33 results about "Epstein barr" patented technology

The present invention relates to compositions comprising analogues of purine nucleosides containing a ring-expanded (“fat”) heterocyclic ring, in place of purine, and an unmodified or modified sugar residue, pharmaceutically acceptable derivatives of such compositions, as well as methods of use thereof. In particular, these compositions may be utilized in the treatment of certain cancers, bacterial, fungal, parasitic, and viral infections, including, but not limited to, Acquired Immunodeficiency Syndrome (AIDS), hepatitis, Epstein-Barr and cytomegalovirus.

The invention provides a method for detecting pathogenic microorganisms by using a PCR (polymerase chain reaction) enzyme-linked double-cross method, belongs to the technical field of clinical medical microorganism detection, and particularly relates to a synchronous qualitative and quantitative detection method for various microorganisms. In the method, DNA (deoxyribonucleic acid) amplification, DNA hybridization and enzyme-linked immunosorbent assay reaction are used, a solid-phase surface enveloping technology is adopted, a specificity conservative segment is amplified, an amplified product is combined to a capturing probe and is adsorbed to a solid-phase surface, far-end hybridization of an enzyme-linked probe is carried out, and a quantitative color product is generated by specific antibody recognition and reaction of enzyme and a substrate. The detected pathogenic microorganisms comprise EB (Epstein Barr) viruses, giant cell and cell viruses, herpes simplex viruses 1 and herpes simplex viruses 2. Various microorganisms of a clinical sample are detected simultaneously, and four types of viruses are screened by a reaction. The method has the advantages that the time and the cost are saved, application is flexible, the repeatability, the stability, the sensitivity and the specificity are high, and the method is simple and is easy to implement. Moreover, the method is suitable for widely detecting pathogenic microorganisms and is particularly suitable for a basic medical institution.

The invention relates to primers and probes for detection of various respiratory viruses and provides a primer-probe assembly for detecting cytomegalovirus, EB (epstein-barr) virus and adenovirus. Sequences of a probe and a primer pair for detecting the cytomegalovirus, the EB virus and the adenovirus are as shown in SEQ ID NO:1-9. The invention further provides a kit for simultaneous detection of the cytomegalovirus, the EB virus and the adenovirus. By combination of multiplex PCR (polymerase chain reaction) and Taqman fluorescent probe technique, simultaneous detection of various pathogens possibly causing acute respiratory infection can be realized in one-time detection, and quickness in diagnosis and treatment guide is realized. In addition, shortening of operation time and reduction of raw material consumption are realized, and high sensitivity and specificity, effectiveness in solving of PCR pollution, high automation degree and the like are achieved.

The present invention relates to peptides immunochemically reactive with antibodies to the Epstein-Barr virus (EBV), nucleic acid sequences encoding these peptides, monoclonal antibodies against these peptides, cell lines capable of producing monoclonal antibodies and anti-idiotype antibodies. The invention also relates to recombinant vector molecules comprising a nucleic acid sequence according to the invention and host cells transformed or transfected with these vector molecules. The invention is further concerned with immunological reagents and methods for the detection of EBV or anti-EBV antibodies and a method for the amplification and detection of Epstein Barr viral nucleic acid.

The invention belongs to the field of biomedicine, in particular to a kit for detecting integrated viruses of a genome in hybrid capture. The kit comprises the following main components: (1) a solid phase medium fixed with a capture mixture of human genome DNA (Deoxyribonucleic Acid) single-chain molecules, (2) positive and negative quality control materials for identifying the hybrid capture efficiency and specificity, and (3) a primer pair and a molecular probe for carrying out real-time PCR (Polymerase Chain Reaction) identification to the captured mixture of the target DNA molecules, obtained by capture. By utilizing all or partial components in the invention, a complete integrated genome virus detection kit or an integrated genome virus detection kit with the type specificity can be assembled. The obtained virus detection kit can be used for detecting and identifying the integrated state of the genome in a certain common integrated and oncogenic viruses, such as human hepatitis B viruses, human papilloma viruses, Epstein-Barr viruses and human herpes simplex viruses, in tissue or cytology specimens.

The invention relates to a medical immunodetection method and in particular relates to a method for detecting an EB (Epstein-Barr) virus by an immunological method by using a quantum dot labelled immunochromatographic test strip. The quantum dot labelled immunochromatographic test strip is characterized in that a glass cellulose membrane A, a glass cellulose membrane B of a quantum dot labelled EB virus IgA (immunoglobulin A) monoclonal antibody, a nitrocellulose membrane and absorbent paper are stuck to a plastic board from bottom to top in sequence, wherein one end of the nitrocellulose membrane is provided with an EB virus polyclonal antibody and a rabbit anti-mouse second antibody, thereby forming a detection zone T and a quality control zone C; the quantum dot labelled EB virus IgA monoclonal antibody is arranged at the other end of the glass cellulose membrane B, corresponds to the detection zone T and the quality control zone C and is arranged at one end of a sampling point. The detection sensitivity of the method is about 1000 times higher than that of the detection method frequently used at present.

The invention provides compounds with EB (Epstein-Barr) virus and Kaposi's sarcoma-associated herpesvirus (KSHV) resisting activities and sources thereof, and a separation purification method and application of the compounds. The compounds 1-18 are separated and purified from Hypericum japonicum, wherein the compounds 1-10 are separated and purified from Hypericum japonicum collected in Dabieshan in Qichun County of Hubei Province in October, and the compounds 11-18 are separated and purified from Hypericum japonicum collected in Lushan in Lushan City of Jiangxi province. The research of the antivirus activities of the 18 compounds on the two tumorigenic herpesviruses detects that the compounds 1, 3, 4, 7 and 8 have inhibiting activities for DNA replication of EB virus; and the compounds 3, 6 and 13-17 have inhibiting activities for replication of KSHV in the pyrolysis stage.