Sarranine and methyl violet mixed staining method for resin slices and staining solution thereof

Abstract

The invention provides a sarranine and methyl violet mixed staining method for resin slices and a staining solution thereof. The method comprises the following steps: (1) preparing a staining solution, namely preparing 0.5 mass percent of sarranine solution and 1 mass percent of methyl violet solution; and (2) respectively mixing the 0.5 mass percent of sarranine solution and the 1 mass percent of methyl violet solution according to multiple volume ratios, staining resin semithin sections, and observing the microscopic structure under a light microscope. According to the method disclosed by the invention, different histological stain requirements can be met aiming at the toluidine blue-O staining defect, and the method has the advantages of low cost and simplicity in preservation.

Description

technical field [0001] The invention belongs to the technical fields of biological science and agricultural science, and relates to a dyeing method of resin semi-thin slices and a dyeing solution thereof. Background technique [0002] Resin semi-thin sectioning is a widely used morphological research technique, and it is particularly important in related research in the fields of botany, zoology and medicine. A good dyeing method will play a finishing touch and perfectly reflect the advantages and characteristics of the resin semi-thin section technology. Toluidine blue-O is a commonly used resin semi-thin section stain, which can simultaneously visualize various structures in endosperm cells (such as cell wall, plasma membrane, nucleus, protein body and amyloid body, etc.). However, toluidine blue-O has the following multiple disadvantages: (1) the price is expensive; (2) the storage requirements are high, and the solid medicine must be kept in a closed and low temperature...

AI Technical Summary

Problems solved by technology

However, toluidine blue-O has the following multiple disadvantages: (1) the price is expensive; (2) the storage requirements are high, and the solid medicine must be kept in a closed and low temperature; (3) during the preparation process, weak base reagents such as sodium carbonate should be added as Auxiliary; (4) The solution is prone to precipitation and denaturation; (5) The dyeing time and temperature need to be controlled more precisely, otherwise it will wrinkle or split; (6) Because it is susceptible to CO in the air 2 Therefore, it is difficult to guarantee the same staining effect of slices with different development days of the same tissue, which ultimately makes the appearance of multi-image mosaic plates not good; (7) High requirements for the drying degree of resin slices , if the resin section is damp, the staining effect of this stain on the section will be greatly reduced; (8) the staining is relatively simple, and the difference in some tissue components is not obvious

The above dyeing shortcomings of toluidine blue-O are summed up by our laboratory after years of experience

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