463 results about "Malachite green stain" patented technology

The invention belongs to the field of synthesis of nano materials, relates to the preparation of graphene/mesoporous oxide series of nano composite material, and particularly relates to a preparation method and application of graphene/cellulose/titanium dioxide composite material. The graphene/cellulose/titanium dioxide composite material is prepared by preparing graphite oxide by utilizing an improved Hummers method, carrying out ultrasound to obtain graphene oxide, reducing graphene oxide into graphene by using sodium borohydride; and finally uniformly mixing cellulose, titanium dioxide, a surface active agent (hexadecyl trimethyl ammonium bromide) and grephene. According to the invention, the synthesis method is simple, the prepared graphene/cellulose/titanium dioxide composite material is taken as an adsorbent, and a malachite green dye solution is taken as an adsorption object, the experiment shows that the composite material has an excellent adsorption removal effect. The composite material is applied to the treatment of dyes in sewage water, has the advantages of simplicity in operation, convenience, easiness in availability, high adsorption rate and the like, and has good industrially practical value.

Compounds and procedures are provided for use in the preparation of reagents that can be used in immunoassays for Malachite Green, Leucomalachite Green and Carbinol. The compounds are Malachite Green Chromatic derivatives conjugated to several positions of the Malachite Green Chromatic structure, which in turn are conjugated to antigenic polypeptides for formation of antibodies for use in immunoassays. The antibodies produced, when employed in immunoassays with specified procedures, are found to be able to detect Malachite Green Chromatic as determined by positive results with fish tissue and water sample containing Malachite Green Chromatic that has been detected by HPLC or LC-MS.

The invention discloses an enzyme immunoassay of testing the bice green residues in animal derived food, which comprises an enzyme label plate covering bice green antigen, enzyme label bice green antibody working solution, bice green standard solution, substrate solution, substrate buffer solution, reaction termination solution, concentration washing liquid and sample dilute solution. The invention further discloses a method for using the immunoassay to test bice green residues, which comprises sample pretreatment, testing via the immunoassay, processing and analyzing result. The inventive immunoassay of bice green test uses direct competition enzyme-linked immunoassay adsorption analysis technique, with high sensitivity, high stability, simplified operation, reduced reaction time, reduced error caused by complex operation, reduced cost, wide application for testing samples and high practicality.

The invention discloses a method for treating malachite green dye waste water, which comprises the following steps of: preparing a bacterial cellulose membrane; modifying the bacterial cellulose membrane; immobilizing white rot fungi by using the modified bacterial cellulose membrane; adding the obtained immobilized white rot fungi modified bacterial cellulose membrane into 50 mL of the malachite green dye waste water; dissolving 20 to 40 mg of malachite green into 1 L of white rot fungi liquid restrictive culture medium; treating the waste water on a gas bath constant-temperature shaking table at a speed of 120 rpm for 5 days at 28 DEG C, and measuring the chroma of the waste water at intervals of 24 hours; 5 days later, discarding the treated waste water, and retaining the immobilized fungi; adding 50 mL of newly-prepared dye waste water with the same concentration, treating the waste water under the same condition, and measuring the chroma of the waste water at intervals of 24 hours; and taking 5 days as a cycle, and repeating the steps for 5 times, wherein the removal rate of the chroma of the waste water is always over 86 percent. In the method, the referred bacillus xylinus and the white rot fungi are purchased from the China General Microbiological Culture Collection Center.

The invention relates to a water body, a method for rapidly detecting the residue of malachite green and colorless malachite green in an aquatic product, belonging to the technical field of residue detection in a water body and an aquatic product. The method comprises the following steps: oscillating and mixing a sample to be detected and an extractant I uniformly; adding an extractant II, oscillating and mixing and then standing still; taking and adding an supernatant to an enrichment column; observing color change of a chromogenic reagent on the lowest layer; and determining that the sample contains the malachite green and colorless malachite green if a green circular strap is formed. The extractant I is a mixed solution of acetonitrile, dichloromethane, n-hexane and paratoluenesulfonic acid, the extractant II is a mixture of lead dioxide and acid alumina, and the sample to be detected is a water body sample to be detected or an aquatic product sample to be detected. The invention has the advantages of simple operation and fewer steps, does not need expensive instruments and equipment and can embody the convenience and the flexibility to on-site detection.

The invention discloses a natural pyrite based photocatalysis method for synergic removal of heavy metal-organic pollutants from water. The catalyst pyrite involved in the photocatalysis system is crushed, ground and sieved, and then composes a pyrite photocatalysis system together with ultraviolet. The pyrite photocatalysis technology involved in the invention can be applied to synergic removal of heavy metal Cr (VI) and organic matter malachite green from water. Under ultraviolet irradiation, the removal rates of malachite green and Cr (VI) can respectively reach 97% and 52%. The pyrite photocatalyst has good repeated use performance, can maintain good reaction activity after five times of repeated use, and is fully used. The catalytic reaction operation conditions involved in the reaction are simple, and the catalyst raw materials are cheap and easily available. Based on the pollution treatment concept of using waste to treat waste, the method provided by the invention has good application prospects.

The invention relates to a phosphorus-nitrogen-boron wood flame retardant, which is characterized in that the range of each component name and weight percentage of the compositions comprises: 70 to 80 percent of 8-sodium diborate and 18 to 30 percent of one or two of inorganic phosphorus-nitrogen compound and water soluble ammonium polyphosphate, wherein the inorganic phosphorus-nitrogen compound is one or more of ammonium phosphate, diammonium phosphate and monoammonium phosphate. A germicide with the weight percentage of between 0.7 and 3 percent or/and a staining agent with the weight percentage of between 0.7 and 7 percent can be added to the phosphorus-nitrogen-boron wood flame retardant, wherein the germicide is one or more of chlorothalonil, sanmate and tebuconazol; and the staining agent is one or more of malachite green, alkali red, alkali blue, alkali yellow and alkali black. The phosphorus-nitrogen-boron wood flame retardant can be dissolved in water and the concentration of the wood flame retardant is less than or equal to 20 percent. Wood subjected to treatment through the phosphorus-nitrogen-boron wood flame retardant has the effects of good flame retardant effect, small hygroscopicity, corrosion resistance, insect prevention, mildew prevention, more color change and so on. The phosphorus-nitrogen-boron wood flame retardant has the advantages of low toxicity, no chlorine and lower cost.

The invention discloses a method for treating dye waste water by enzyme production through mixed biomass fermenting. In the method, white rot fungi which can generate a lignin degrading enzyme system are used as a fermenting enzyme-production strain; a solid and liquid culture method is adopted, a cottonseed hull and/or paddy straw are/is used as a fermentation substrate, and lignin degrading enzyme is induced to obtain an optimal lignin degrading enzyme system; and finally, crude enzyme liquid is extracted, separated and prepared and can be used for decoloring treatment of waste water of commonly-used synthetic dye, such as Congo red, phenol red, bromophenol blue, crystal violet, malachite green and the like so as to obtain the optimal decoloring effect of dye sewage. The method is simple and is easy to operate. The enzyme system induced by the method disclosed by the invention has high enzymolysis efficiency, and the optimal decoloring effect can be achieved by adopting the enzyme system to treat the dye waste water.

The invention relates to a method for cultivating and artificial breeding plateau saline-alkali water area northern pike, aiming at providing a technology suitable for cultivating and artificial breeding the northern pike in the plateau saline-alkali water area. The method adopts first filial generation fingerling bred by wild northern pike to be cultivated into parent fish. The method is characterized in that before the northern pike fingerling is put into a pond, forage fish can be cultivated by an original pond, the northern pike fingerling with the length of 5cm is put into the pond aftertwo weeks, and the polyculture proportion between the northern pike fingerling and the forage fish is 1:200-300; when the temperature of the pond reaches to 7-8 DEG C, parent fish roe of the northernpike can be selected for semidry method artificial fertilization for 5min, and talc powder is used for abhesion after fertilization; oosperm is sterilized by malachite green solution and then enters into an incubator to be incubated; by controlling the speed and the flow rate of the water flow, the dead oosperm of the northern pike can be promoted to be discharged from a drain pipe; deep well water which contains 7-9mg/L of dissolved oxygen and has the water temperature of 12DEG C can be continuously pumped into the bottom of the incubator, and running water incubation can be carried out underthe dim light; and when the fingerling flatly swim to intake the exogenous nutrition, the fish can be fed by fish bait in time.

The invention provides an aptamer electrochemical sensor for detecting malachite green (MG). A preparation method for the electrochemical sensor comprises the steps of: the prepared graphene-chitosan compound and nanogold are modified on an electrode surface, an aptamer is connected on the nanogold through chemical action, a target object is bound onto the electrode surface through the specific binding action of the aptamer and the target and then connected with an antibody of the target object, thus constructing the biosensor with a sandwich structure. When the detected solution contains the target object, a certain amount of target objects and corresponding antibodies can be fixed on the electrode surface, so that a sensing structure is formed. Horseradish peroxidase (HRP) is modified on the antibodies, and the hydrogen peroxide can be catalyzed to be decomposed, so that the change of an electrochemical signal can be generated, and the detection on the MG can be realized through the change of the electrochemical signal. The prepared aptamer electrochemical sensor is strong in selectivity, high in sensitivity, simple and fast to operate, and suitable for detection on the MG of aquatic products.

The invention belongs to the field of biotechnology, and discloses a laccase gene as well as an encoded protein and an application thereof. The open reading frame sequence of the laccase gene fmb-L103 is shown in SEQ ID NO. 1. The amino acid sequence of the protein encoded by the laccase gene fmb-L103 disclosed by the invention is shown in SEQ ID NO. 2. The laccase gene fmb-L103 disclosed by the invention is applied to decoloration for triphenlmethane dyes. The inventor clones from the selected dead bacillus vallismortis fmb-L103 with a spore laccase activity to obtain a novel prokaryotic laccase gene fmb-L103, and realizes the heterologous efficient expression of escherichia colli expression host bacteria. The recombinase fmb-L103 can be used for efficiently catalyzing the degradation of the triphenlmethane dyes of malachite green, brilliant green and aniline blue.

The invention provides a preparation method of a xanthated loess adsorbent. The method comprises the following steps of: dispersing loess into a sodium hydrate solution to form a suspension; dropwise adding carbon bisulfide into a system, and stirring for reacting to generate a xanthated loess sodium salt; adding a soluble sodium salt into the system, and stirring for reacting to generate a xanthated loess magnesium salt; and washing, drying in vacuum, and grinding into powder to obtain the xanthated loess adsorbent. As proved by a large quantity of experiments, the xanthated loess adsorbent disclosed by the invention has a looser surface structure than that of natural loess, has a large specific surface area, has very high adsorption performance on heavy metal copper ions and malachite green in waste water, has a high adsorption capability and a large adsorption volume when applied to treatment of waste water containing heavy metal copper ions and a malachite green dye, and can be used for treating waste water containing the heavy metal copper ions and the malachite green dye.

The invention provides a cyclodextrin supramolecular polymer/Fe3O4 magnetic nanoparticle complex. The complex is prepared by the following steps: firstly, crosslinking beta-cyclodextrin with pyromellitic dianhydride to synthesize a cyclodextrin supramolecular polymer (beta-CDSP); coprecipitating the beta-CDSP and FeCl3.6H2O and FeCl2.4H2O under an alkaline condition by a one-pot method, so as to obtain the complex. An X-ray diffraction (XRD) method, a transmission electron microscope (TEM) method, a Fourier transform infrared spectroscopy (FTIR) method and a thermogravimetric analysis (TGA) method are applied for characterizing to verify that the beta-CDSP is grafted to Fe3O4 magnetic nanoparticles. The complex has the advantages of low synthesis cost, environment friendliness, no toxicity, abundant hydroxide radicals, free carboxyl and better magnetic properties, and can be used as an adsorption material for adsorption cation type triphenylmethane dye pollutants; malachite green serves as a model molecule for study on adsorption properties, and results show that the complex has a good adsorption effect on the malachite green and has a potential application prospect on the aspect of the adsorption material of environmental pollutants.

The invention discloses a magnetic molecular imprinting bionic ELISA (enzyme-linked immuno sorbent assay) detecting method. The magnetic molecular imprinting bionic ELISA detecting method includes steps of preparing magnetic molecular imprinted polymer and setting up a direct-competition ELISA method. By the detecting method, uniform-particle MG-MMIPs (malachite green-magnetic molecular imprinted polymers) are prepared by an emulsion polymerization method, adsorption performance is investigated, the MG-MMIPs is used as bionic antibody, and the directly-competitive ELISA detecting method is established by means of competitive adsorption to MG antigen and enzyme labeling MG antigen. Under the optimum reaction conditions, the standard curve sensitivity is 20.14 ugL-1, and the minimum detection limit is 0.12 ugL-1 and is lower than detection limit of 2 ppb (ng/g) of a MG rapid-detection card. Meanwhile, the method has high selectivity for MG, and cross reaction rates for two structural analogues (methyl violet and brilliant green) of the MG are 7.4% and 3.9% respectively.

The invention discloses an immunochromatographic test strip for detecting MG and a preparation process thereof. The immunochromatographic test strip for detecting MG provided in the invention comprises a sample pad containing MG antibodies marked by superparamagnetic composite particles, a cellulose nitrate membrane connected with one end of the sample pad and an absorbent connected with another end of the cellulose nitrate membrane; the cellulose nitrate membrane is coated by mutually separated detection lines and quality control lines, wherein, the detection lines contain MG antigens, and the quality control lines contain antiantibodies which can specifically bind to the MG antibodies. Experiments in the invention prove that the test strip provided in the invention has the advantages of high sensitivity, strong specificity, rapidness, simpleness and capability of realizing objective detection when is used for detecting MG.

The invention discloses an Elisa agent for detecting malachite green and a method thereof. The Elisa agent comprises a malachite green antigen or antibody coated Elisa plate, an enzyme label, a malachite green specific antibody, a malachite green standard solution, a substrate developing solution, a stop solution, a concentrated cleaning solution, enzyme label diluents and a concentrated combined solution. The Elisa agent is used for the quantitative detection of the content of the malachite green in fishes, shrimps and water samples and has the advantages of high specificity and sensitivity, easy sample pretreatment, short detection time, large sample detection amount, and the like.

Modular aptameric sensors, transduce recognition events into fluorescence changes through allosteric regulation of non-covalent interactions with a fluorophore. These sensors consist of: (a) a reporting domain, which signals the binding event of an analyte through binding to a fluorophore; (b) a recognition domain, which binds the analyte; and (c) a communication module, which serves as a conduit between recognition and signaling domains. We tested recognition regions specific for ATP, FMN and theophylline in combinations with malachite green binding aptamer as a signaling domain. In each case, we obtained a functional sensor capable of responding to an increase in analyte concentration with an increase in fluorescence. Similar constructs that consist only of natural RNA could be expressed in cells and used as sensors for intracellular imaging.

The invention relates to a malachite green collaurum detection card and production and application methods thereof, belonging to the technical field of immunology. The rapid diagnosis card comprises a case and a test strip; wherein the case is opened with a sample injecting hole and an observation window, the test strip is arranged in the case, the middle part of a PVC liner plate is superposed with a nitrocellulose membrane, the two ends are respectively superposed with an absorbent pad and a sample pad, the nitrocellulose membrane is overlapped and connected with the absorbent pad and the sample pad, a membrane containing malachite green monoclonal antibody immune collaurum compound is coated on a sample region, and a detection strip containing malachite green envelop antigen and a quality control strip containing goat anti mouse IgG are arranged on the nitrocellulose membrane. The invention is a rapid, accurate and sensitive diagnosis card, requires no special apparatus, has simple operation and can meet the detection requirements of food safety, fishery and detection mechanism.

The invention discloses a preparation method of a conductivity-controllable fly ash-based geopolymer semiconductor material. The preparation method comprises the following steps: putting fly ash, carbon black, sodium silicate nonahydrate and an aqueous solution of potassium hydroxide into a stirring device and mixing; and forming with a mould and curing to obtain the conductivity-controllable fly ash-based geopolymer semiconductor material, wherein the dosage of the sodium silicate nonahydrate, potassium hydroxide, carbon black and water accounts for 15%, 7%, 0.5-4.5% and 30-40% of the fly ash mass respectively; the stable conductivity of the prepared fly ash-based geopolymer semiconductor material can be controlled in a range from 0.00025 to 0.65 (S/m) in a curing age of 28 days; and when the prepared conductivity-controllable fly ash-based geopolymer semiconductor material is used as a novel photocatalyst for degrading malachite green organic dye, a change law that the conductivity of the material is directly proportional to the degradation rate of the dye is discovered. The preparation technology is simple, the cost is low, the discharge of three wastes is avoided in the preparation process, and the conductivity-controllable fly ash-based geopolymer semiconductor material can be used as a dye degrading high-activity catalyst.