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The invention relates to an Asia1 type foot-and-mouth disease recombinant virus without pathogenicity for a host and a preparation method and application thereof. A saving system is efficient eukaryotic plasmids which are constructed by gene engineering and can express exact foot-and-mouth disease virus genome RNA (Ribonucleic Acid), and therefore the foot-and-mouth disease recombinant virus can be constructed and prepared; vaccine strains with high titer and good antigen matching property can be prepared by using the plasmids, can be prepared into live vaccines or inactivated vaccines and can effectively stimulate bodies to produce immune response after being used for immunizing pigs and cattle, provide an immune protective effect on the pigs and the cattle and effectively protect GV and GII prevalent strains, the immune protection rate can reach 100 percent, and the median protective dose (PD50) is 6.34 to 13.59; and the recombinant virus has the advantages of high titer, high antigen matching property with the prevalent strains, wide antigen spectrum and high immune protection rate, does not have pathogenicity for pig and cattle hosts, does not form toxemia or expel toxin, and can be applied to prevention and control of Asia1 type foot-and-mouth disease viruses of China and neighboring countries.

The invention relates to a system and a method for X-ray grating contrast imaging. The system comprises an X-ray launching device, a first absorption grating, a second absorption, a detecting unit, a data processing unit and an imaging unit, wherein the X-ray launching device is used for launching X-ray beams to a detected article; the first absorption grating and the second absorption grating are positioned in the launching direction of the X-ray beams, and the X-ray refracted by the detected article forms X-ray signals with different intensity by the first absorption grating and the second absorption grating; the detecting unit is used for receiving the X-ray signals with changeable intensity and converting the X-ray signals into electric signals; and the data processing unit is used for processing and extracting the refraction angle information in the electric signals and calculating the pixel information through the refraction angle information; the imaging unit is used for constructing the image of the article. In addition, a rotating structure can also be utilized to rotate the article to realize a CT imaging mode so as to obtain refraction angles in a plurality of projection directions and the corresponding images and calculate the refraction index tomography image of the detected article by CT reconstruction algorithm. The system and the method adopt the common X-ray machine and the grating above a period of ten micron magnitude to realize the contrast imaging of a decimeter magnitude like viewing field.