Method for preparing high-temperature-resistant alkaline xylanase
A technology of xylanase and high temperature resistance, applied in the direction of microorganism-based methods, biochemical equipment and methods, glycosylase, etc. Wide range, xylanase impurity and other problems, to achieve the effect of enhancing the body's functionality, improving anti-stress ability, and wide range of pH value
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Embodiment 1
[0051] The thermophilic bacillus 701 that produces high-temperature-resistant alkaline xylanase provided by the present invention is a strain of thermophilic bacillus HYX0021 isolated from soil samples collected from Xueli Paper Mill in Hunan Province. After repeated ultraviolet mutagenesis and nitroso Obtained by guanidine mutagenesis screening, it is characterized by high temperature resistance and strong alkali resistance.
[0052] The strain producing thermostable alkaline xylanase provided by the present invention is specifically thermophilic Bacillus 701. The strain was deposited in the China Center for Type Culture Collection (CCTCC for short, address: School of Life Sciences, Wuhan University, Luojia Mountain, Wuchang District, Wuhan City, Hubei Province, Zip Code: 430072) on October 12, 2013, with the preservation number CCTCC M2013537.
[0053] The slant medium consists of: beef extract 3-10g, sodium chloride 5-12g, peptone 10-20g, glucose 2-5g, agar 15-20g, Chinese ...
Embodiment 2
[0066] A preparation method for high temperature resistant alkaline xylanase, comprising the steps of:
[0067] (1) Activation of strains
[0068] Inoculate the slant strain of the well-preserved thermophilic Bacillus 701 on the slant medium, culture at 37°C for 24 hours to activate the strain, and activate twice in this way;
[0069] The slant medium consists of: 3g of beef extract, 5g of sodium chloride, 10g of peptone, 2g of glucose, 15g of agar, 5g of Chinese herbal medicine powder, 1000mL of distilled water, pH 5, and sterilized at 121°C for 20 minutes;
[0070] The preparation method of described Chinese herbal medicine powder is as follows:
[0071] In parts by weight, weigh 20 parts of Astragalus membranaceus, 10 parts of Codonopsis pilosula, 10 parts of Bupleurum radix, and 10 parts of Scutellaria baicalensis, respectively crush the above-mentioned Chinese herbal medicines to a particle size below 2 mm, then uniformly mix them in a container and add 3 times the weigh...
Embodiment 3
[0099] A preparation method for high temperature resistant alkaline xylanase, comprising the steps of:
[0100] (1) Activation of strains
[0101] Inoculate the slant strain of the well-preserved thermophilic Bacillus 701 on the slant medium, culture at 41°C for 30 hours to activate the strain, and activate it 3 times;
[0102] The slant medium consists of: 6g of beef extract, 8g of sodium chloride, 15g of peptone, 5g of glucose, 20g of agar, 8g of Chinese herbal medicine powder, 1000mL of distilled water, pH 8, and sterilized at 121°C for 20 minutes;
[0103] The preparation method of described Chinese herbal medicine powder is as follows:
[0104] In parts by weight, weigh 25 parts of Radix Astragali, 15 parts of Codonopsis pilosula, 12 parts of Bupleurum, and 12 parts of Scutellaria baicalensis, respectively crush the above-mentioned Chinese herbal medicines to a particle size below 2 mm, then uniformly mix in a container and add 5 times the weight of Water, keep the temp...
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