The invention discloses a method for observing the deposition of callose of Agrostis stolonifera blade tissues based on a paraffin slice and an aniline blue fluorescence staining technology. The method comprises the following steps: 1, preparing a medicine; 2, making the paraffin slice sequentially through the steps of material drawing, fixation, dehydration, transparentizing, paraffin impregnation, embedding, slicing, slice flatting, slice drying, staining and slice sealing, staining the obtained slice by using a fluorescence staining agent, and observing and shooting the stained slice under a fluorescence microscope. The method for observing the deposition of callose of Agrostis stolonifera blade tissues based on the paraffin slice and the aniline blue fluorescence staining technology greatly shortens the experiment time of Agrostis stolonifera paraffin slice production, overcomes the difficulty in the production of the small and fine slice of an Agrostis stolonifera blade, breaks restriction brought by the material and environment factors, rapidly and highly-efficiently completes the paraffin slice production process, and finally obtains an excellent callose fluorescence microscopic observation result.