50 results about "Multinucleate giant cell" patented technology

The invention discloses an AIDS biological cellular immunotherapy apparatus, and belongs to the field of medicine. The AIDS biological cellular immunotherapy apparatus is characterized in that blood and plasma separators, by which plasma, individual blood cells and multinuclear giant cells formed due to HIV parasitism are prepared; SV40LTag-pcDNA3.1 (-) and pLXSNneo-hTERT recons are constructed; combined transfection is conducted, so as to cause macrophage immortalization; the macrophage, after being subjected to amplification, is prepared in a cell cryopreservation solution until a cell concentration is 80%, and then, a purifier, which is made from a high polymer material, is poured with cells, wherein macrophage cell lines take an effect on phagocytizing HIV; the purifier and the blood and plasma separators constitute a key extracorporeal circulation device; when blood passes through the blood separator, the multinuclear giant cells which contain the HIV are filtered and removed, and subsequently, when plasma, which is separated by virtue of the plasma separator, passes through the purifier, the HIV in the plasma is phagocytized by the macrophage; and the purified plasma is mixed with the individual blood cells separated by virtue of the plasma separator, and then, the mixed plasma and individual blood cells are conveyed back, so that the purification treatment purpose of clearing the HIV inside and outside the blood cells is achieved.

The invention discloses a human cytomegalovirus immunogen fusion protein, which includes flagellin, and human cytomegalovirus envelope glycoprotein or its polypeptide fragment; the invention discloses a DNA molecule encoding the aforementioned fusion protein, and a DNA molecule comprising the DNA Molecular recombinant plasmid and recombinant expression vector; the invention discloses the preparation method and application of the fusion protein; the invention also discloses a human cytomegalovirus vaccine. The fusion protein of the invention has strong immunogenicity, can stimulate the body to produce a large amount of anti-cytomegalovirus antibodies, is used for preparing vaccines to prevent diseases caused by cytomegalovirus infection, and has good clinical application prospects.

The invention discloses a non-tumor-causing MDCK cell line for amplifying influenza virus and a screening method thereof. The cell line is MDCK‑2B2, and the preservation number is CCTCC C201323. The present invention provides a non-tumorigenic cell line MDCK-2B2 and its subclone cell line, which grow into adherent cells and / or have epithelial-like morphology and / or can support the replication of various viruses, and can amplify Higher titer virus; In addition, the present invention has found a method for screening non-tumorigenic cell lines, which can assist the judgment of tumorigenicity through the growth rate of cells and the morphological changes of cells, that is, relatively slow-growing single-pore cells, which The tumorigenicity will be significantly reduced, and the appearance of giant cells will significantly increase the tumorigenicity. When screening non-tumorigenic cell lines on a large scale, such auxiliary judgment methods can shorten time and save costs.

The invention discloses a method for increasing the number of directional differentiation of umbilical cord blood megakaryocyte progenitor cells. In this method, umbilical cord blood hematopoietic stem cells are used as sample cells, and directed differentiation is carried out in a specific medium containing resveratrol. The specific medium containing resveratrol makes the operation of directed differentiation of megakaryotic progenitor cells easier, safer and more efficient. In the process of directional differentiation and culture of umbilical cord blood hematopoietic stem cells to megakaryotic progenitor cells, resveratrol is added together with cytokines to achieve the effect of increasing the number of megakaryotic progenitor cells. The method of the present invention is easy to operate and low in cost. Cell safety is high.

An AIDS detoxification treatment instrument used in the medical field, characterized in that it prepares a blood and plasma separator capable of separating plasma, single blood cells and multinucleated giant cells formed by inhabiting HIV, and prepares HIVgp120 antibodies and HIVgp41 antibodies that can bind HIV As well as HIV gp120 and gp41 antibodies combined with goat anti-Ig, and hybridoma macrophage cell lines, as purifiers, prepared in agar gel, wrapped with polymer materials to make a purifier, and together with the separator constitute the key to the extracorporeal blood circulation device When the blood flows through the blood separator, the multinucleated giant cells containing HIV are filtered out, and then when the plasma separated by the plasma separator flows through the purifier, the HIV in it is absorbed and removed by the purifier, and the purified plasma and plasma The single blood cells separated by the separator are confluent and then reinfused into the body, so as to achieve the purpose of AIDS blood purification treatment to remove HIV inside and outside the blood cells.

The invention relates to intravenously injected cytomegalovirus human immune globulin and a preparation method thereof, and the titer of a cytomegalovirus neutralizing antibody of the human immune globulin is larger than or equal to 1:500. The preparation method comprises the steps that 1, positive plasma with the titer of the cytomegalovirus neutralizing antibody larger than or equal to 1:20 is screened out; 2, the screened-out efficient positive plasma is mixed; 3, the mixed plasma is separated through a low-temperature ethanol filter-pressing method, an immune globulin component II is separated and purified by combining an ion-exchange column chromatography method, viruses are removed through filtration, chromatography, ultrafiltration, preparation, low-pH incubation virus inactivation and nanofilm filtration, the immune globulin with the purity of 98.5%-100% is obtained through subpackaging, and the antibody titer is not lower than 1:500. The cytomegalovirus human immune globulin prepared through the preparation and production method is high in antibody titer, purity and recovery rate and capable of conducting targeted treatment on cytomegalovirus, is an effectively drug for treating recessive and dominant infection caused by the cytomegalovirus in people, is safe and reliable and has larger social benefits and economic benefits.

The invention relates to an AIDS-infected cell separator in the field of biomedicine, which is characterized in that it is made of a high-biocompatibility material that can pass through a single blood cell and chemical components but cannot pass through two or more large-volume cells that are bonded together. 150~250μm, 50~150μm, 15~40μm, 8~15μm, 5~8μm, 3~5μm, 1~2μm, correspondingly calibrated as type I~VII cell separators, simultaneously prepare HIVgp120 and CD4 antibodies, HIV infection The gp120 on the cell surface can bind other CD4+ cells by itself or under the action of gp120 antibody or CD4 antibody to form large multinucleated giant cells. Type I-II separators are used to separate super-large fungi, spirochetes, and tumor cells. HIV-infected large-volume multinucleated giant cells, multicellular aggregates, CD4+ cells susceptible to HIV infection, and AIDS-infected cells are separated and cleared in the extracorporeal circulation composed of separators and other components.

The invention discloses a method for identifying resistance of a plant to meloidogyne. The method comprises the steps of 1, grafting meloidogyne on a plant, and conducting cultivation; 2, after the step 1 finishes, taking plant root tissue, and observing the growth state of meloidogyne in the plant root tissue; 3, after the step 1 finishes, taking the plant root tissue, and calculating the number of meloidogyne in the plant root tissue; judging the resistance of the plant to meloidogyne according to the result. According to the method for identifying the resistance of the plant to meloidogyne, toluidine blue is applied to dyeing of a root-knot paraffin slice, an optimal dyeing time is found, and the growth state of the nematodes and giant cells can be clearly observed; moreover, the dyeing time is fast, and the effect is good. Aiming at the statistics on the number of the nematodes, a grinding method and a screening method are combined, an optimal grinding time is found, and convenience is brought to the statistics on the number of the nematodes. The two methods can combined into application, and the resistance of the plant to the statistics on the number of the nematodes can be expressed from two aspects of the growth state and the nematodes and the number of the nematodes.

An AIDS blood plasma purifying therapeutic instrument in the medical science field comprises a blood separator, a blood plasma separator and a blood plasma purifier; the blood plasma separator can separate blood plasma from single blood corpuscles; the blood separator can filter multinuclear giant cells formed by lodging HIV; the blood plasma purifier can prepare and amplify infinite growing hybridomas macrophage strains with phagocytosis characteristic, the hybridomas macrophage strains are mixed in a cell frozen stock solution so as to enable cell concentration to reach 80%, and the purifier is made by pouring high-molecular material; the macrophage strains can swallow HIV; the purifier, the blood separator and the blood plasma separator can form a key external cycle system; when flood flows to pass the blood separator, the multinuclear giant cells containing HIV can be filtered; when the blood plasma separated by the blood plasma separator passes the purifier, containing HIV can be removed by a cleanser; the purified blood plasma can gather with single blood cells separated by the blood plasma separator, and can be returned, thus realizing purifying treatment purpose by removing HIV inside and outside blood cells.