38 results about "Streptomyces albus" patented technology

The invention provides a high-inorganic nitrogen-sourced biological compound fertilizer and a preparation method thereof. The fertilizer contains the following functional bacteria: bacillus megaterium, bacillus subtilis, saccharomyces cerevisiae, lactobacillus plantarum, Mucor abundans and streptomyces albus. The content of inorganic nitrogen of the compound fertilizer can be as high as 35 percent, while the number of live bacteria can still be up to 2*10 cfu / kg. Experiments show that the field fertilizer efficiency of the compound fertilizer is significantly improved.

The invention discloses a preparation method of a seedling culture medium of watermelon seedlings. The preparation method comprises the following steps: (1) uniformly mixing straws, rice husks, sawdust, biogas residues, mushroom residues and wine residues to obtain a fermentation medium; (2) adding a fermentation bacterial agent into the fermentation medium, and laminating, composting and fermenting to obtain a composted fermentation product, wherein the fermentation bacterial agent is mixed bacteria of saccharomycetes, actinomycetes, lactobacillus and bacillus subtilis; the saccharomycetes is pichia pastoris, the actinomycetes is streptomyces albus, the lactobacillus is lactobacillus acidophilus and the bacillus subtilis is bacillus vallismortis; (3) mixing the composted fermentation product with pearlite to obtain the seedling culture medium of the watermelon seedlings. The preparation method of the seedling culture medium of the watermelon seedlings has the advantages that the preparation method is simple, the cost is low, the cultured watermelon seedlings are stronger, the quality of the watermelon seedlings is good, the total dry weight of the watermelon seedlings is remarkably improved, the root density is remarkably improved and the like.

The present invention relates to product(s) containing living microorganism(s) suitable for soil treatment, microorganisms multiplying under different climatic and natural circumstances, as well as procedures for the production of the products, and procedures for the treatment of the soil and plants with the products. More particularly, the invention relates to a procedure for preparing the products from any of the microorganisms specified below, or from the mixture thereof. Furthermore, the invention relates to a procedure for the creation of the cultures of the microorganisms to be used. The subject invention also pertains to the microorganisms themselves. More particularly, the invention relates to a procedure for the treatment of the soil and the plants with a product containing at least one of the microorganisms selected from Azospirillum brasilense ssp. SW51 (NCAIM / P / B 001293), Azotobacter vinelandii ssp. M657 (NCAIM / P / B 001292), Pseudomonas fluorescens var. SW11 (NCAIM / P / B 001296), Bacillus polymyxa var. SW17 (NCAIM / P / B 001295), Bacillus megaterium var. M326 (NCAIM / P / B 001291), Micrococcus roseus ssp. A21 (NCAIM / P / B 001294), Bradyrhizobium japonicum var. PH25 (NCAIM / P / B 001302), and Streptomyces albus var. 0003 LP (NCAIM / P / B 001301), and furthermore the products multiplying and existing in the environment of the plant in question, containing the listed microorganisms and their production.

The invention discloses streptomyces albidoflavus and application thereof in production of epsilon polylysine, and belongs to the field of microbial fermentation engineering. The Streptomyces albus GS114 provided by the invention can grow on an agar culture medium containing more than 10 [mu] g / ml of one or more of streptomycin, gentamycin and rifamycin, shows very strong antibiotic resistance, can normally grow in a liquid culture medium with a pH value of 2.5-3.0 and can synthesize a large amount of epsilon PL, and after fermentation for 200 h, the Streptomyces albus GS114 can be used as anantibiotic. The concentration of epsilon polylysine in the fermentation liquor is 65.7 g / L, and the yield of epsilon polylysine reaches 80.0 g / L or above after fermentation is conducted for 240 h. Thestrain also has good passage stability, and is an epsilon PL industrial production strain with great potential.

The invention provides a high-inorganic nitrogen-sourced biological compound fertilizer and a preparation method thereof. The fertilizer contains the following functional bacteria: bacillus megaterium, bacillus subtilis, saccharomyces cerevisiae, lactobacillus plantarum, Mucor abundans and streptomyces albus. The content of inorganic nitrogen of the compound fertilizer can be as high as 35 percent, while the number of live bacteria can still be up to 2*10<10> cfu / kg. Experiments show that the field fertilizer efficiency of the compound fertilizer is significantly improved.

A method for improving the fermentation level of salinomycin is to reduce its response to salinomycin biosynthesis by inactivating the type I PKS biosynthesis gene cluster located at SLNWT0868‑SLNWT0920 (1,173,724bp‑1,276,916bp) in Streptomyces albicans DSMZ41398. Body competition, to achieve the increase of salinomycin production. By adopting the method of the invention, the final fermentation yield of salinomycin can be increased by more than 8 times, and the laboratory shake flask level can reach 6.3g / L.

The invention relates to a streptomyces albicans X-18 and a method for producing ε-polylysine by using the bacteria, belonging to the technical field of bioengineering. The Streptomyces albicans X‑18 is isolated and screened from soil, and has been preserved with the preservation number CGMCC No.17536. Utilize described Streptomyces albicans X-18 to ferment and produce ε-polylysine, and by improving the fermentation medium and fermentation conditions, in combination with the method of intermediate feeding and adding inducers, the fermentation of ε-polylysine The output can reach 3.5‑4.2g / L. The invention mainly uses strains with independent intellectual property rights, and uses cheap cornstarch as the main component of the fermentation medium, thereby reducing the production cost and increasing the fermentation output.

The invention relates to a mutagenized strain of streptomyces albus TUST2, and a method for using the same to produce epsilon-polylysine and a salt of the epsilon-epsilon. The strain is a high-yield mutagenized strain of epsilon-polylysine selected and bred by means of ultraviolet mutagenesis, ultraviolet-chemical mutagenesis, N ion injection mutagenesis, etc from a strain of streptomyces albus TUST2 which is capable of producing epsilon-polylysine and is screened from the earth in Hainan province of China; the mutagenized strain is resistant to 10mg / ml or above S-(2 Aminoethyl )-L-Cysteine and can generate 10 to 30 g / L acid under optimal conditions; zymotic fluid is removed of the streptomyces albus by configuration and is filtered by diatomaceous earth to obtain clear filtrate and to weak acid type ion exchange resin to obtain coarse epsilon-polylysine products which is subjected to ultrafiltration and purification to get epsilon-polylysine and the salt of the epsilon-epsilon. The distribution of molecular weight of epsilon-polylysine is between 4000 and 6500Da.

The invention discloses a method for efficiently synthesizing mequinomycin A in streptomyces albus. The invention is based on the method of introducing site-directed mutant A penicillin binding protein gene into the heterologous expression host Streptomyces.albus of the mequinomycin A biosynthesis gene cluster to optimize the heterologous host, and the mequinomycin A acts on peptidoglycan transferase structural domain active sites of bacterial cell walls aPBP to effectively inhibit the synthesis of the cell walls of Gram-positive bacteria. Large-fragment assembly and promoter optimization arecarried out on the mequinomycin biosynthesis gene cluster by means of genetic engineering, and the mequinomycin A resistance gene MaPBP is introduced into the optimized strain II to further improve the mequinomycin resistance of the strain II. Finally, the heterologous expression yield of the mequinomycin A in the strain LX03 is increased by 110% compared with that of the original strain I. According to the invention, not only the genetic engineering modification method of large-segment gene clusters is described, but also a precedent that a Streptomyces. Albus host is optimized by a resistance gene to improve the yield of a target compound is started.

The invention discloses a biological seedling-strengthening agent capable of enhancing stress resistance of rice, and also discloses a preparation method of the seedling-strengthening agent. It belongs to the field of agricultural production technology. The seedling strengthening agent comprises the following raw materials in parts by weight: 5-20 parts of biological bacteria agent, 5-40 parts of distiller's grains, 10-30 parts of urea, 2-10 parts of potassium magnesium sulfate, 6-15 parts of superphosphate, 0.1-1 part of paclobutrazol, 0.1-1 part of naphthaleneacetic acid. The strain of the biological agent is Streptomyces albus, and the preservation number is CGMCC No.15117. The biological seedling strengthening agent of the present invention is safer to use and does not cause phytotoxicity. The low temperature resistance ability of rice can be enhanced, and the normal growth of rice can be prevented from being affected by low temperature. Turn distiller's grains from waste into treasure, use its own acidity to regulate the soil, and secondly use the medium and trace elements contained in it to provide nutrition for rice.

The invention provides a streptomyces albus decomposition accelerator and the application thereof in saline-alkali soil straw decay soil condition improvement. The decomposition accelerator comprises 2.2-3.1 parts of compound microorganism bacterial powder, 36-42 parts of Chinese elm barks, 24-29 parts of rose pollen, and 6-11 parts of scenedesmusarcuatus. The compound microorganism bacterial powder comprises 44*109 CFU / g streptomyces albus, 30*109 CFU / g rhodopseudomonas capsulata, 5*109 CFU / g lactobacillus bulgaricus, and 14*109 CFU / g bacillus subtilis. After the decomposition accelerator is adopted in saline-alkali soil straw decay soil condition improvement for a month, the content of salt in a 0-30 cm soil layer is 0.28-0.31%, the pH value of soil is 7.3-7.6, the mean size of the soil granular structure is 3.0-3.5 mm, and the total porosity of soil is 54-59%.

The invention relates to a strain of streptomyces albus for preventing and controlling aspergillus flavus and aflatoxin and application thereof. Microbes are separated from intestinal tracts of sea cucumbers under an oligotrophic culture condition by using a culture medium only containing agar powder; and in addition, the strain of streptomyces albus is separated from the intestinal tracts of healthy sea cucumbers. After being fermented, the streptomyces albus generates a great number of active secondary metabolites capable of inhibiting the growth of a pathogenic fungus of the aspergillus flavus and the synthesis of the aflatoxin. In addition, the streptomyces albus can completely inhibit the pollution of fungi and fungal toxin in the storage process of grains such as peanuts; and wide application prospects are realized in the field of grain safety.

The invention belongs to the field of biochemical engineering, and relates to an extraction method of polylysine. The method comprises the following steps: 1) centrifuging fermentation liquor of streptomyces albus for producing polylysine, and removing mycelia to obtain supernate; (2) adjusting the pH value of the obtained supernate, continuously centrifuging, and removing protein floccules to obtain clear fermentation liquor; (3) carrying out ion exchange on clarified fermentation liquor obtained by pulping through weakly acidic cation exchange resin D152, and filtering; 4) desorbing the filtered resin to obtain an ion exchange liquid; 5) concentrating the ion exchange liquid, and decolorizing with activated carbon; performing suction filtration with filter paper to remove activated carbon, precipitating filtrate with ethanol, and dissolving and precipitating with a small amount of water; and 6) after the organic solvent is completely volatilized, feeding residues for freeze drying to obtain powder, namely the polylysine. The method is simple and easy to implement, and the purity and the recovery rate of polylysine can be improved.