50 results about "Acid fuchsin" patented technology

The invention provides a coloring agent composition for textiles and a production method of the coloring agent composition and relates to the technical field of dyeing of the textiles, in particular to the coloring agent composition for the textiles and the production method of the coloring agent composition. The coloring agent composition is prepared by combining various dyeing raw materials, an additive, a levelling agent and a penetrating agent, wherein the dyeing raw materials are formed by blending acid violet, acid leather red and an acid fuchsin according to equal amount; the levelling agent comprises the following components in percentage by weight: 10-15 percent of methylenebis naphthalenesulfonate, 15-20 percent of lignosulfonate, 2-5 percent of carboxymethyl cellulose, 2-5 percent of urea and the balance water. The coloring agent composition and the production method thereof have the advantages of favorable dying effect and firmness for dyeing of purified cotton, blend fabric and polyester-cotton fabric, simple production process, low cost and improved work efficiency.

The invention discloses a preparation method of a CDs / TiO2 composite material, a production method of a modified EVA film, and an application of the modified EVA film. The CDs / TiO2 composite nanomaterial is prepared from melon seed shells, EVA is modified, and the modified EVA film is used as a photocatalyst to realize degradation of acid fuchsin and rhodamine B. The EVA has no water-soluble properties, so the EVA is applied to degrade the acid fuchsin in organic wastewater in order to effectively reach degradation and provide convenience for curing, cycle use and recovery of the catalyst.

The invention relates to a bacterial capsule staining method, comprising the steps: 1) dropping a droplet of sterile water on a glass slide; 2) bacteria culturing for 24 to 48 hours, selecting the bacteria with a transfering loop, and slowly coating the glass slide in water moisture state spirally from the center to the outside to keep the capsule complete; 3) natural drying or blow drying with electric wind; 4) dropping 2-3 droplets of pure methanol, fixing for 1-2 minutes, and inclining to remove the methanol; 5) dropping carbolic acid fuchsin to stain for 1-3 minutes, and covering a cover glass; and 6) absorbing the liquid surrounding the cover glass with a piece of absorbent paper, inclining the slide glass to absorb partial staining liquid under the cover glass. By improvement on the slide making method, the staining method and the operation steps, the completeness of bacterial capsule is effectively kept, the growing state of all the bacterial capsule in the specimen can be clearly displayed, the operation difficulty of capsule staining is obviously reduced, and the reliability of bacterial capsule recognition is improved.

The present invention discloses a peach rootstock south root-knot nematode resisting in-vitro rapid screening method. The method comprises the following steps of: culturing seedlings; separating 2-year-old root-knot nematodes; preparing 23% Pluronic F-127; inoculating: adopting a 6-pore cell culture plate; adding 5ml of the 23% Pluronic F-127 and 500 2-year-old root-knot nematodes into each hole and uniformly mixing; putting peach seedlings into the holes when gel is about to condense; covering by the peach seedlings with a preservative film to keep moisture, and culture; dyeing: dyeing root tips with a sodium hypochlorite-acid fuchsin-glycerinum method after inoculating for 30 hours, and tabletting after dyeing; and counting and identifying: counting up the quantity of the nematodes under a microscope and defining the disease resistance of plants according to infection quantity. The peach rootstock south root-knot nematode resisting in-vitro rapid screening method has a novel design, is simple to operate and is safe and convenient; and the problems in a test that the resistance identification of the plant root nematodes is not stable, the duration is long and the counting is complicated are solved.

The invention provides nitrogen-sulfur co-doped carbon quantum dots as well as a preparation method and an application thereof.The preparation method comprises the following steps of:1) placing acid fuchsin and citric acid into a polytetrafluoroethylene hydrothermal reaction kettle liner, adding deionized water solution, and fully stirring;2) placing the liner filled with raw materials into a reaction kettle, and then placing the liner into an oven to heat to 175-185 DEG C to react for 8-12 hours to obtain orange-yellow liquid; 3) taking out the liner after the reaction kettle is naturally cooled to room temperature, pouring solution into a 500-1000Da dialysis bag to dialyze to obtain pure aqueous solution of carbon quantum dots, and freeze-drying to obtain the target carbon quantum dots.The preparation steps of the carbon quantum dots are simple, only one-step synthesis is needed, and the treatment of a surface passivator is not needed.The prepared carbon quantum dots can be used fordetecting ClO-ion, and also can be applied to cell fluorescence imaging.

The invention discloses a method for detecting form and metabolic activity of filamentous fungi. The method comprises the following steps: (1) inoculating filamentous fungi and carrying out fermenting culture; (2) taking 100-150muL of fermentation liquor when culturing to 48-72 hours, filtering by using a mesh sieve of 300-400 meshes and rinsing to remove interference of colored impurities in the fermentation liquor; (3) putting filtered hypha into a small centrifuge tube, adding normal saline to dilute into 1-2mL, and sucking up 100-150muL of solution to evenly coat on a glass slide; (4) dropwise adding 200-250muL of improved methylene blue staining fluid and staining for 10-15 minutes; (5) dropwise adding 100-150muL of improved carbolic acid fuchsin staining fluid and staining for 5-8 minutes; (6) rinsing the slide by distilled water, and observing by a cover plate. Observation of morphology is more convenient by staining means; meanwhile, the activity difference of thalli growing in different areas at one stage can be obviously distinguished, and the physiological metabolic activity is broadly understood.

The invention belongs to the technical field of biological specimen manufacturing and in particular relates to a coccid slide specimen manufacturing method which comprises the following steps: collecting live coccid, carrying out low-temperature treating, and soaking into a 5-8% NaOH solution to carry out softening treatment; flushing, dyeing with an acid fuchsin solution, soaking with 50%, 70% and 100% alcohol solutions in sequence, finally soaking in clove oil, and drying specimens. The method provided by the invention is simple, convenient and safe, and a coccid slide specimen is clear, complete, uniform in coloring and high in quality.

The invention discloses an amphoteric adsorbent prepared from grain stillage, a preparation method for the amphoteric adsorbent and an application of the amphoteric adsorbent and belongs to the fields of brewage waste resource utilization and environmental protection. The amphoteric adsorbent is prepared through taking the grain stillage as a base material, firstly, carrying out pretreatment by using alkali, carrying out a reaction by using an anion modifier, taking an inorganic salt as a converter, and carrying out a reaction by using a cation modifier. The entire process is simple in operation and low in reaction energy consumption, and the raw materials are wide in source and are economical and readily available. The amphoteric adsorbent prepared by the preparation method has good adsorption performance and has the characteristics of low cost, cyclic utilization and no environmental pollution. The amphoteric adsorbent has the adsorption removal rate of 88% to 99.99% to one of cationic dyes such as methylene blue, basic fuchsin, crystal violet, cationic red, malachite green and neutral red and any of anionic dyes such as acid black 1, acid fuchsin, methyl orange, congo red, acid red 18 and acid orange 7 in wastewater, and the adsorption capacity to each of the cationic and anionic dyes is 1mg / g to 500mg / g.

The invention discloses a capsule staining method for streptococcus pneumonia, and relates to the field of microbiological detection. The capsule staining method comprises the following steps: (1) preparing a bacterium specimen; (2) performing smearing, namely uniformly smearing a clean slide with a small amount of bacterium specimen, scraping the bacterium specimen into a thin layer, and naturally air-drying the slide; (3) performing staining: namely adding dropwise 100 to 150 microliters of carbolic acid fuchsin staining liquor onto the slide to uniformly cover the slide, and naturally air-drying the slide; (4) performing mordanting, namely washing the slide, naturally air-drying the slide, adding dropwise a mordant to uniformly cover the slide for 3 to 5min, rinsing the slide with water, and naturally air-drying the slide; (5) performing microscopic examination. The capsule staining method for streptococcus pneumonia is simple, easy, low in time consumption and high in success rate and repeatability, and has excellent application prospect in the clinical diagnosis of pneumonia, and strains of streptococcus pneumonia can be rapidly and efficiently identified.

The invention provides acid fuchsin writing liquid decolorizer, comprising the following components by weight percent: 45-54% of gypsum, 15-18% of sodium stearate, 25-30% of magnesium sulfite, 0.5-1.5% of p-hydroxyanisole, 0.5-1.5% of pH modulator and 2-5% of metal ion removing complexing agent. The decolorizer of the invention is mainly used for wiping handwriting of teaching writing liquid, has good stability and no pollution, can rapidly discolor the handwriting of acid fuchsin writing liquid, and writing can be carried out again after discoloring without spreading handwriting. When in use, the writing liquid decolorizer is placed in tap water solution for half an hour, after that, can be used by dipping with an eraser. The writing liquid decolorizer also can be used for decoloring methyl blue writing liquid.

The invention discloses a preparation method and application of a rare earth up-conversion fluorescence probe for detecting a DNA damage marker. The preparation method comprises the following specificsteps: premixing acid fuchsin and 8-OHdG dissolved in an acid buffer solution, incubating for a period of time, then adding an up-conversion nano material NaYF4: Er, Yb(NH2-UCNPs) subjected to surface amination modification, performing full mixing, and detecting under the irradiation of 980 nm excitating light to find that the fluorescence intensity difference (F-F0) of the system at 545 nm and the blank fluorescence intensity of a sample are in direct proportion to the negative logarithm of the addition concentration of 8-OHdG in order to achieve the quantitative detection of 8-OHdG. The constructed probe has strong background interference resistance and low toxicity, and can be used for detecting the fluorescence intensity of the sample under the irradiation of 980nm exciting light. Theconstructed detection method is high in sensitivity, wide in linear range, high in selectivity, stable in system, easy and convenient to operate, free of aptamer modification materials and low in cost.

The invention discloses a cationic adsorbent prepared from grain stillage, a preparation method for the cationic adsorbent and an application of the cationic adsorbent and belongs to the fields of brewage waste resource utilization and environmental protection. The cationic adsorbent is prepared through taking the grain stillage as a base material, firstly, carrying out pretreatment by using alkali, and then, carrying out a reaction by using modifiers. The entire process is simple in operation and low in reaction energy consumption; and the raw materials for the method disclosed by the invention are wide in source and are economical and readily available. The cationic adsorbent prepared from the grain stillage, prepared by the method, has good adsorption performance; and compared with unmodified grain stillage, the cationic adsorbent has obviously improved adsorption capacity and adsorption rate to a congo-red solution under equivalent conditions. The cationic adsorbent has the characteristics of low cost, cyclic utilization and no environmental pollution. The cationic adsorbent prepared by the method has the adsorption removal rate of about 88% to 99.99% to one or more of dyes such as acid black 1, acid fuchsin, methyl orange, congo red, acid red 18 and acid orange 7 in wastewater, and the adsorption capacity to one or more of the dyes is about 1mg / g to 500mg / g.

The invention relates to a collagenous fiber Masson trichrome stain kit, a preparation method thereof and a staining method. The stain kit comprises a kit body, wherein 6 dropping bottles are contained in the kit body; different reagents are respectively contained in the 6 dropping bottles; the volumes of the dropping bottles are 5ml or 10ml; the reagents in the 6 dropping bottles are hematoxylinstaining solution, hydrochloric acid differentiation solution, back-to-blue solution, ponceau acid fuchsin solution, phosphomolybdic acid solution and aniline blue solution separately. The kit performs optimization in terms of the packaging of the kit, formulas of reagents and a staining process, further a collagenous fiber Masson trichrome stain kit based on instillation is assembled, and the kitcan be directly applied to rapid staining of small-scale tissue slices, and has the characteristics of economical efficiency, practicability, special effect and speediness.

The present invention provides a whiteboard color chalk capable of fading, and a preparation method thereof, wherein the raw materials of the whiteboard color chalk comprise, by mass, 40-45% of emulsified wax, 20-25% of stearic acid, 14-18% of talc powder, 6-10% of polyethylene glycol, 1-2% of sodium polyacrylate, 2-4% of a coloring agent, 5-6% of glycerin, and 0.6-0.8% of white carbon black, wherein the coloring agent is one or a plurality of materials selected from methyl blue, acid fuchsin and acid green 3#. According to the present invention, the preparation method is simple, and the prepared color chalk has characteristics of smooth writing and clear writing on the whiteboard, fading and no trace after erasure with the erasure agent, no stimulating effect on the skin, and green environmental protection.

The invention provides a fadable coloured chalk for a white board and a preparation method thereof. The coloured chalk comprises the following raw materials in part by mass: 40-45 parts of emulsifying wax, 20-25 parts of stearic acid, 14-18 parts of talcum powder, 6-10 parts of polyethylene glycol, 1-2 parts of sodium polyacrylate, 2-4 parts of colour former, 5-6 parts of glycerol and 0.6-0.8 part of white carbon black, wherein the colour former is one or more of methyl blue, acid fuchsin and No. 3 acid green. According to the invention, the preparation method for the coloured chalk is simple, the prepared coloured chalk can write on the white board smoothly, the writing is legible and the coloured chalk can fade by erasion of an erasing agent without any trace, and the coloured chalk has no stimulation effect on skin and is environment-friendly.

The invention discloses a natural weak-acidic dye which is composed of, by weight, 14-16 parts of methylene blue, 23-26 parts of acid fuchsin, 31-33 parts of Congo red, 10-16 parts of sodium citrate,13-18 parts of ferric ion, 2-5 parts of alginic acid, 10-12 parts of bean sprout powder, 17-20 parts of fish meal, 11-18 parts of seed liquid, 21-24 parts of kudzu powder, and 9-10 parts of houttuyniacordata. The natural weak-acidic dye is natural and odor-free, can quickly coloring an object and is not liable to discolor.

The invention provides a preparation method of a palygorskite / Zn-Mg-Cr ternary metal oxide composite photocatalyst. The preparation method is as follows: dissolving ZnCl2, MgCl2, CrCl3 in water, adding palygorskite and performing uniform stirring, and adjusting the solution pH with an alkali solution to be 8-10; then transferring the mixed solution into a hydrothermal reaction kettle, and maintaining for 20-24 h at 110-130 DEG C; performing washing with water on the obtained precipitate and performing drying to obtain palygorskite / ternary metal hydroxide; and performing calcination on the palygorskite / ternary metal hydroxide for 4-6 h at 400-600 DEG C to obtain the palygorskite / Zn-Mg-Cr ternary metal oxide composite photocatalyst. The composite photocatalyst can photo-catalytically degrademethylene blue, rhodamine B, crystal violet, malachite green and other dyes, and can adsorb heavy metal ions Pb<2+>, Cu<2+>, Ni<2+> and acid fuchsin dye in water, so that the composite photocatalystcan be used for the treatment of dye wastewater and heavy metal wastewater.

The invention discloses an adsorbent, a preparation method and application thereof. The preparation method includes: mixing carboxymethyl cellulose, gelatin and a pyrrole monomer in a mass ratio of 0.2:4:0.136-1.358, then adding chromic acid for in-situ polymerization reaction, thus obtaining the adsorbent. The prepared adsorbent can be used for adsorption of acid fuchsin. The adsorbent has a unique adsorption effect on acid fuchsin, can achieve an adsorption capacity up to 90% or more, and can be recycled repeatedly with almost unchanged removal efficiency. The adsorbent has good stability; the used raw materials are all cheap and renewable biological matrix materials, the reaction conditions are mild, no additional condition is needed to promote product synthesis, the adsorbent is easy to prepare, and the production cycle is short. And the preparation raw materials are all safe and non-toxic biological matrix materials, are easily degradable, and are free of secondary pollution.

The invention discloses a natural weak-alkaline dye, which is composed of, by weight, 23-26 parts of acid fuchsin, 14-16 parts of methyl blue, 23-26 parts of fast green, 10-16 parts of sodium citrate,13-18 parts of ferric ion, 2-5 parts of alginic acid, 10-12 parts of bean sprout powder, 17-20 parts of fish meal, 11-18 parts of seed liquid, 21-24 parts of kudzu powder, and 9-10 parts of houttuynia cordata. The natural weak-alkaline dye is natural and odor-free, can quickly coloring an object and is not liable to discolor.

The invention discloses a low-alcohol dye, which is prepared from the following ingredients in parts by weight: 14-16 parts of basic fuchsin, 23-26 parts of acid fuchsin, 31-33 parts of Congo red, 10-16 parts of sodium citrate, 13-18 parts of ferric ion, 2-5 parts of alginic acid, 10-12 parts of bean sprout powder, 17-20 parts of fish meal, 11-18 parts of seed solution, 21-24 parts of arrowroot flour and 9-10 parts of herba houttuyniae. The low-alcohol dye has the advantages that the low-alcohol dye of the technical scheme is natural, free from peculiar smell, quick in coloring and unlikely to fade.

The present invention relates to the technical field of textile dyeing, particularly to a textile yarn dyeing process. The dyeing process is characterized in that: a dyeing formula is composed of acidic heliotrope 4BNS. 3BR60, FBL, acidic bright yellow G. 20, weak acid yellow 20, 3cs, sulforhodamine B, acid fuchsin 6B, acid red XB, weak acid brilliant red B, and 10: E. 3B-E; and an additive formula is composed of acetic acid, sulfuric acid, a leveling agent, and a penetrating agent. The dyeing process includes adding the additive after running for five minutes at the water temperature of 40 DEG C during the heating process; adding the dye after running for another five minutes, raising the temperature after running for 10 minutes, raising the temperature to 100 DEG C within 60 min, keeping the temperature for 45-60 minutes, and cleaning and taking out of vats after dyeing. The beneficial effects of the dyeing process are that dyeing is solid, color is bright, the yarn has the characteristic of changing color under different light sources, and the color changing is not impacted by washing.

The invention discloses a novel neutral dye, which is composed of the following components in parts by weight: 14-16 parts of methylene blue, 31-33 parts of gentian violet, and 23-26 parts of acid fuchsin. 31-33 parts of Congo red, 13-18 parts of iron ion, 2-5 parts of alginic acid, 10-12 parts of bean sprouts powder, 17-20 parts of fish meal, 11-18 parts of seed liquid, 21-24 parts of arrowroot powder and fish 9-10 parts of Xingxue. The invention has the advantages that: the novel neutral dye of the technical proposal is naturally odorless, fast in coloring and not easy to fade.

The invention discloses natural weak-acid dyestuff. The natural weak-acid dyestuff is prepared from the following components in parts by weight: 23-26 parts of acid fuchsin, 14-16 parts of sudan III,23-26 parts of sudan IV, 10-16 parts of sodium citrate, 13-18 parts of iron ion, 2-5 parts of alginic acid, 10-12 parts of bean sprout powder, 17-20 parts of fish meal, 11-18 parts of seed solution, 21-24 parts of arrowroot flour and 9-10 parts of houttuynia cordata. The natural weak-acid dyestuff disclosed by the invention has the advantages that by adoption of the technical scheme, the natural weak-acid dyestuff is natural and odorless, and is fast in coloring and difficult to fade.

The invention discloses a natural weakly-alkaline dye which consists of the following components in part by weight: 23-26 parts of acid fuchsin, 14-16 parts of basic fuchsin, 23-26 parts of crystal violet, 10-16 parts of sodium citrate, 13-18 parts of iron ions, 2-5 parts of alginic acid, 10-12 parts of bean sprout powder, 17-20 parts of fish meal, 11-18 parts of seed solution, 21-24 parts of arrowroot powder and 9-10 parts of herba houttuyniae. The natural weakly-alkaline dye disclosed by the invention has the advantages that the natural weakly-alkaline dye in the technical scheme is natural,odor-free, fast to dye and hard to fade.

The invention discloses a novel low-alcohol dye. The novel low-alcohol dye consists of the following components in parts by weight: 14-16 parts of basic fuchsin, 23-26 parts of safranin, and 23-26 parts of acid fuchsin. 31-33 parts of Congo red, 13-18 parts of iron ion, 2-5 parts of alginic acid, 10-12 parts of bean sprouts powder, 17-20 parts of fish meal, 11-18 parts of seed liquid, 21-24 parts of arrowroot powder and fish 9-10 parts of Xingxue. The invention has the advantages that: the novel low-alcohol dye of the technical proposal is naturally odorless, fast in coloring and not easy to fade.

The invention discloses a natural alkaline dye which consists of the following components in part by weight: 23-26 parts of acid fuchsin, 14-16 parts of methylene blue, 23-26 parts of methyl green, 10-16 parts of sodium citrate, 13-18 parts of iron ions, 2-5 parts of alginic acid, 10-12 parts of bean sprout powder, 17-20 parts of fish meal, 11-18 parts of seed solution, 21-24 parts of arrowroot powder and 9-10 parts of herba houttuyniae. The natural alkaline dye disclosed by the invention has the advantages that the natural alkaline dye in the technical scheme is natural, odor-free, fast to dye and hard to fade.

The invention discloses natural acid dye, which is prepared from the following ingredients in parts by weight: 14 to 16 parts of neutral red, 23 to 26 parts of acid fuchsin, 31 to 33 parts of Congo red, 10 to 16 parts of sodium citrate, 13 to 18 parts of iron ions, 2 to 5 parts of alginic acid, 10 to 12 parts of bean sprout powder, 17 to 20 parts of fish meal, 11 to 18 parts of seed liquid, 21 to24 parts of radix puerariae flour and 9 to 10 parts of herba houttuyniae. The natural acid dye has the advantages that the natural acid dye adopting the technical scheme is natural and has no foreignsmell; the coloring is fast; the color losing cannot easily occur.

The invention provides an apoptotic cell staining method. The apoptotic cell staining method includes the steps of (1), dewaxing sections to water and staining for 0.2-5 minutes by a staining solutionI, wherein the staining solution I comprises 0.1-1 g of acid fuchsin, 0.1-2 g of ponceau, 0.1-3 g of orange G and 300ml of 0.2% acetic acid; (2), washing by an acetic acid solution, treating for 3-8minutes by a phosphomolybdic acid solution, and washing by the acetic acid solution again; (3), staining for 10-30 minutes by a staining solution II and washing by the acetic acid solution again, wherein the staining solution II comprises 0.1-1 g of brilliant green and 100 ml of 0.2% acetic acid; (4), performing dehydration and transparency treatment prior to mounting. The apoptotic cell stainingmethod can identify apoptotic cells at different phases and displaying cell apoptosis statuses comprehensively, and is simple to operate, low in staining cost and suitable for popularization and application.