficial to screening out Jinding duck individuals with relatively good egg laying performance, and guides early molecular marker-assisted breeding of the egg laying performance of the Jinding ducks.