A kind of biocontrol agent for apple tree rot disease and its application

Abstract

The invention discloses an antibiological inoculant for controlling apple tree canker, and application of the antibiological inoculant. The antibiological inoculant can grow and propagate directly by using pathogenic mycelium as nutrition, and can induce the self to produce multiple extracellular enzyme capable of degrading cell walls of pathogenic bacteria; a streptomyces albidoflavus Actin-1 strain, colonized at disease spots of the apple tree canker for a long time, is activated and subjected to spore production, so that spore suspension is prepared; after that, a fermented seed solution is prepared by continuously carrying out liquid fermentation; finally, the fermented seed solution is inoculated into a solid fermentation medium, and is then fermented, dried in the air, crushed and screened so as to be prepared into spore raw powder; the spore raw powder is then scientifically screened and compounded by using auxiliary agents such as an ultraviolet protective agent, a dispersing agent and a carrier. The antibiological inoculant provided by the invention has the multiple advantages of being good in control effect, high in efficiency, low in recurrence rate and high in environmental adaptability and stability, not easy in producing drug-resistance, and the like, thus being a first choice for biological control; the antibiological inoculant has a great significance for improving the preventing and treating effect of fruit and vegetable pathogenic bacteria such as the apple tree canker, preventing pathogen recurrence and protecting environment.

Description

technical field [0001] The invention relates to a biocontrol bacterium, in particular to a biocontrol bacterium for apple tree rot disease and an application thereof. Background technique [0002] Apple tree rot (Valsa mali Miyabe et Yamada), commonly known as rotten skin disease and stinky skin disease, is an important disease of northern apple trees caused by the genus Valsa ceratosperma. The disease mainly harms fruiting trees that are more than 3 years old, causing tree vigor to weaken, branches to die, dead trees and even garden destruction. The symptoms of the disease are mainly ulcer-based. In the ulcer type, reddish-brown, water-stained, slightly raised, round to oblong lesions appear on the trunk and bark in early spring. The texture is soft, easy to tear, and easy to sag when pressed by hand. In the later stage, it shrinks and sinks, and there are cracks on the edge of the diseased part. There are obvious small black spots, that is, pycnidia. When wet, orange-yel...

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Problems solved by technology

[0014] The disease prevention effect of Streptomyces disclosed above is still not very satisfactory, and the disease prevention mechanism is the same as most bacteria and fungi, mainly through the metabolism to produce antibiotics, antimicrobial peptides and other antibacterial substances to produce antagonistic effects to inhibit the growth and reproduction of pathogenic bacteria, and the disease prevention effect Incomplete, can not cure apple tree rot

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