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Separation method of aspergillus tubingensis and application of aspergillus tubingensis

A technology of Aspergillus tabin and separation method, which is applied in the directions of microorganism-based methods, separation microorganisms, chemical instruments and methods, etc., can solve the problems of limited degradation efficiency and limited types of microorganisms, and achieves efficient and simple separation methods and promotes efficient utilization. , the effect of improving efficiency

Pending Publication Date: 2022-04-01
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the types of these microorganisms are limited, and all of them need to be applied to the degradation of straw biomass by adding exogenous sources, and the degradation efficiency is severely limited.

Method used

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  • Separation method of aspergillus tubingensis and application of aspergillus tubingensis
  • Separation method of aspergillus tubingensis and application of aspergillus tubingensis
  • Separation method of aspergillus tubingensis and application of aspergillus tubingensis

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Embodiment 1

[0054] according to Figure 1-Figure 4 As shown, the present embodiment proposes that the process of isolating bacterial strains from stalks is specifically:

[0055] A method for isolating Aspergillus tubingensis, comprising the following steps:

[0056] Step S1: material preparation, clean the straw and dry it at room temperature to a water content of 6.5% to 7.3%, then cut it into straw grains with a length of 0.3 to 1.5 cm; the straw is rice straw, and the initial water content of the straw is 8.1 ~9.2%;

[0057] Step S2: Cultivate, weigh the straw grains and place them in a clean and sterilized glass petri dish, add a 1% sterilized sucrose solution, the ratio of the straw grains to the sterilized sucrose solution is 0.3g / mL, and place in the incubator Incubate in the dark at a constant temperature of 28°C for 10 days, then transfer to a Erlenmeyer flask, then place in a constant temperature incubator at 40°C, and shake at 220rpm for 1 hour to obtain a suspension;

[00...

Embodiment 2

[0069] The bacterial strain ST-3 that obtains according to the isolation method of above-mentioned Aspergillus tubingensis, reference Figure 5-Figure 6 , observe the strain morphology, hyphae and spore morphology of the strain in the aniline blue color-developing solid medium under a microscope, Bar=200 μm, and its morphological identification: cultured on the aniline blue color-developing solid medium for 5 days at 30°C, the colony diameter Reaching 68-73mm, the conidia heads are spherical or radial, and gradually spread outward from the center of the fungus, with a diameter of 155-420μm. Most of the molecular spore peduncles are born from the matrix, with smooth walls and dense apical vesicles, which are spherical or nearly spherical. The diameter is 25-62 μm, and the sporulation structure is mostly double-layered. The spores are mostly spherical or nearly spherical, and the wall is rough. From the morphological point of view, the fungus belongs to the genus Aspergillus.

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Abstract

The invention relates to a method for separating Aspergillus tubingensis and application of Aspergillus tubingensis, straws are taken as a carrier to extract microorganisms capable of degrading straws, strains can be massively propagated through culture, red brown to brown black conidia are obtained through separation, the conidia are spherical or radial, most of molecular sporophores are generated from a matrix, and most of the molecular sporophores grow from the matrix. Most spore production structures are double layers, and bacterial colonies of top sacs are densely generated; the method comprises the following steps: selecting bacterial colonies, activating the selected bacterial colonies, culturing the bacterial colonies by using an ABTS chromogenic solid culture medium and an aniline blue chromogenic solid culture medium, seeing that decolorized rings are generated after the bacterial strains are dyed, proving that the bacterial strains can secrete laccase and lignin peroxidase, amplifying ITS sequences of the screened bacterial strains, and comparing the sequences in NCBI (National Center of Biotechnology Information) to obtain similar sequence information. A phylogenetic tree is established by utilizing MEGA-X, the strain is determined to be Aspergillus tubingensis, and the Aspergillus tubingensis can be applied to lignin degradation and dye decoloration.

Description

technical field [0001] The invention belongs to the field of microbiological engineering and technology, and in particular relates to a method for isolating Aspergillus tubingensis and an application of Aspergillus tubingensis. Background technique [0002] Straw is a good biomass energy, and there are many studies on straw degradation at home and abroad, including physical methods, chemical methods and microbial methods. Lignin in straw tissue usually exists in the form of chimeras with other components, surrounding or bonding cellulose, making it difficult for water to penetrate, protecting cellulose from the attack of microorganisms or their enzymes, and becoming an effective use of cellulose and hemifibers in straw. A major obstacle to resources such as prime. [0003] At present, most straw biomass degradation methods need to remove lignin by physical or chemical methods, and then degrade cellulose and hemicellulose by adding exogenous microorganisms. This method can ...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N1/02C02F3/34C12R1/66
Inventor 李倩倩钱佳佳杨海滨
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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