37results about How to "Short sequence length" patented technology

This invention relates to the unexpected discovery that nucleotide coding sequences coding for a truncated Epstein Barr Nuclear Antigen 1 (EBNA1t) protein (lacking the Gly-Gly-Ala domain), when in cells of mammalian origin, are associated with improved growth and increased transient gene expression when compared with cells expressing a complete EBNA1 coding sequence. The expression of EBNA1t also appear to be more stable over time.

The aim of the invention is to generate a signal for measuring distance between a sender and receiver. This is achieved in that a sequence of pulses is generated with specified time intervals between individual pulses of the sequence, said time intervals being different each time.

The invention provides a β-hairpin antimicrobial peptide with cross-chain interaction between tryptophan and histidine and a preparation method thereof. Antimicrobial peptide WHFPG, the sequence of which is shown in SEQ ID No.1. The preparation method utilizes the arrangement principle of the β-hairpin structure to form two β-side chains to attract each other through the interaction of tryptophan and histidine, and uses the PG corner as the central corner unit to obtain a stable β-hairpin two Affinity antimicrobial peptide template XWYHYPGXWYHY‑NH 2 , X=R, Y=F. The application of the antibacterial peptide in the preparation of drugs for treating infectious diseases caused by Gram-positive bacteria and / or Gram-negative bacteria. The antibacterial agent can function under both neutral pH conditions and acidic pH conditions, and can more powerfully inhibit Gram-negative bacteria under acidic pH conditions, with a therapeutic index of 211.68, and has wider clinical application potential.

A method for optimizing a pilot sequence based on an observation matrix in an uplink SCMA system, the invention relates to a method for optimizing a pilot sequence based on an observation matrix. The purpose of the present invention is to solve the problems of large pilot overhead, low detection precision and complex reconstruction in the existing method of sparse channel detection. The process is as follows: 1. Establish an observation matrix model composed of pilot sequences; 2. Set the pilot set size of registered users to N, and the signal-to-noise ratio threshold to γ; if the receiving end SNR≤γ, execute 3; if the receiving end When SNR>γ, execute step 4; step 3: calculate the block sparse observation matrix formed by the pilot sequence set in the observation matrix model; step 4: calculate the block sparse observation matrix formed by the pilot sequence set in the observation matrix model. The invention is used in the field of SCMA pilot design.

Synthesis of degradable aromatic / fatty copolymer ester by low lactic acid in situ ester exchange is carried out by mixing AAC and DO with D, L-PLA in proportion of 1:1: (0-1.0), copoly-condensing without solvent or with diphenyl ether as solvent and synthesizing the final product.

The invention provides a β-hairpin antimicrobial peptide containing D-type proline and glycine corners and a preparation method thereof. The sequence of the antimicrobial peptide WKFpG of the present invention is shown in SEQ ID No.1. The present invention uses the rigid D-Pro-Gly corner as the corner unit, symmetrically places tryptophan and lysine at the non-hydrogen bond site of the β-hairpin side chain, and uses its interaction to assist the D-Pro-Gly corner to form a hairpin Structure, designed antimicrobial peptide template XWYKYZZXWYKY‑NH 2 . The application of the antibacterial peptide in the preparation of drugs for treating infectious diseases caused by Gram-positive bacteria and / or Gram-negative bacteria. The length of the amino acid sequence of this antibacterial peptide is only 12, which reveals the influence of rigid pG turn on the biological activity of β-hairpin antibacterial peptide, and has high inhibitory effect on various Gram-negative bacteria and positive bacteria, and has low hemolytic activity , the therapeutic index reaches 110.30, which is 1.5 times higher than that of the β-hairpin antimicrobial peptide with the same side chain containing NG turn, and has the potential to become a green and efficient antibiotic substitute.

The invention belongs to the field of biotechnology, and in particular relates to an antimicrobial peptide YHX‑3 and its composition and application. The amino acid sequence of the antimicrobial peptide YHX‑3 is shown in SEQ ID No.1. The antimicrobial peptide YHX-3 provided by the present invention has broad-spectrum antibacterial activity, and is effective against Gram-positive bacteria and All Gram-negative bacteria have significant antibacterial activity; and their hemolytic activity is low, the sequence length is short, the molecular weight is small, and the chemical synthesis is less difficult. It can kill pathogenic bacteria in organisms more specifically and save scale. Cost of production.

The invention provides a β-hairpin antibacterial peptide containing asparagine and glycine turn angle and a preparation method. The sequence of the antimicrobial peptide WRFNG of the present invention is shown in SEQ ID No.1. The preparation method of the present invention uses the Asn-Gly corner as the corner unit, forms the mutual attraction of two β-side chains with the interaction of Trp and Arg, and designs the antibacterial peptide template XWYRYZZXWYRY-NH according to the arrangement principle of the β-hairpin structure 2 . X=R, Y=F, ZZ=NG. The application of the antibacterial peptide in the preparation of drugs for treating infectious diseases caused by Gram-positive bacteria and / or Gram-negative bacteria. The therapeutic index of the antimicrobial peptide of the present invention reaches 138.30, which is 1.58 times higher than WRFPG with Pro-Gly corner, and 3.55 times higher than WRFpG with D-Pro-Gly corner, and has the development potential to become a green and efficient antibiotic substitute.

The invention discloses an automatic detection method and system for the similarity of audio signals, comprising: acquiring two segments of audio signals to be detected; performing frequency domain analysis on them to find out the local peak positions of all frequency domain energy; connecting frequency domain energy The local peaks are used to form the pitch track; the significant value of each audio frame is calculated according to the position of the local peak energy in the frequency domain, and the significant average value is obtained; In the part of the threshold value, the main melody sequence of the two audio signals is obtained; the similarity matrix of the two main themes is obtained; and the dynamic programming local warping algorithm is applied to perform binarization processing to obtain a binary matrix; and then the two input audio signals are judged degree of similarity. By adopting the method or system of the present invention, the detection efficiency of the similarity degree of the audio signal is improved, the plagiarism problem in the audio transmission is solved, and the copyright management problem of the digital audio is accurately and comprehensively protected.

The invention discloses a method for preparing a degradable organic-inorganic nanometer hybrid material containing POSS (Polyhedral Oligomeric Silsesquioxanes), comprising the following steps of: (1) firstly, preparing a difunctional POSS derivative (II) by carrying out the addition reaction of amino POSS (I) and 2-hydroxyethyl acrylate through Michael; and (2) secondly, forming an etherified product by carrying out the esterification reaction of aromatic dibasic acid and aliphatic dihydric alcohol and preparing the degradable organic-inorganic nanometer hybrid material containing the difunctional POSS through the melt polycondensation reaction of the difunctional POSS derivative (II) and aliphatic chydroxy oligomer with the etherified product. The preparation method has the advantages ofuniform dispersion and difficult agglomeration of POSS in the hybrid material during preparation, effective improvement on the performances of degradable copolyester composite material, simple operation and high yield.