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Method for staining and identifying arterial wall components

An identification method, arterial wall technology, applied in the field of staining and identification of arterial wall components

Inactive Publication Date: 2019-01-11
THE FIRST AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIV (GUANGZHOU RESPIRATORY CENT)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the existing arterial staining technology needs to be further improved

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0021] The present invention will be described in further detail below in conjunction with embodiment:

[0022] 1. Prepare the specimen:

[0023] ①Material collection: separate the arterial segment specimens to be tested;

[0024] ② Fixation: Soak the specimen in 10% formalin solution for more than 24 hours;

[0025] ③Embedding: Embed the arterial specimen in paraffin block, pay attention to the vertical embedding of the arterial segment;

[0026] ④ Slicing: cut the paraffin specimen into wax slices with a thickness of 5um;

[0027] ⑤ Patch: Paste the tissue wax slice flatly on the glass slide to make arterial slices for later use.

[0028] 2. Dye liquid preparation:

[0029] ①Ironized hematoxylin semen: weigh 0.5g of hematoxylin and 0.58g of ferric chloride, dissolve in 50ml of 95% alcohol, 0.5ml of hydrochloric acid, and 49.5ml of distilled water;

[0030] ②Aniline blue solution: weigh 2.5g of aniline blue and dissolve in 3ml of glacial acetic acid and 97ml of distilled...

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Abstract

The invention discloses a method for staining and identifying arterial wall components. The method is characterized by comprising the following steps: sequentially performing heating, dewaxing, hydrating, hematoxylin iron staining, acid aniline blue staining and acid red staining on arterial sections of paraffin, dehydrating, clarifying and sealing, and enabling different components in the arterial walls to present different colors. According to the method disclosed by the invention, the cell nucleus is stained into dark blue or black to form dots or small strips; elastic fibers are stained into black and are rope-shaped; collagen fibers are stained into blue and are rope-shaped; endothelial cell cytoplasm is stained to be rosy to form strips; and smooth muscle cell cytoplasm is stained tobe rosy to form long shuttles. According to the stained colors and morphological features, the arterial wall components are identified. Different components of the arterial walls present different colors by virtue of staining, the arterial wall components are identified by distinguishing the colors and combining morphological changes, and arterial diagnosis and treatment can be assisted.

Description

technical field [0001] The invention relates to a method, especially a method for staining and identifying arterial wall components. Background technique [0002] In the course of clinical medicine and animal research, it is often necessary to detect arteries. This involves the detection of arterial pathology, requiring the observation and identification of each component of the arterial wall. [0003] The normal arterial wall consists of three layers: (1) inner layer: close to the blood flow, also known as the intima, is the thinnest layer of the arterial wall, composed of arterial endothelial cells, which acts as a barrier and protects the artery; (2) middle layer : Located between the inner layer and the outer layer, also known as the media, is the thickest layer of the arterial wall, composed of neatly arranged layers of smooth muscle cells, elastic fibers and irregularly arranged collagen fibers, with contraction and relaxation functions; (3) Outer layer: the part in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/30G01N21/25
CPCG01N1/30G01N21/25
Inventor 李君沈粤春陆东风李新春陈爱兰冯思韵
Owner THE FIRST AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIV (GUANGZHOU RESPIRATORY CENT)
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