The invention relates to a method for
electrophoresis determination of purity of recombinant human prourokinase for injection, wherein the method includes the following steps: (1) preparation of a
test sample solution: taking the recombinant human prourokinase for injection on ice, thawing, diluting with MilliQ water, adding a non reductive sample
buffer solution, placing at the temperature of 55-65 DEG C and heating for 1-5 minutes, immediately placing in an ice bath to obtain the non reductive
test sample solution; (2) preparation of a Marker solution: taking a Marker solution, thawing, placing in an 80-100 DEG C water bath for 1-5 minutes, and immediately placing in an ice bath for standby application; and (3) determination method: adopting a vertical plate
gel electrophoresis system, and detecting the
test sample solution and the Marker solution, wherein the sample loading amount of the two solutions is 10-20 [mu]L, and the instrument initial
voltage is 50-60 V; and adjusting the
voltage to 100-110 V when entering a separation gel, when a
bromophenol blue band runs to the lower edge of the gel, finishing the
electrophoresis, after
electrophoresis,
dyeing and decoloring to obtain an atlas, and calculating the purity of the test sample solution according to the atlas.