174 results about "Exocellulase activity" patented technology

The invention discloses a preparation method of a bioorganic fertilizer. The method is characterized by adopting a complex microorganism bacterium agent formed by bacillus subtilis, aspergillus oryzae, trichoderma reesei, phanerochaete chrysosporium and geobacillus stearothermophilus as a decomposition agent to degrade straws to prepare the bioorganic fertilizer. The preparation method has the beneficial effects that the strain composition of the complex microorganism bacterium agent is new flora composition, which takes full advantage of the physiological activities of strains to generate cellulose, hemicellulose and biological enzymes, such as ligninase, to accelerate the decomposition process of straws, thus preparing the bioorganic fertilizer; the organic straw fertilizer prepared by the preparation method is used as a base fertilizer, so that the contents of available nitrogen, rapidly available phosphorus, potassium and organic matters in soil are higher than those of available nitrogen, rapidly available phosphorus, potassium and organic matters in the soil in which straws are directly used and fertilizers are not applied; the urease activity and cellulase activity of the soil are obviously improved compared with those of the soil in which straws are directly used and fertilizers are not applied.

The invention relates to a Talaromyces transformant comprising one or more recombinant gene, capable of producing cellulase in the absence of cellulase inducer in a glucose medium, having a cellulase activity of 2 WSU / ml or more, in 16 times or more diluted supernatant or broth.

Method for extracting oils, proteins and fermentable sugars from vegetable material in an aqueous medium, includes:a) adding water to the vegetable material;b) adding an enzyme mixture containing at least one cellulase, at least one hemicellulase, and at least one pectinase, the ratio between the pectinase activity and the cellulase activity being at least 0.14, and the ratio between the pectinase activity and the hemicellulase activity being at least 7.10−3, the pectinase activity being less than 120 μmol / min / mL;c) incubating the vegetable material and the enzyme mixture with stirring to release oils, proteins and fermentable sugars in the reaction medium;d) separating the reaction medium to obtain free oil, an aqueous phase containing proteins and fermentable sugars, and a solid phase;e) optionally separating and recycling an emulsion of free oil or aqueous phase, to the medium;f) separating the proteins and fermentable sugars from the aqueous phase.