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Method for effectively observing tomato root knot giant cell paraffin section

A technique of paraffin sectioning and root-knot, which is applied in measuring devices, material analysis through optical means, instruments, etc., can solve the problems that can not be fully applied to tomato root-knot giant cell tissue section, tomato root-knot nematode deformation, early giant cell Missing inclusions, etc.

Inactive Publication Date: 2018-05-08
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, only Yuan Lin (2007) has published the research results of tomato root-knot giant cell paraffin section technology, but there are phenomena of tomato root-knot nematode deformation and early giant cell inclusions missing in the slice; Wang Lei (2011) only published About a tomato root knot giant cell abstract, no picture effect, no follow-up papers published
Although there have been reports on paraffin sections of other plant root-knot tissues before, different types of plant tissues have different characteristics. Although the relevant results are of reference value, they cannot be fully applied to the study of tomato root-knot giant cell tissue sections.

Method used

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  • Method for effectively observing tomato root knot giant cell paraffin section
  • Method for effectively observing tomato root knot giant cell paraffin section
  • Method for effectively observing tomato root knot giant cell paraffin section

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] A method for effectively observing tomato root-knot giant cell paraffin sections, comprising the steps of:

[0063] S1: After inoculation, wash the soil at the root of the tomato under running water for 5 minutes, collect the root knots with one side swelling and an infection angle of 40-60°, and store them in FAA fixative solution (note: collect the root knots of plants) When knotting, cut the two ends of the root knot into 0.3 cm at the upper end and 0.7 cm at the lower end according to the growth direction of the plant root tissue);

[0064] S2: Prepare a 10ml medical syringe, press the needle socket with your fingers, put the collected root knots into the syringe, pour 8ml of FAA fixative, insert the needle and the syringe with the needle pointing up, push and pull repeatedly until Observe that no air bubbles are produced in the fixative, at this time the air in the root knot has been drawn out;

[0065] S3: After vacuuming, put the root knot into a glass fixative bo...

Embodiment 2

[0085] Implementation Example 2: Obtaining the serial numbers of the best slices of tomato root knots at different days of infection.

[0086] This implementation example follows the method described in Example 1, specifically to obtain the best slice serial numbers of tomato root knots at different days of infection.

[0087] This embodiment is based on the steps of Example 1, collecting 7d, 14d, 21d, 28d and 35d tomato root knots, and making slices. 6 valid root knots were taken for each day and sectioned, and the Nikon ECLIPSE TS100 inverted microscope was used to take photos and record the whole process of giant cells and root-knot nematodes in tomato roots from production to disappearance, and giant cells appeared in each slice of the 6 valid root knots. The number of cells and the integrity of the insect body were observed and counted. The results of the implementation example show that: each root knot is cut about 220 cuts in total, of which 1 to 90 cuts in 220 cuts to...

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PUM

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Abstract

The invention discloses a method for effectively observing a tomato root knot giant cell paraffin section. The method studies three key factors affecting the effect of the tomato root knot giant cellparaffin section, and the tomato root knot giant cell paraffin section technology includes a new material-taking and embedding method and a section selection technology is established. The technologyobviously improves the working efficiency of the tomato root knot giant cell paraffin section, improves the section selection quality, and ensures the effect of the tomato root knot giant cell paraffin section. The method has the advantages of high practicality and clear technical parameters, can be used for developing a related paraffin section embedding die and a matched paraffin section specialtechnology, and can be widely promoted and applied.

Description

technical field [0001] The invention relates to the technical field of microscopic observation of plant samples, in particular to a method for paraffin sectioning plant tissues, in particular to a method for effectively observing giant cell paraffin sectioning of tomato root knots. Background technique [0002] Root-knot nematode (Meloidogyne spp.) is one of the most harmful soil-borne diseases and insect pests in the world. It can infect more than 2000 kinds of plants and bring huge losses to fruit trees, vegetables and food production all over the world. It is the most common and important crop nematode diseases (Feng Zhixin, 2001). Root-knot nematodes are endoparasitic nematodes in plant roots and live within 5-30cm of the soil surface. Generally, when the average temperature is 28°C, it takes 25 days to complete the entire life cycle of crops during the growing season, and 3-4 generations can be reproduced in one growing season. eggs (Liu Weizhi, 2000). After the seco...

Claims

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Application Information

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IPC IPC(8): G01N1/30G01N1/36G01N21/84
CPCG01N1/30G01N1/36G01N21/84G01N2021/8466
Inventor 王新荣陈芳妮连晓聪贾宁祝乐天李远纪春艳
Owner SOUTH CHINA AGRI UNIV
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