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Decomposition agent for branch composting treatment and preparation method thereof

A decomposing agent and composting technology, applied in the field of microbial treatment, can solve the problems of harsh growth conditions and limited application of fungi, and achieve the effects of accelerating decomposing efficiency, clear targeting and high safety factor.

Pending Publication Date: 2021-03-12
INST OF AGRI ENVIRONMENT & RESOURCE SHANXI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the harsh growth conditions of fungi, their industrial application is greatly limited.

Method used

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  • Decomposition agent for branch composting treatment and preparation method thereof
  • Decomposition agent for branch composting treatment and preparation method thereof
  • Decomposition agent for branch composting treatment and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Take the preserved Bacillus Velez, Bacillus Ayerbordella, Bacillus megaterium, Bacillus Heinesi, and Bacillus stratosphere, respectively, take half of the inoculation loop and insert it into 100ml liquid beef extract peptone medium, 30 Cultivate with shaking at ℃ for 6 hours, and obtain the effective number of viable bacteria not less than 1.0×10 6 cfu / ml bacterial solution.

[0056] Pipette 1ml of Bacillus Velesi, 2ml of Bacillus Ayerbordella, 2ml of Bacillus megaterium, 4ml of Bacillus Heinesi, and 2.5ml of Bacillus Stratosphere, and inoculate into 250ml of liquid beef extract In the peptone medium, shaking culture at 30°C for 12 hours, the total number of effective viable bacteria is not less than 1.0×10 10 cfu / ml compound bacteria solution.

[0057] Weigh 2.5g of glucose, 2.5g of tryptone, 0.5g of magnesium sulfate, 0.3g of manganese sulfate, and 0.1g of calcium chloride, add them into 1000ml of distilled water, mix and dissolve evenly, and obtain a nutrient solut...

Embodiment 2

[0062] Take the preserved Bacillus Velez, Bacillus Ayerbordella, Bacillus megaterium, Bacillus Heinesi, and Bacillus stratosphere, respectively, take half of the inoculation loop and insert it into 100ml liquid beef extract peptone medium, 30 Cultivate with shaking at ℃ for 6 hours, and obtain the effective number of viable bacteria not less than 1.0×10 6 cfu / ml bacterial solution.

[0063] Pipette 5ml of Bacillus Velesi, 8ml of Bacillus Ayerbordella, 8ml of Bacillus megaterium, 15ml of Bacillus Heinesi, 10ml of Bacillus Stratosphere, and add 1000ml of liquid beef extract peptone In the culture medium, shaking culture at 30°C for 12 hours, the total effective number of viable bacteria obtained is not less than 1.7×10 10 cfu / ml compound bacteria solution.

[0064] Weigh 10 g of glucose, 10 g of tryptone, 2 g of magnesium sulfate, 1.5 g of manganese sulfate, and 0.5 g of calcium chloride, add them into 5000 ml of distilled water, mix and dissolve evenly, and obtain a nutrient ...

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Abstract

The invention relates to a decomposition agent for branch composting treatment. The microbial agent is a solid biological microbial agent which is obtained by inoculating a composite bacterial liquidcontaining active ingredients of bacillus velezensis, bacillus arabinodoxae, bacillus megaterium, bacillus kaempferiae and bacillus megatherium on a solid fermentation substrate and fermenting, wherein the effective viable count of the microbial agent is not less than 1.0 * 10 < 10 > cfu / g. The cellulase activity, the laccase activity, the manganese peroxidase activity and the lignin peroxidase activity in the solid biological agent are not lower than 100.0 U / g, 1.0 U / g, 1.0 U / g and 1.5 U / g respectively, and when the solid biological agent is used for composting treatment, the degradation efficiency of lignin and cellulose can be improved, and the composting rate and quality can be improved.

Description

technical field [0001] The invention belongs to the technical field of microbial treatment of rural livestock and poultry manure, straw, fruit tree branches and other organic wastes, and relates to a decomposing agent capable of rapidly degrading the branch waste during composting treatment, and the preparation of the decomposing agent method. Background technique [0002] my country has a long history of fruit tree cultivation, with rich varieties and wide distribution. The per capita fruit tree planting area is 80 square meters, and the total output accounts for about 17% of the global total, ranking first in the world. The huge fruit tree planting area will produce a large amount of agricultural branch waste every year. [0003] With the advancement of the construction of "Park City" and "Forest City", my country's urban green area continues to increase, with the per capita park green area reaching 14.11 square meters, and the amount of garden branch waste brought about ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F17/20C12R1/07C12R1/11
CPCC12N1/20C05F17/20Y02W30/40
Inventor 闫敏李磊杨治平张强姚怡敏朱晓萍赵琪李倩何永波黄高鉴郭军玲
Owner INST OF AGRI ENVIRONMENT & RESOURCE SHANXI ACAD OF AGRI SCI
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