Qualitative detection method capable of distinguishing cow milk doped in human milk
A detection method, a qualitative detection technology, applied in the direction of measuring devices, preparation of test samples, material analysis through electromagnetic means, etc., can solve the problems of high price, expensive HPLC equipment, unfavorable promotion, etc., and achieve low detection cost, The effect of fast detection and low price
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[0032] (1) Preparation of reagents
[0033] 1. Sample buffer
[0034] ①1.0mol / L Tris-HCl solution (pH6.9) 80ml;
[0035] ②125ml of glycerin;
[0036] ③Bromophenol blue 5mg;
[0037] ④200ml of 10% sodium dodecyl sulfate (SDS) solution;
[0038] ⑤50ml β-mercaptoethanol;
[0039] ⑥ Make up to 1000ml with distilled water;
[0040] The pH of the final sample buffer was 6.8.
[0041] 2. Concentrated glue
[0042] ①30% acrylamide solution (containing 29.2% acrylamide and 0.8% N,N'-methylene bisacrylamide) 0.6~0.9ml;
[0043] ②1.5mol / L Tris-HCl solution (pH8.8) 0.6~0.8ml;
[0044] ③0.04~0.08ml of 10% SDS solution;
[0045] ④ 0.02~0.06ml of 10% ammonium persulfate solution;
[0046] ⑤N,N,N',N'-Tetramethylethylenediamine (TEMED) 0.003~0.007ml
[0047] ⑥ Make up to 5ml with distilled water
[0048] The final concentrated glue concentration is 3%-8%
[0049] 3. Separating glue
[0050] ①30% acrylamide solution (containing 29.2% acrylamide and 0.8% N,N'-methylenebisacrylamide) 3~5ml;
[0051] ②1.5mol / L Tris-HCl s...
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