Immunity colloidal gold reagent for detecting IgG antibody of giant cells virus and its preparing method

Abstract

The reagent includes the sample pad, the binding pad, the cellulose nitrate film, the water uptake pad and the PVC back cover. The sample pad, the binding pad are adhered to the one end with PVC backcovered in sequence. The cellulose nitrate film is adhered to the middle. The water uptake pad is adhered to another end of the reagent. The binding pad is coated with the label of albumen A (or rabbit anti human IgG)-colloidal gold. The cellulose nitrate film is coated with the specificity surface film antigen of giant cells virus and chickens anti albumen A (or sheep anti rabbit IgG). The reagent features high specificity and sensitivity, simple and fast. The reagent is applicable to the detection in site within 30 minutes for the full testing procedure.

Description

technical field [0001] The invention relates to an immune colloidal gold reagent for detecting antibodies, and also relates to a preparation method of the reagent. Background technique [0002] Existing methods for detecting CMV antibody IgG mainly include enzyme-linked immunosorbent assay, latex agglutination, immunocolloidal gold diafiltration and so on. The disadvantages of the enzyme-linked immunosorbent (EIA) method are: special instruments and equipment such as microplate readers are required for use; detection operators need to undergo professional training; the operation process is relatively complicated, and the time required for detection is relatively long; the cost of detection Higher, single-person detection cannot be realized. The disadvantages of the latex agglutination method are: the operation process is relatively complicated and demanding, and one-step operation cannot be realized; the reagent needs to be stored at low temperature; ...

AI Technical Summary

Problems solved by technology

The disadvantages of the enzyme-linked immunosorbent (EIA) method are: special instruments and equipment such as microplate readers are required for use; detection operators need to undergo professional training; the operation process is relatively complicated, and the time required for detection is relatively long; the cost of detection High, unable to achieve single-person detection

The disadvantages of the latex agglutination method are: the operation process is relatively complicated and demanding, and one-step operation cannot be realized; the reagent needs to be stored at low temperature; the sensitivity is low

The disadvantages of immunocolloidal gold diafiltration are: the operation process is relatively complicated, and one-step operation cannot be realized; the reagent needs to be stored at low temperature; the cost of detection is high; the detection time is long