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A method for identifying resistance of plants to root-knot nematodes

A technology for plant roots and nematodes, which is applied in the field of identifying plant resistance to root-knot nematodes, and can solve problems such as low separation effect, high sugar consumption, and long dyeing time

Active Publication Date: 2020-05-26
BEIJING UNIV OF AGRI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, staining with safranin generally takes 8 hours, and the staining time is longer
For the counting of nematodes, Berman funnel method or sucrose centrifugation method is generally used to separate nematodes in root tissue, but the separation effect of Berman funnel method is relatively low, so it is not suitable for counting the number of nematodes
The sugar consumption ratio of the sucrose centrifugation method is relatively large, and the requirements for equipment are relatively high

Method used

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  • A method for identifying resistance of plants to root-knot nematodes
  • A method for identifying resistance of plants to root-knot nematodes

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Embodiment 1

[0048] Embodiment 1, the method for identifying plant resistance to root-knot nematode

[0049] 1. Preparation of tomato samples inoculated with root-knot nematodes

[0050] Tomatoes to be tested: tomato plants (Lycopersicon esculentum Mill cv Castlemart).

[0051] 1. Place the second instar larvae (J2) of Meloidogyne incognita in an aqueous solution to obtain a suspension (concentration: 250 larvae / mL).

[0052] 2. Take the tomato plant (5 weeks old, 4-5 true leaves) to be tested, and ensure that the soil where the tomato grows is moist, so as to facilitate the burrowing and the survival and movement of nematodes. Use a glass rod to evenly punch 4 holes 1 cm deep at a distance of 3 cm from the tomato stem, and evenly inject the suspension obtained in step 1 into the holes with an inoculation amount of 500 plants / plant, and bury the surface with a soil matrix. Seven days after inoculation, the root knots of the plants were taken for testing.

[0053] 2. Observation of paraf...

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Abstract

The invention discloses a method for identifying resistance of a plant to meloidogyne. The method comprises the steps of 1, grafting meloidogyne on a plant, and conducting cultivation; 2, after the step 1 finishes, taking plant root tissue, and observing the growth state of meloidogyne in the plant root tissue; 3, after the step 1 finishes, taking the plant root tissue, and calculating the number of meloidogyne in the plant root tissue; judging the resistance of the plant to meloidogyne according to the result. According to the method for identifying the resistance of the plant to meloidogyne, toluidine blue is applied to dyeing of a root-knot paraffin slice, an optimal dyeing time is found, and the growth state of the nematodes and giant cells can be clearly observed; moreover, the dyeing time is fast, and the effect is good. Aiming at the statistics on the number of the nematodes, a grinding method and a screening method are combined, an optimal grinding time is found, and convenience is brought to the statistics on the number of the nematodes. The two methods can combined into application, and the resistance of the plant to the statistics on the number of the nematodes can be expressed from two aspects of the growth state and the nematodes and the number of the nematodes.

Description

technical field [0001] The present invention relates to a method for identifying resistance of plants to root-knot nematodes. Background technique [0002] In recent years, root-knot nematode has become more and more serious in protected vegetable cultivation. Root-knot nematodes are obligate parasitic nematodes that complete their life cycle in plant tissues. The second instar larva (J2) enters the plant tissue from a site close to the meristematic zone and moves towards the stele of the root tip. With the development and elongation of the root tip cells, they can migrate to the mature zone of the root, find a suitable feeding site, settle down and develop into third instar larvae (J3), which stimulate the 4-5 parenchyma around the head The cells form large multinucleated giant cells with active metabolism, and gradually form vascular tissues suitable for their own living needs, so that they can absorb plant nutrients from the giant cells until they develop into adults. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
CPCA01G7/00
Inventor 王绍辉李小曼赵文超周小旋杨瑞王建立冯加平高英健赵福宽
Owner BEIJING UNIV OF AGRI
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