79 results about "Dendrobine" patented technology

The invention discloses a method for one-time separation and extraction of dendrobe polysaccharide, dendrobine and erianin from dendrobium candidum. The method for extraction of dendrobe polysaccharide, dendrobine and erianin from dendrobium candidum comprises the steps of crushing of dendrobe, enzymatic hydrolysis, ultrasonic extraction, microfiltration, ultrafiltration and nano-membrane concentration, solid-liquid separation, vacuum cryogenic distillation, solvent extraction, solvent recovery, recrystallization, vacuum low-temperature drying and purification in the carbon dioxide supercritical fluid, thereby obtaining the dendrobe polysaccharide, dendrobine and erianin. The prepared product has good color, good solubility and high active ingredients.

The invention provides a process for extracting dendrobium polysaccharide and dendrobine from dendrobium officinale. The raw material used in the process comprises dendrobium officinale, cellulase (a biochemical reagent), glucose, phenol, acetone, petroleum ether and absolute ethyl alcohol. According to the process provided by the invention, the polysaccharide and dendrobine in the dendrobium officinale are extracted by using cellulase-catalyzed extraction in a combination manner; the dendrobine is obtained when the dendrobium polysaccharide is obtained; high efficiency for single extraction of the polysaccharide by an enzymic method is kept; the effects of effectively breaking cell walls are improved; dissolution of polysaccharide and the like are promoted; the process and condition are mild; the extraction rate is improved to a certain extent; and the economic benefits are relatively high.

The invention mainly relates to a method for extracting purified dendrobine from dendrobium stem. The method comprises the following steps of: firstly, extracting with acid alcohol, enriching and purifying extract by cation exchange resin, stripping with an organic solvent, and separating and preparing by HPLC (High Performance Liquid Chromatography). The stable extract with the dendrobine content not less than 90% is prepared by the method, the extract is furthered prepared by the HPLC, and the dendrobine with the purity not less than 95% is obtained. Compared with the traditional process, the invention has the advantages of high selectivity, moderate reaction condition, simple process, short production period, and high dendrobine yield and purity. By utilizing the HPLC to separate and prepare, the dendrobine loss can be reduced, the relevant matters with similar structure can be obtained, the practical value is high, and a new path by utilizing the dendrobium stem plants is provided.

The present invention relates to a method for preparing dendrobine. The method is characterized by pulverizing Herba dendrobii medicinal material, impregnating with a small amount of ammonia water, adding a high-concentration ethanol solution, ultrasonically extracting for 2-3 times, performing pressure filtration on an extractive solution with an ultrafiltration membrane, concentrating via a nanofiltration membrane, extracting a concentrated solution with acid water for 2-3 times, alkalizing an acid water layer, filtering, dissolving a precipitate in diethyl ether, mixing with a small amount of silica gel uniformly, volatizing the solvent, loading a sample, separating by partition chromatography, monitoring by thin layer chromatography, collecting an eluate, and concentrating under a reduced pressure to near dryness. The method has simple operation, low energy consumption and low investment. The product has high purity and high quality.

The invention relates to a method for seedling and planting dendrobii officmalis caulis by adopting an LED light source. LED plant growth light sources are obtained by utilizing the combination of red fluorescent powder stimulated by rare earth and a blue light chip and are applied to the growth of dendrobii officmalis caulis. The matrix of the fluorescent powder is one of aluminate matrix, vanadate matrix or nitride matrix, an emission spectrum ranges from the wavelength being 600nm to the wavelength being 660nm, an excitation spectrum ranges from the wavelength being 440nm-470nm, and the mass ratio of the fluorescent powder to organic silica gel is 1 to (4-20). The ratio of the integral area of the blue peak to the integral area of the red peak in the chip in the spectrum is 1 to (5-10). The fluorescent powder excited LED plant growth light sources can be applied to dendrobii officmalis caulis so as to promote the growth of dendrobii officmalis caulis, furthermore, the leaves are green, the newly born roots are dense, the root-stem parts are obviously sturdy, the stem height can achieve 15-28cm, the outer diameter of the stems can achieve 1-2cm, the illumination intensity can be effectively improved to conform to the sunny exposing property of dendrobii officmalis caulis, and the yield of effective ingredients, namely dendrobii officmalis caulis polysaccharide and dendrobine, can be increased, so that the method can be promoted in large scale.

The invention provides a process for extracting dendrobium polysaccharide and dendrobine from dendrobium officinale. The process comprises the following steps: (1) cleaning, drying and crushing dendrobium officinale; (2) carrying out supercritical CO2 extraction on the dendrobium nobile powder to obtain an extracting solution A; (3) extracting the extracting solution A, concentrating under reducedpressure, and drying to obtain dendrobine; (4) carrying out centrifugal separation after dendrobium residue water impregnation, and evaporating and concentrating the supernatant to obtain an extracting solution B; (5) carrying out suction filtration on the alcohol precipitation solution of the extracting solution B and then keeping precipitates; (6) dissolving the precipitates in distilled water,adding protease for enzymolysis, adding a trichloroacetic acid solution after enzymolysis, standing for precipitation, filtering and collecting filtrate; and (7) adding activated carbon into the filtrate, standing, filtering, collecting the filtrate, and carrying out freeze drying on the filtrate to obtain the dendrobium polysaccharide. According to the method, step-by-step extraction of the dendrobium polysaccharide and the dendrobine in the dendrobium officinale is achieved, the extraction rate of the dendrobium polysaccharide and the dendrobine is high, and the purity of the obtained dendrobium polysaccharide and the dendrobine is high.

The invention discloses a planting method for dendrobium officinale. The planting method includes the following steps that firstly, a seedling growing foundation is prepared; secondly, a seedling pot is prepared; thirdly, field planting is conducted; fourthly, later-period management is conducted, wherein the later-period management includes watering, fertilizing and disease and pest prevention; fifthly, harvesting is conducted. According to disease and pest prevention, an isolation ditch is formed in the periphery of a seedbed for surrounding the seedbed, the width of the isolation ditch is 30-50 cm, and the isolation ditch is filled with water or oil; carbendazim diluted 1000-fold liquid with the volume concentration of 50 percent is used for the dendrobium black spot disease happening from March to May, and spraying is conducted 2-3 times a month; carbendazim diluted 1000-fold liquid with the volume concentration of 50 percent or thiophanate methyl 1000-fold liquid with the volume concentration of 50 percent is used for the dendrobium anthracnose happening from January to May, and spraying is conducted 2-3 times a month. According to the planting method for the dendrobium officinale, the survival rate of the dendrobium officinale is increased, the content of various nutrition components, dendrobine, polysaccharide and coarse fibers of the dendrobium officinale planted through the method is high, in addition, diseases and pests in the planting process are obviously reduced, the use amount of pesticide is reduced, and the edible safety of the dendrobium officinale is improved.

The invention discloses a method for preparing dendrobine of which the content is more than 95%. The method comprises the following steps: step 1, acquiring dendrobium stems, adding an ethanol solution to the dendrobium stems for reflux extraction, adding ethanol solution again for reflux extraction, and combining extraction solutions, and concentrating the combined extraction solutions so as to obtain pasty extracts; step 2, adding an HCl solution to the pasty extracts, stirring the HCl solution and the pasty extracts, extracting dendrobine, and leaching the dendrobine so as to obtain an HCl extraction solution; step 3, repeating the step 2 until no alkaloid is detected in the HCl extraction solution; step 4, performing chloroform extraction on the HCl extraction solution for three times, alkalizing the HCl extraction solution by ammonium hydroxide until the pH value of the HCl extraction solution is 8, and performing chloroform extraction on the alkalized HCI extraction solution to obtain dendrobine; step 5, evaporating the chloroform, and retreating the step 2, step 3 and the step 4, and then evaporating the chloroform again to obtain crude dendrobine; step 6, dissolving the crude dendrobine in a small volume of ethyl ether, placing the ethyl ether with the crude dendrobine under minus 20 DEG C for crystallization, and repeating crystallization on the precipitated dendrobine crystals in the ethyl ether twice so as to obtain refined dendrobine of which the content is more than 95%. The method disclosed by the invention is simple and easy to operate, low in cost, environment-friendly, and high in purification efficiency.

The invention relates to a preparation method of a dendrobium polysaccharide beverage and a beverage adopting the same. The method comprises the unit processes: preprocessing; crushing; repeated enzymolysis leaching, colloid milling processing and filtering; batching; high-pressure homogenizing and mixing; filling and sterilization. According to the invention, by selecting a complex enzyme to carry out repeated enzymolysis and colloid milling processing on dendrobium officinale so as to break a tight structure of cell walls without influencing structures of functional ingredients of dendrobium officinale, the functional ingredients such as dendrobine, amino acid, polysaccharide and the like are easier to extract, and extraction rates of the functional ingredients of the dendrobium officinale are greatly improved, wherein the yield of the dendrobium polysaccharide can reach over 18%, and the yield of the dendrobine can reach over 0.3%. The preparation method disclosed by the invention is simple to operate and is energy-saving and environment-friendly, production cost can be greatly reduced, and the prepared dendrobium polysaccharide beverage is rich in content of the functional ingredients, is stable in product quality and can be stored for a long time without generating precipitates or the layering phenomenon.

The invention discloses a dendrobium nobile oral liquid for improving eyesight. The dendrobium nobile oral liquid is composed of the following traditional Chinese medicinal materials in percentage by weight: 40% of dendrobium nobile, 10% of semen cassiae, 10% of mulberry leaves, 10% of honeysuckle, 10% of mother chrysanthemum, 10% of barbary wolfberry fruit, and 10% of semen cuscutae. The dendrobium nobile oral liquid disclosed by the invention is an ideal daily healthcare product which takes the seven traditional Chinese medicinal materials of dendrobium nobile, semen cassiae, mulberry leaves, honeysuckle, mother chrysanthemum, barbary wolfberry fruit and semen cuscutae as raw materials, as well as is capable of reducing the lens turbidity of eyes, and positively relieving eye diseases, protecting eyes and improving eyesight. Dendrobium nobile is also known as dendrobium stem or dendrobium chrysanthum, and is a perennial herb; the stems of dendrobium nobile are caespitose, the upper parts of dendrobium nobile are slightly flat and slightly bend and ascend, and dendrobium nobile is 10-60 cm in height and 1.3 cm in thickness. Dendrobium nobile is a famous traditional Chinese medicine, contains alkaloids which mainly are dendrobine, nobilonine, 6-hydroxyl dendrobine and the like in stems, is sweet in taste and cold in property, and has the special effect of improving eyesight.

The invention discloses an extraction method of dendrobine, which is simple and convenient to operate and small in pollution. The method comprises the technical steps of: 1) crushing a dendrobium stem raw material to 20-80 meshes, infiltrating by alkaline water, placing in a CO2 supercritical extraction tank to extract, wherein the CO2 flow is 1-5ml / min / g raw material, selecting 90% acetone as an entrainer, wherein the flow is 0.1-0.5ml / min / g raw material, the extraction temperature is 30-60 DEG C, the pressure is 20-30MPa and the extraction time is 1-4 hours, dissolving the extract by chloroform, filtrating and recovering chloroform to obtain a crude extract; and 2) separating and purifying by high speed counter current chromatography by using chloroform, methanol and water as a solvent system for the crude extract, and collecting the fraction according to the chromatogram and decompressing and drying the fraction to obtain dendrobine. With the adoption of the method for extracting dendrobine, the purity of the product is high, and the method is easy to realize industrial amplification.

The invention relates to an extraction method of small molecular peptide composition of Dendrobium officinale Kimura et Migo. The extraction method comprises steps as follows: (1), pretreatment of raw materials; (2), denaturation of Dendrobium officinale Kimura et Migo protein; (3), composite enzymatic hydrolysis of the Dendrobium officinale Kimura et Migo protein; (4), filtering and impurity removing; (5), dewatering and drying. The small molecular peptide composition of Dendrobium officinale Kimura et Migo contains small molecular peptides which are obtained through hydrolysis of the Dendrobium officinale Kimura et Migo protein, the molecular weight of the small molecular peptides is mainly distributed in a range from 100 D to 700 D, and the small molecular peptide composition further contains polysaccharide, dendrobine, amino acid, stilbenoid, stilbenoid derivatives, vitamins and trace substances and can be applied to medicine raw materials, healthcare food and cosmetics. The molecular weight of a product is mainly distributed in a range from 100 D to 700 D, most of the obtained small molecular peptides are in micromolecular structures such as dipeptides, tripeptides, tetrapeptides and the like which are quite easily absorbed by a human body, and the small molecule peptides with the molecular weight distributed in 60-700 D accounts for 90% or higher of the total weight of the protein.

The invention relates to the technical fields of beautifying and skin care, and in particular relates to a formula of active ingredients of an anti-aging beauty mask; wild camellia oil, which is free from erucic acid and aflatoxin and is relatively low in cholesterol content, serves as one of raw materials in the formula, and the wild camellia oil is combined with dendrobium officinale; then in an environment with the presence of superoxide dismutase and hyaluronic acid, such active ingredients in the dendrobium officinale as dendrobine, dendrobium polysaccharide and like can act with oleic acid, linoleic acid and the like in the wild camellia oil, and a synergistic effect can be achieved in the environment with the co-presence of the superoxide dismutase and the hyaluronic acid, so that medicines can be effectively absorbed by skin directly to enhance skin immunity and improve skin activity; therefore, the formula can keep the skin soft and full for a long time, improve the water-retaining function of the skin, enhance a keratinization removing effect and delay the aging of the skin.

Disclosed is a dendrobium stem hydrating mask. The dendrobium stem hydrating mask is prepared from, 20-30 parts of dendrobium stem powder, 20-30 parts of a dendrobium stem extracting solution, 5-10 parts of amino acid, 20-30 parts of bee milk and 30-50 parts of water. The dendrobium stem powder and the dendrobium stem extracting solution are adopted; due to the fact that dendrobium stems contain abundant dendrobine, the mask has the pain-relieving and antipyretic effects, has the moisturizing effect, and can make the skin fine and smooth when used for a long time, and the skin quality is effectively improved; the amino acid can completely penetrate through the epithelial cell, and has the biological activity; the bee milk can promote and enhance the life vitality of the epidermis cell, and cell metabolism is improved; the components are safe, raw materials are natural, rapid generation of skin collagen can be effectively promoted, deep moisturizing is achieved, elasticity is enhanced, cutin is softened, and the skin is firmer.

The invention provides a method for producing dendrobine by inducing dendrobium candidum by salicylic acid. The method comprises the following steps: (1) planting the dendrobium candidum seeds on the surface of a 1 / 2MS culture medium; culturing under the condition that the temperature is 23-25 DEG C, the light intensity is 1500-1700lx and the light-dark ratio is 10h / 14h to obtain a dendrobium candidum tissue culture seedling; and (2) dissolving salicylic acid by 0.1% anhydrous ethanol solution to prepare salicylic acid mother liquor with the concentration of 5mmol / L; adding the salicylic acid mother liquor into the 1 / 2MS culture medium till the final concentration of salicylic acid is 145-155 mu mol / L; then, transferring the dendrobium candidum tissue culture seedling to the 1 / 2MS culture medium with salicylic acid and culturing for 29-31 days under the condition that the temperature is 26-28 DEG C, the relative humidity is 66-68%, the light intensity is 1800-2000lx and the light-dark ratio is 12h / 12h. According to the method provided by the invention, by applying small amount of salicylic acid in tissue culture, the accumulation efficiency of dendrobine in dendrobium candidum can be improved to a great extent, the investment is small, the effect is obvious, and the method is convenient and feasible.

The invention relates to the technical field of beverages, in particular to a dendrobium candidum beverage and a preparation method thereof. Through design on the compounding ratio of dendrobium candidum to giant salamander mucus, tea polyphenol and roxburgh rose fruits, the tea polyphenol and vitamin C generate synergistic effects each other under the effect of dendrobine, dendrobium polysaccharide, superoxide dismutase and hyaluronic acid, so that the health-care efficacies of the dendrobium candidum beverage are improved, the metabolism of tissue cells can be effectively promoted, the vitality of immune cells is improved, the immunity of human bodies is enhanced, the senescence speed is delayed, the weariness is alleviated, hepatic tissue cells are protected in an assisting manner, and the digestion is promoted.

The invention discloses a method of extracting dendrobine from dendrobium candidum. The method comprises the steps of: (1) grinding and adding the dendrobium candidum into water according to a material-liquid ratio of 1:(10-14) to form a product A, (2) regulating a pH (potential of hydrogen) of the product A to 9, (3) performing microwave extraction for thrice, 15-20min each time, on a product B,at power of 300-320W, and merging extracts to form a product C, (4) filtering the product C and performing vacuum concentration on a filtrate to form a product D, (5) adding 3-4 times of alcohol intothe product D, and putting at 1-4 DEG C for 10-12h to form a product E, and (6) filtering the product E, washing a sediment with alcohol for 3-4 times, and drying to the constant weight. When the technology is used for extracting the dendrobium candidum, an extraction rate of the dendrobine is high, and the exploitation and utilization of the dendrobine of the dendrobium candidum are promoted.

The invention discloses a cosmetic which comprises the following components in percentage by weight: 20-35% of dendrobium polysaccharides, 10-15% of glycerinum, 0-2 of ceramide, 42-65% of ultrapure water, 0.3-1% of dendrobine ethosomes, 0-2% of natural vitamin E, 0-1% of trans-resveratrol, 0-1% of vitamin C palmitate, 0-1% of alpha-arbutin and 0.1-1% of plant essential oil. The invention further provides a preparation method of the dendrobium polysaccharides and the dendrobine ethosomes in the cosmetic. The invention solves the technical problem of 'providing a cosmetic with dendrobium extracts (the dendrobium polysaccharides and the dendrobine ethosomes)', and the cosmetic disclosed by the invention has effects of ultraviolet resistance and oxidation resistance, meanwhile has effects of whitening skin and lightening stain, and moreover is purely natural and free of irritation.

The invention discloses two novel purposes of dendrobine, namely applications in preparation of drug for preventing or treating arthritis and protecting gastrointestinal mucosa from lesion.

The invention discloses a dendrobium nobile yellow rice wine and a preparation method thereof. The dendrobium nobile yellow rice wine is made of the following raw materials in percentage of weight: freeze-dried dendrobium nobile slices 5-25%, flowers of dendrobium nobile 1-10%, leaves of dendrobium nobile 1-10%, saccharomycetes 0.1-2%, outburst eurotium cristatum 0.1-1% and balance of glutinous rice. The present invention uses leaves of dendrobium nobile, flowers of dendrobium nobile and stems of dendrobium nobile as raw materials, supplemented by outburst eurotium cristatum and saccharomycetes for fermentation, so that the prepared yellow rice wine contains dendrobium polysaccharides, dendrobine, amino acids and other natural active substances as active ingredients and has the effects ofstrengthening the spleen and promoting appetite, protecting the liver and promoting bile secretion, enhancing physique and nourishing Yin fluid. In addition, the addition of the outburst eurotium cristatum enables the yellow rice wine to effectively regulate human metabolism and have high activities of reducing blood fat, reducing blood glucose and regulating saccharometabolism. The prepared yellow rice wine has aroma of dendrobium nobile, better taste, and significantly higher nutritional values and medicinal effects.

The invention provides a dendrobe processing method and a technical screening method of dendrobe. The dendrobe processing method is characterized by comprising a wine moistening step and a steaming step. The effective components extracted are high in content, the content of dendrobine can reach 0.303%, and the content of polysaccharides can reach 12.3%. The technical screening method is high in precision and accuracy, and interaction among factors can be inspected.

The invention discloses a hangover-relieving beverage produced using Camellia chrysantha and Caulis dendrobii as raw materials and a production method thereof. Active ingredients of the hangover-relieving beverage are composed of, by weight, 15-25 parts of Camellia chrysantha leaf or Camellia, 20-40 parts of Pueraria leaf or flower, 15-20 parts of Calendula officinalis, 10-20 parts of Dendrobium nano powder, 10-20 parts of Rhizoma imperatae and 15-30 parts of Flos chrysanthemi. The hangover-relieving beverage contains ingredients like Camellia saponin, Camellia flavone, Dendrobium polysaccharide, dendrobine, various amino acids, trace element selenium, plant protein and vitamins, thereby having efficacy of relieving hangover, clearing heat for detoxification, calming, clearing lung and brain and protecting liver, gallbladder and stomach. The active ingredients of the hangover-relieving beverage can be quickly decomposed in vivo to metabolize alcohol, have functions of promoting liver for detoxification and kidney for excretion, can restore gastrointestinal mucous membrane and normal flora and promote health of a digestive system and have biological functions of activating blood to remove stasis, lowering blood sugar, blood pressure and blood lipid, improving immunity and resisting lipid peroxidation, tumor and senility.

The invention provides a method for detecting the total alkaloid content of dendrobium candidum. Compared with the existing dendrobium candidum acid dye colorimetric method, the method first uses sanguinarine as a standard product to replace dendrobine so that a sample to be tested has the maximum absorption wavelength (410nm) the same to that of a complex produced by the reaction of the standardproduct and an acid dye. Preferably, bromophenol blue is used as the acid dye so that the background absorption value is reduced. The novel method has the advantages of high accuracy, good stability and easy reproduction. The method provides the accurate and effective detection method for studying the accumulation rule of total alkaloids of dendrobium candidum and the quality control of the dendrobium candidum total alkaloid preparation process.

The invention provides a method for producing dendrobine by inducing dendrobium candidum by jasmonic acid methyl ester. The method comprises the following steps: (1) sowing dendrobium candidum seeds on the surface of a 1 / 2MS culture medium and culturing under the conditions that the temperature is 23-25 DEG C, the light intensity is 1500-1700 lx and the light-dark ratio is 10h / 14h to obtain tissue culture seedlings of dendrobium candidum; and (2) dissolving jasmonic acid methyl ester in a 2% absolute ethyl alcohol solution to form a jasmonic acid methyl ester mother solution with the concentration of 10mmol / L, adding the jasmonic acid methyl ester mother solution into the 1 / 2MS culture medium till the final concentration of jasmonic acid methyl ester is 48-52 micromole / L, transplanting the tissue culture seedlings of dendrobium candidum to the 1 / 2MS culture medium with the jasmonic acid methyl ester mother solution and culturing for 29-31 days under the conditions that the temperature is 26-28 DEG C, the relative humidity is 66%-68%, the light intensity is 1800-2000 lx and the light-dark ratio is 12h / 12h. The method provided by the invention has the advantages that the efficiency of accumulation of dendrobine in dendrobium candidum can be increased by a large margin by adding a small quantity of jasmonic acid methyl ester during the tissue culture process, and the method is small in investment and convenient and easy to carry out and has remarkable effects.

The invention discloses a composition for the Alzheimer's disease, and further discloses a processing method of the composition. The processing method comprises the following steps: preparing huperzia serrata coarse powder from huperzia serrata plant; preparing dendrobium candidum coarse powder from dendrobium candidum; preparing lycium barbarum coarse powder from lycium barbarum; preparing anthocyanin coarse powder from purple sweet potato; preparing the obtained huperzia serrata coarse powder, dendrobium candidum coarse powder, lycium barbarum coarse powder and anthocyanin coarse powder into tablets or capsules according to proportion. Polysaccharide and dendrobine of dendrobium candidum, huperzia serrata of huperzia serrata, polysaccharide and isoflavone of lycium barbarum and anthocyanin are scientifically selected for the first time, so as to develop the health product for obviously preventing and treating the Alzheimer's disease. The production process is simple, the cost is low, and the product with obvious curative effects can be obtained without purification of the chemical components. The composition is obvious and stable in curative effects on the Alzheimer's disease, and the relapse is avoided within 1-2 years after drug withdrawal.

The invention relates to a method for seedling and planting dendrobii officmalis caulis by adopting an LED light source. LED plant growth light sources are obtained by utilizing the combination of red fluorescent powder stimulated by rare earth and a blue light chip and are applied to the growth of dendrobii officmalis caulis. The matrix of the fluorescent powder is one of aluminate matrix, vanadate matrix or nitride matrix, an emission spectrum ranges from the wavelength being 600nm to the wavelength being 660nm, an excitation spectrum ranges from the wavelength being 440nm-470nm, and the mass ratio of the fluorescent powder to organic silica gel is 1 to (4-20). The ratio of the integral area of the blue peak to the integral area of the red peak in the chip in the spectrum is 1 to (5-10). The fluorescent powder excited LED plant growth light sources can be applied to dendrobii officmalis caulis so as to promote the growth of dendrobii officmalis caulis, furthermore, the leaves are green, the newly born roots are dense, the root-stem parts are obviously sturdy, the stem height can achieve 15-28cm, the outer diameter of the stems can achieve 1-2cm, the illumination intensity can be effectively improved to conform to the sunny exposing property of dendrobii officmalis caulis, and the yield of effective ingredients, namely dendrobii officmalis caulis polysaccharide and dendrobine, can be increased, so that the method can be promoted in large scale.

The invention relates to the technical field of facial soap, in particular to amino acid facial soap containing Dendrobium officinale and a preparation method of the amino acid facial soap containing the Dendrobium officinale. The facial soap comprises an amino acid surfactant, plant base oil, water, triethanolamine and Dendrobium officinale extracts, the Dendrobium officinale extracts are rich in dendrobium polysaccharides, dendrobine, polyphenols, amino acids, vitamins and the like, the dendrobium polysaccharides are natural antioxidants and immunopotentiators, and have the significant effects on aspects of obviously improving the SOD level of a human body, scavenging free radicals, resisting oxidation, preventing aging and the like; the dendrobine has the effects of relieving pain, clearing heat, carrying out bacteriostasis and the like; the polyphenols, amino acids and vitamins further play an important role in promoting skin metabolism. The Dendrobium officinale extracts can improve the effects of the facial soap in filling and supplementing water, diluting fine wrinkles, deeply nourishing skin and soothing the skin in an amino acid surfactant saponification system of the invention, and the facial soap further can make the skin tender and transparent, fine and smooth and charming with high gloss after being used insistently.

The invention relates to the technical field of tea processing and especially relates to a health tea and a processing method thereof. Through a raw material ratio and an addition order in preparation processes, through combination with rolling and molding treatment processes, active ingredients of the tea can fully contact and act with dendrobine, dendrobium polysaccharides, superoxide dismutase and hyaluronic acid so that a taste and a nutrition structure of the health tea are improved. The health tea improves immunity, inhibits tyrosinase activity, improves skin water retention and decreases a skin aging speed.

The invention discloses a mycena dendrobii F48 compound microbial inoculant and application thereof. The mycena dendrobii F48 compound microbial inoculant is prepared from the following raw materials in parts by weight: 3.5 parts of mycena dendrobii F48 pure culture, 6.5 parts of trichoderma pure culture, and an adhering agent accounting for 2 percent of the total weight of the trichoderma pure culture and the mycena dendrobii F48 pure culture. The mycena dendrobii F48 compound microbial inoculant provided by the invention can be used for promoting the growth speed of a dendrobe seedling, increasing the contents of bioactivator polysaccharide and dendrobine and meeting the requirements of developing compound sustainable agriculture, ecological agriculture and green agriculture, and is suitable for large-scale production of the dendrobe as a rare medicinal plant.

The invention provides a method for extracting dendrobine from dendrobe. The method comprises the following steps: selecting reasonable macroporous resin to adsorb and an eluant to elute to obtain a relatively pure product; and finally, carrying out low-temperature crystallization by using a dilute hydrochloric acid solution to further purify the product so as to obtain high purity dendrobine, wherein the product is detected by virtue of high performance liquid chromatography, and the content of the obtained product is over 92%. The method provided by the invention is simple in process operation and the used raw materials and reagents are low in price, so that the safety is high.