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Preparation method of straw decomposition agent

A technology of straw decomposing agent and bacterial agent, which is applied in the field of preparation of decomposing agent, can solve the problems of low straw utilization rate and long natural composting time, and achieve the effect of improving soil urease activity and cellulase activity

Inactive Publication Date: 2015-06-10
DALIAN SANKE BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the time required for natural composting is relatively long, and the utilization rate of straw is low. Therefore, it is urgent to develop an efficient microbial compound agent to improve the decomposing speed and decomposing rate of straw.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] A straw decomposing agent, the straw decomposing agent is Bacillus subtilis (Bacillus subtilis) bacterial agent, Aspergillus niger (Aspergillus niger) bacterial agent, plant Lactobacillus (Lactobacillus plantarum) bacterial agent, Trichoderma viride (Trichoderma viride) bacterial agent , Saccharomyces cerevisiae (Saccharomyces cerevisiae) bacterial agent mixed, the specific method is as follows:

[0017] (1) Inoculate each of the bacterial strains on a slant medium commonly used for each strain and cultivate them; wherein Bacillus subtilis is 28-32°C, pH is 7.2-7.4, and the culture time is 2 days; Aspergillus oryzae is 25°C, cultivated The time is 6 days; the culture time of Lactobacillus plantarum is 25℃, the culture time is 2 days; the culture temperature of Trichoderma reesei is 25~28℃, the natural pH is 3 days; the culture time of Saccharomyces cerevisiae is 28~32℃, the pH is 7.2~ 7.4, the culture time is 2 days;

[0018] (2) Select the single colony of each strain...

Embodiment 2

[0027] A straw decomposing agent, the straw decomposing agent is formed by mixing Bacillus subtilis inoculum, Aspergillus oryzae inoculum, Lactobacillus plantarum inoculum, Trichoderma reesei inoculum, and Saccharomyces cerevisiae inoculum. The specific method is as follows:

[0028] (1) Inoculate each of the bacterial strains on a slant medium commonly used for each strain and cultivate them; wherein Bacillus subtilis is 28-32°C, pH is 7.2-7.4, and the culture time is 2 days; Aspergillus oryzae is 25°C, cultivated The time is 6 days; the culture time of Lactobacillus plantarum is 25℃, the culture time is 2 days; the culture temperature of Trichoderma reesei is 25~28℃, the natural pH is 3 days; the culture time of Saccharomyces cerevisiae is 28~32℃, the pH is 7.2~ 7.4, the culture time is 2 days;

[0029] (2) Select the single colony of each strain cultivated in step (1), inoculate them in the common liquid medium for each strain, and culture on a shaking table until the effec...

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Abstract

The invention discloses a preparation method of a straw decomposition agent. The straw decomposition agent is prepared by using bacillus subtilis, aspergillus oryzae, lactobacillus plantarum, trichoderma reesei and saccharomyces cerevisiae as preparation strains, carrying out activation and primary and secondary culture on the strains respectively to obtain various strain solutions with bacterial count of 106-108 / ml and mixing the strain solutions according to a certain proportion. The straw decomposition agent has the beneficial effects that the straw decomposition agent can be used for quickly degrading straws to prepare fertilizers and ensures that the straw decomposition time is at least 15 days ahead of the normal composting fermentation time; organic straw fertilizers prepared by the preparation method are used as base fertilizers, so that the contents of available nitrogen, rapidly available phosphorus, potassium and organic matters in soil are higher than those of available nitrogen, rapidly available phosphorus, potassium and organic matters in the soil in which straws are directly used and fertilizers are not applied; the urease activity and cellulase activity of the soil are obviously improved compared with those of the soil in which straws are directly used and fertilizers are not applied.

Description

technical field [0001] The invention relates to a decomposing agent, in particular to a preparation method of a decomposing agent for degrading straw. Background technique [0002] Crop straw is a huge resource with the characteristics of large quantity, variety and wide distribution. According to statistics, the annual output of straw in my country is about 700 million tons, but the utilization efficiency of straw is extremely low, and most of the straw is incinerated and discarded, causing serious environmental pollution and waste of resources. At present, straw is mostly used in agriculture by returning it directly to the field. However, due to the slow decomposition rate of straw returning to the field in cold soil and the decrease in soil temperature, which affects the growth of crops, the proportion of straw returning to the field has always been relatively low. Returning straw to the field through composting is undoubtedly the best way to solve these problems. By co...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/18C12N1/14C05F11/08C12R1/125C12R1/69C12R1/25C12R1/885C12R1/865
CPCC12N1/20C05F11/00C05F11/08C12N1/14C12N1/18
Inventor 崔国臣崔昌娜顾艳丽周悦
Owner DALIAN SANKE BIO ENG
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