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Trichoderma koningii, and compound microbial agent composition and application thereof

A compound microbial inoculum and the technology of Trichoderma canning, applied in the field of microorganisms, can solve problems such as low crude protein yield, achieve high cellulase activity, and solve the effect of environmental pollution

Active Publication Date: 2013-09-04
马伟超 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a combination and use of Trichoderma korningii and its composite microbial agent, to solve the defect of low yield of fermentation product crude protein in the prior art

Method used

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  • Trichoderma koningii, and compound microbial agent composition and application thereof
  • Trichoderma koningii, and compound microbial agent composition and application thereof
  • Trichoderma koningii, and compound microbial agent composition and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1, Trichoderma koningeni HK43-5 produces cellulase activity research

[0031] 1.1 Test material

[0032] Raw materials: Potato residue (Qin'an County Yibin Starch Factory), wheat bran (commercially available)

[0033] Strain: Trichoderma koningii (Trichoderma koningii) 40144 (purchased from China Industrial Microbial Culture Collection Center, CICC), mutagenic strain: Trichoderma koningii HK43-5 (obtained from Trichoderma koningii 40144 after space mutagenesis) .

[0034] Seed medium: malt extract 10g, tap water 1000ml, natural pH.

[0035] Potato residue solid fermentation medium: potato residue / bran=9 / 1 (W / W), moisture content 83%, natural pH.

[0036] 1.2 Test method:

[0037] ① Fermentation test

[0038] The spore concentration of Trichoderma koningeni in the logarithmic growth phase was adjusted to 108 spores / ml, and inoculated on the solid-state fermentation medium according to the same inoculum size, and the change of cellulase activity in the med...

Embodiment 2

[0053] Embodiment 2, composite microbial bacterial agent combination

[0054] 2.1 Saccharification bacteria preparation

[0055] Trichoderma koningen HK43-5 and Aspergillus awamori 40499 strains were inoculated into 1% malt extract medium respectively, cultured on a shaking table at 200r / min at 30°C for 60h, centrifuged at 4000r / min for 10min, and the supernatant was removed to obtain wet weight bacteria Then the two are mixed according to the following Table 1 (mass ratio) to obtain the saccharification bacteria preparation, and a protective agent can also be added, mixed, freeze-dried, and packaged. The protective agent can be, one is 10% skim milk and 5% glycerin by mass; the other is 10% skim milk, 5% trehalose and 5% vitamin C by mass.

[0056] Table 1

[0057] Recipe 1 Recipe 2 Recipe 3 Corning Trichoderma HK43-5 2 1 1 Aspergillus awamori 40499 1 1 2

[0058] 2.2 Yeast preparations,

[0059] Candida tropicalis strain 1779 was ino...

Embodiment 3

[0060] Embodiment 3, composite microbial bacterial agent fermentation

[0061] 3.1 Optimization of fermentation conditions

[0062] According to the level of each factor determined by the single factor test, the Plackett-Burman design was used to screen out the factors with significant influence from 8 influencing factors. Each factor takes 2 levels: high level (+1) and low level (-1). As shown in table 2.

[0063] Table 2. The levels and value ranges of each factor in the Plackett-Burman design

[0064]

[0065]

[0066] results and analysis

[0067] 1. The results of the Plackett-Burman design are shown in Table 3.

[0068] Table 3. Plackett-Burman Design Results

[0069]

[0070] The above results were analyzed by variance, and the results are shown in Table 4.

[0071] Table 4. Analysis of Plackett-Burman Design Results

[0072]

[0073]

[0074] Note: * means significant impact, ** means extremely significant impact

[0075] According to the princi...

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Abstract

The invention relates to the technical field of microbes, and specifically relates to trichoderma koningii, and a compound microbial agent composition and an application thereof. The invention discloses trichoderma koningii HK43-5 (CGMCC No7422), a compound microbial agent composition composed of a glycosylated bacterial preparation and a forage yeast preparation which are packaged separately, wherein the glycosylated bacterial preparation comprises the trichoderma koningii HK43-5 (CGMCC No7422). Cellulose produced by the trichoderma koningii HK43-5 (CGMCC No7422) has high activity, can be used as an efficient glycosylated bacterial preparation in production of industrial and agricultural residual protein forage. The compound microbial agent composition can efficiently utilize industrial and agricultural residues, particularly potato residues, to produce single cell protein. Crude protein content in fermented mycoprotein is 50.49% which is far superior to that reported in a conventional technology. The industrial and agricultural residues are changed to wealth; and the produced bacterial protein can be applied in the forage to partly substitute soybean meal or fish meal. At the same time, the environment pollution problems resulted from the industrial and agricultural residues are solved.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a combination and application of Trichoderma koningenii and its composite microbial agent. Background technique [0002] Bacterial protein feed is a kind of biological fermentation protein raw material, which is a microbial protein (or bacterial protein) obtained by using various substrates to cultivate bacteria, yeast, mold, algae and basidiomycetes on a large scale. Bacterial protein, also known as microbial protein or single cell protein (SCP), is an important protein source for the food industry and feed industry. [0003] There are many kinds of raw materials for the production of single-cell protein, which can be roughly divided into three categories. (1) Industrial waste liquid: including papermaking waste liquid, alcohol waste liquid, monosodium glutamate waste liquid, starch waste liquid, citric acid waste liquid, molasses waste liquid, Wood hydrolysis waste liqu...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N1/00C12N1/16A23K1/00A23K1/14C12R1/885C12R1/665C12R1/72C12R1/74
CPCY02P60/87
Inventor 马伟超安建平
Owner 马伟超
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