Streptomyces albidoflavus and application thereof in apple tree rot prevention and treatment
A technology for Streptomyces albiflous and apple tree rot, which is applied in the field of Streptomyces albus and its application in the prevention and control of apple tree rot, to achieve good antibacterial effect, strong weather resistance, and the effect of maintaining the vitality of the strain
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Embodiment 1
[0058] Example 1 Isolation, screening and identification of Streptomyces albidoflavus Actin-1
[0059] 1) Separation and primary screening: Accurately weigh 10g of the orchard soil sample in Yantai City, Shandong Province, add it to a sterilized triangular flask with glass beads containing 100mL of cooled sterile water, shake and mix at 200r / min for 30min, and let stand for 5min Aspirate 1mL of the supernatant, and serially dilute to obtain 10 -3 ~10 -9 Concentration, select 10 -3 、10 -4 、10 -5 、10 -6 、10 -7 Use a pipette to draw 100 μL of each gradient suspension, spread it evenly on the screening medium plate with a spreader, and use sterile water as a control, place the plate upside down in the incubator, and incubate at 30°C for 7 days. Each treatment setting was repeated horizontally for 3 groups. According to the size of the transparent circle and the characteristics of the shape, size, sporulation, surface structure, edge structure, texture, color or soluble pigme...
Embodiment 2
[0127] Embodiment 2 Streptomyces albicans Actin-1 growth temperature and acid and alkali resistance test
[0128] Preparation of spore suspension of Streptomyces microalbubus Actin-1: inoculate Streptomyces microalbusus Actin-1 on the slant of Gao's No. 1 agar medium by streaking, culture at 30°C for 7 days until spore production; add 5 mL of sterile water, and incinerate Scrape the spores into sterile water with the bacterial inoculation needle, shake and mix well, and adjust the spore concentration to 1×10 6 cells / mL to obtain a spore suspension.
[0129] 1. Growth temperature test
[0130] Prepare 2216E liquid medium, pack in 48 500mL Erlenmeyer flasks, 100mL / bottle, sterilize at 121°C for 30min, and set aside; inoculate the above spore suspension into 36 Erlenmeyer flasks according to 5‰ (V / V) , as the treatment group, 12 Erlenmeyer flasks without inoculation as the control group, according to 0 ℃, 5 ℃, 10 ℃, 15 ℃, 20 ℃, 25 ℃, 30 ℃, 35 ℃, 40 ℃, 45 ℃, 50 ℃, 55°C temperat...
Embodiment 3
[0138] Embodiment 3 Streptomyces albicans Actin-1 broad-spectrum antibacterial verification and genetic stability test:
[0139] Carry out the bacteriostasis experiment of 11 strains of pathogenic bacteria such as the pathogenic bacteria of apple tree rot disease (apple black rot skin fungus) and the pathogenic bacteria of apple spot leaf fall disease (Alternaria apple specialization type) by Streptomyces albus Actin-1, the specific operation steps are as follows:
[0140] 1) Inoculate Streptomyces albus Actin-1 on Gaoshi No. 1 agar medium plate by streaking, and cultivate it at 30° C. for 5 days; spot the pathogenic bacteria of apple tree rot disease on the PDA medium plate, and cultivate it at 28° C. for 7 days; Obtain activated Streptomyces albicans Actin-1 and activated pathogenic bacteria;
[0141] 2) Confrontation experiment 2: draw a cross on the back of the PDA medium plate with a diameter of 90 mm, take the point of the cross as the center of the plate, and inoculate ...
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