The invention discloses a novel multi-angle and multi-mode quick switching circular optical illumination
microscopic imaging system which comprises a
laser, a single mode
fiber, a collimating lens, a scanning
galvanometer, a scanning lens, a first field lens, a focus lens, a dichroic mirror, a
total internal reflection fluorescence (TIRF)
microscope objective, a sample platform, a filter, a second field lens and a
detector, wherein the single mode
fiber, the collimating lens and the scanning
galvanometer are sequentially located on an
optical axis of an outgoing beam of the
laser; the scanning lens, the first field lens, the focus lens and the dichroic mirror are sequentially located on an
optical axis of an outgoing beam of the scanning
galvanometer; the TIRF
microscope objective and the sample platform are sequentially located on an
optical axis of a reflected beam of the dichroic mirror; the filter, the second field lens and the
detector are sequentially arranged on an optical axis of a transmission beam of the dichroic mirror; the sample platform is arranged on a focus plane of the TIRF
microscope objective; a collecting hole of the
detector is formed in a focus plane of the second field lens. On the basis of TIRF, the
microscopic imaging system disclosed by the invention realizes microscopic experimental observation which is convenient and rapid, high in resolution and low in
noise.