127 results about "Laccase activity" patented technology

The invention provides hypsizigus marmoreus and a method for establishing laccase transfer system in breeding thereof, belongs to the technical field of the hypsizigus marmoreus, and solves the problems that the hypsizigus marmoreus does not have laccase activity, has slow growing speed, longer production period and weak bacterial resistance in the prior art. The Hypsizigus marmoreus new strain Jinshan No.1 has the laccase activity, and is reserved by China General Microbiological Culture Collection Center on 12 June, 2009 with the accession number of CGMCC No. 3120. Pleurotus pulmonarius withthe laccase activity and the hypsizigus marmoreus are subjected to protoplast fusion to establish the laccase transfer system in the hypsizigus marmoreus. The hypsizigus marmoreus new strain culturedby the method has excellent properties of high laccase activity, high growing speed, short production period and the like.

The invention discloses a laccase mutant and an encoding gene and an application thereof. The laccase mutant disclosed by the invention is any one of the following protein in a) to d): a) protein as shown in a sequence 1; b) protein as shown in a sequence 3; c) protein as shown in a sequence 5; d) protein with laccase activity and obtained by substituting and / or deleting and / or adding one or multiple amino acid residues in the amino acid sequences of the proteins limited by any one of a) to c). As shown in experiments, compared with wild laccase, the laccase mutant disclosed by the invention has the advantages of high activity, wide pH tolerance range and the like. The laccase mutant disclosed by the invention is expected to be applied in such fields as wastewater treatment in pulp bleaching and dyeing and printing industry, biological detection in medicine field, food industry, organic synthesis, organic pesticide degradation, etc.

The invention relates to a decomposition agent for branch composting treatment. The microbial agent is a solid biological microbial agent which is obtained by inoculating a composite bacterial liquidcontaining active ingredients of bacillus velezensis, bacillus arabinodoxae, bacillus megaterium, bacillus kaempferiae and bacillus megatherium on a solid fermentation substrate and fermenting, wherein the effective viable count of the microbial agent is not less than 1.0 * 10 < 10 > cfu / g. The cellulase activity, the laccase activity, the manganese peroxidase activity and the lignin peroxidase activity in the solid biological agent are not lower than 100.0 U / g, 1.0 U / g, 1.0 U / g and 1.5 U / g respectively, and when the solid biological agent is used for composting treatment, the degradation efficiency of lignin and cellulose can be improved, and the composting rate and quality can be improved.

The invention discloses a laccase generation cerrena and an application thereof. The laccase generation cerrena is Cerrena unicolor GSM-01, and the registration serial number of the Cerrena unicolor at the General Microbiology Center of the China Committee for Culture Collection of Microorganisms is CGMCC No.7713. As shown in experiments, the Cerrena unicolor GSM-01CGMCC No.7713 of the invention is shake cultivated in a PD culture medium for 6 days at 25 DEG C and at a rotating speed of 180rpm (the rotating radius is 20mm), the laccase activity of per ml fermentation broth is 965.42+ / -13.71U, and the laccase activity of each mg (wet weight) of mycelium is 2822.71+ / -312.33U.

The invention relates to bacillus subtilis and a culture method and application thereof. The bacillus subtilis ASD05 is preserved in the General Microbiology Center of the China Committee for CultureCollection of Microorganisms on May 15, 2019, the preservation address is No.3, No.1 Courtyard, Beichen West Road, Chaoyang District, Beijing City, and the preservation number is CGMCC No.17807. The invention further relates to the culture method and application of the bacillus subtilis ASD05. It is found for the first time that the bacillus subtilis ASD05 has the effect of adhering microorganismsrich in straw degradation effect to the surface of straw, and meanwhile has the capacity of high yield of xylanase and pectinase, cellulase activity is low, and laccase activity is avoided.

This invention discloses a method for producing laccase by fungi co-culture. The method comprises: inoculating the suspension of Trametes sp. AH28-2 into a fermentation culture medium, culturing at 22-37 deg.C for 1-4 days, adding the suspension of Trichoderma sp. ZH1, culturing for 5-10 days, and separating to obtain laccase agent with living laccase content 6,000 U / L. The culture medium is liquid culture medium, and mainly comprises certain amounts of carbon source, nitrogen source, phosphates of potassium and sodium, sulfates of copper and iron, adenine and vitamin B. The method avoids aromatic compounds and heavy metal ions to induce, thus is safe and environmentally friendly, and has high and stable laccase activity.

The invention relates to a preparation method and an application for bionic laccase based on dipeptide. The bionic laccase based on the dipeptide takes metal ions as a catalytic active center and dipeptide as an organic framework, and has laccase-like activity; and the bionic laccase based on the dipeptide is a dipeptide-crosslinking agent-metal compound. The bionic laccase prepared by using the method provided by the invention has the following advantages: the preparation process is simple, and the preparation conditions are mild; the prepared bionic laccase has the catalytic activity of simulating natural laccase; the catalytic efficiency of the bionic laccase is far higher than the catalytic efficiency of natural laccase; and the bionic laccase has high stability and good biocompatibility. The preparation method for the series of bionic laccase based on the dipeptide has potential application in development of industrial applications of wastewater treatment, textile bleaching, foodsafety and the like.

The invention discloses immobilized laccase and a preparation method thereof, and application of the immobilized laccase in antibiotic degradation. The preparation method comprises the following steps: adding laccase into a solution containing copper ions, carrying out uniform mixing, then adding a trimesic acid solution, carrying out uniform mixing, performing centrifuging to remove a supernatant, conducting cleaning with a cleaning solution, and carrying out drying in vacuum to obtain the immobilized laccase. The method is simple to operate; the laccase can be coated in a carrier; and an organic framework structure provides shell protection for the laccase, and damage to the structure of the laccase due to external factors such as friction and shearing force in the use process is avoided. According to the invention, reduction in enzyme activity avoided, and the enzyme activity is greatly improved (the enzyme activity of the immobilized laccase obtained by the immobilization method is1.5-30 times of the activity of equivalent free laccase). The immobilized laccase disclosed by the invention has efficient catalytic degradation capability on antibiotics such as tetracycline, ampicillin, tetracycline derivatives and derivatives of ampicillin, can achieve an effect close to complete degradation in an extremely short time, and is good in reusability and free of secondary pollution.

This application relates to laccase variants and uses thereof as eco-friendly biocatalysts in various industrial processes. More in particular, the disclosure relates to a polypeptide with laccase activity comprising an amino acid sequence that is more than 80% identical to the amino acid sequence according to SEQ ID NO: 1, wherein the polypeptide comprises a non-polar amino acid, preferably an amino acid residue selected from the group consisting of proline, alanine, glycine and valine at a position corresponding to amino acid 113 of SEQ ID NO: 1.