49 results about "Pectinase activity" patented technology

The invention discloses alkaline pectinase PelN, as well as encoded gene and an application of the alkaline pectinase PelN. The PelN is protein formed by an amino acid sequence shown as sequence 1 in a sequence table, and has alkaline pectinase activity, and the preservation number of a recombinant bacterium (escherichia coli) BL21 (DE3) PelN containing the encoded gene of the protein in China General Microbiological Culture Collection Center (CGMCC) is CGMCC No. 7166. Experiments prove that the enzyme activity of the protein PelN for degrading polygalacturonic acid is 4590-4950U/mg; the heat stability is better; the relative enzyme activity is above 90% through heat preservation for 120 minutes at 45 DEG C; the optimum pH value of the alkaline pectinase is 9.8; the optimum temperature is 65 DEG C; and production methods of the protein PelN, the recombinant bacterium and the alkaline pectinase provide efficient paths for industrial large-scale production of the alkaline pectinase.

Method for extracting oils, proteins and fermentable sugars from vegetable material in an aqueous medium, includes:

• • a) adding water to the vegetable material;
  • b) adding an enzyme mixture containing at least one cellulase, at least one hemicellulase, and at least one pectinase, the ratio between the pectinase activity and the cellulase activity being at least 0.14, and the ratio between the pectinase activity and the hemicellulase activity being at least 7.10⁻³, the pectinase activity being less than 120 μmol/min/mL;
  • c) incubating the vegetable material and the enzyme mixture with stirring to release oils, proteins and fermentable sugars in the reaction medium;
  • d) separating the reaction medium to obtain free oil, an aqueous phase containing proteins and fermentable sugars, and a solid phase;
  • e) optionally separating and recycling an emulsion of free oil or aqueous phase, to the medium;
  • f) separating the proteins and fermentable sugars from the aqueous phase.

The invention discloses citrus steamed fish sauce and a preparation method thereof, and belongs to the technical field of food fermentation engineering. According to the citrus steamed fish sauce disclosed by the invention, the technology that soybeans, flour, bran, citrus peels and purple perilla are used as starter propagation raw materials and aspergillus oryzae 3.042 and aspergillus niger 3.350 cooperate for starter propagation is adopted, at the middle stage of fermenting soy sauce mash, saccharomyces rouxii is added, and at the later stage, torulopsis bombicola is added. The aspergillus niger has higher cellulose activity, lipase activity and pectinase activity, and can achieve the enzyme system complementary effect with the aspergillus oryzae 3.042. The activity of the cellulase, the activity of the lipase and the activity of the pectinase obtained at the stage of starter propagation are higher, the cellulase, the lipase and the pectinase are used for decomposing the citrus peels and the purple perilla and promoting the release of various active substances in the citrus skins and the purple perilla. The addition of the two kinds of salt resistant fragrance-producing yeasts at the middle stage and the later stage of fermentation can promote the formation of flavor substances of the steamed fish sauce, improve the flavor of the steamed fish sauce and increase the kinds of the flavor substances.

The invention relates to a complex enzyme capable of degrading a cotton seed hull as well as a ramie induction based preparation method and application thereof. The complex enzyme comprises the components of cellulose, xylanase and pectinase vigour. The preparation method comprises the following steps of: vaccinating a trichoderma reesei seed into a seed culture medium for culturing for 24-48 hours under the conditions of 25-30 DEG C and 120-250r/minute; inoculating to a fermentation culture medium containing 1-10 percent of ramie powder by the inoculum size of 4-10 percent to produce enzymes; further inducing to produce enzymes by adding the ramie powder in the culture process; culturing for 4-8 days under the conditions of 25-30 DEG C and 120-250r/minute; and filtering and centrifuging to obtain rough enzyme liquid. The complex enzyme prepared by the invention has the advantages of higher enzyme activity, safety, environmental protection, simple operation, high controllability, mildreaction condition compared with conventional alkali treatment and favorable application prospect and is more suitable for a complex enzyme system for degrading a cotton seed hull.

The invention relates to a bacterial strain for fermentation of orange peel powder to produce a pectinase preparation and a method of enhancing enzyme activity by a surfactant. In the invention, Aspergillus japonicus PJ01 is employed to produce the pectinase preparation, the activity of the prepared pectinase preparation is represented by pectinase activity, cellulase activity and hemicellulase activity. The production method of the pectinase preparation includes: taking crushed orange peel powder as a sole carbon source, adding the orange peel powder into an inorganic nutrient salt in a Czapek medium, then adding 0.1-0.5%(wt) of PEG4000 into a liquid Aspergillus niger fermentation medium, carrying out shaking cultivation for 3-5 days, thus significantly improving the exo-pectinase activity, the cellulase activity (in terms of CMCase enzyme) and hemicellulase activity (in terms of Xylanase) relative to the control group. This method is simple, can significantly improve the pectinase preparation production ability of Aspergillus, and has the characteristic of environmental friendliness, thereby having good application prospects.

The invention discloses a high-activity composite enzyme for feed, belonging to the technical field of preparation of an enzyme preparation for feed. An Aspergillus niger DM-18 strain for a high-yield composite enzyme for feed, which is used as an initial strain, is subjected to liquid submerged mixed fermentation in an optimized culture medium under specific fermentation conditions by an improve fermentation technique to obtain the composite enzyme for feed, which has the advantages of complete enzymatic system, high enzyme activity, high temperature resistance and wide pH value resistance range. The crude enzyme fermentation liquid of the high-activity composite enzyme for feed contains multiple enzymes, wherein the proteinase activity is 6500-6700 U/ml, the mannase activity is 1500-1700 U/ml, the alpha-galactase activity is 1200-1300 U/ml, and the pectinase activity is 1000-1200 U/ml. When the crude enzyme fermentation liquid is subjected to the heat stability test and pH stability test, the test result indicates that all the enzymes have higher enzyme activity (up to higher than 80%) at 30-75 DEG C under the pH value of 2-7.

The invention relates to the technical field of cultivation of gastrodiaelata, in particular to French Armillariamelleaxiaocaoba No.1 and application thereof in cultivation of the gastrodiaelata. Thepreservation number of the French Armillariamelleaxiaocaoba No.1 is CGMCC No.16781. Experiments prove that the yield and the quality of the gastrodiaelata cultivated by the French Armillariamelleaxiaocaoba No.1 can be improved. The activities of extracellular laccase, extracellular cellulase, extracellular xylan, extracellular pectinase and an extracellular amylase of a new strain of the French ArmillariamelleaXiaocaoba No.1 are significantly higher than those of other strains, the growth rate of the new strain is higher and the production cycle is shortened when the French ArmillariamelleaXiaocaoba No.1 is used for producing the strain. In addition, the polysaccharide content of the Armillariamellea is higher, and the Armillariamelleaxiaocaoba No.1 has a higher medicinal value.

Excess sludge production in a bioreactor of a wastewater treatment plant in which excess sludge is being produced is reduced by adding an activated sludge material having a chitinase specific activity of at least 150 Units/g-MLSS and a pectinase specific activity of at least 120 Units/g-MLSS to the bioreactor. After the addition of the activated sludge material, the bioreactor is controlled by adding the activated sludge material when any one of the chitinase activity, the pectinase activity, and the protease specific of the sludge in the bioreactor drops below the lower limits of 50 Units/L, 40 Units/L, and 0.3 Units/L, respectively.

The invention discloses a method for promoting chlorella growth. The invention discloses application of pectinase and (2,4,6-trihydroxylphenyl)-4-(4-hydroxyphenyl) pyrazole in promoting algae growth. The pectinase is a common enzyme in the food field, and the (2,4,6-trihydroxylphenyl)-4-(4-hydroxyphenyl) pyrazole is a substance capable of promoting pectinase activity, which can avoid influence on other organisms in environment. A using method has the characteristics that the (2,4,6-trihydroxylphenyl)-4-(4-hydroxyphenyl) pyrazole is added to a water body when the chlorella grows to a certain stage, so that a promoting effect is taken when the chlorella just begins to grow to keep algae at a relatively high density and to effectively promote algae growth; the pectinase and the (2,4,6-trihydroxylphenyl)-4-(4-hydroxyphenyl) pyrazole have the features of being safe, efficient, degradable and the like; and the pectinase and the (2,4,6-trihydroxylphenyl)-4-(4-hydroxyphenyl) pyrazole can be extensively applied to a natural or landscape water body.

The present invention provides enriched polynucleotides, and enriched polypeptides having pectinase activity. The present invention also includes methods of using the polynucleotides and polypeptides described herein. For instance, the methods include producing a metabolic product, such as ethanol.

The invention relates to the field of medical dressing materials, and particularly relates to a method of preparing a hemostatic dressing by using hydrogel. When in preparation of the hydrogel, orangepeels are boiled to passivate pectinase activity, pectin is extracted through an acid method, methoxyl is removed through strong base so as to form degreased pectin, then amino and carboxyl in adipoyl dihydrazide and the like are used to perform dehydration-condensation reaction so as to form amido bonds to obtain the hydrogel preventing breaking; a cross-linking agent improves the cross-linkingdensity of molecular chains, medicinal auxiliary materials are absorbed by a user so as to add nutrition to tissues of the wound, increase cell activity, provide energy for cell division activity andimprove the healing speed, the anticoagulant property of sodium heparin can prevent the wound from bonding with the hemostatic dressing, preventing flowing blood from solidifying and allowing the blood to be adsorbed at a faster speed, the cross-linking agent, the medicinal auxiliary materials and the sodium heparin are combined to stop excessive bleeding. The hemostatic dressing prepared by the method has good hemostatic performance, can better stop bleeding for a long time, and has high adsorbability, thereby being a safe and efficient hemostatic dressing and being worth promoting and using.

The invention discloses a preparation method of a high-activity composite enzyme for feed, belonging to the technical field of preparation of an enzyme preparation for feed. An Aspergillus niger DM-18 strain for a high-yield composite enzyme for feed, which is used as an initial strain, is subjected to liquid submerged mixed fermentation in an optimized culture medium under specific fermentation conditions by an improve fermentation technique to obtain the composite enzyme for feed, which has the advantages of complete enzymatic system, high enzyme activity, high temperature resistance and wide pH value resistance range. The prepared crude enzyme fermentation liquid contains multiple enzyme activities, wherein the proteinase activity is 6500-6700 U/ml, the mannase activity is 1500-1700 U/ml, the alpha-galactase activity is 1200-1300 U/ml, and the pectinase activity is 1000-1200 U/ml. The enzymes still have higher enzyme activity (up to 80% or above) at 30-75 DEG C under the pH value of 2-7; and thus, the composite enzyme is more suitable for the demands of a processing technique in feed industry, and the processed feed has low loss of enzyme activity.

The invention belongs to the technical field of biological fermentation engineering and relates to pectinase for wood processing. Brevibacillus agri HS021 with a preservation number CCTCC M2015031 is adopted for obtaining the pectinase for wood processing by means of strain culture and fermentation. The pectinase for wood processing has the advantages that firstly, endophytes which are completely adaptive to internal environments of plants are adopted as original strains for preparation of pectinase solution which is capable of giving full play to pectinase activity in an environment with pH value ranging from 4.5 to 6.0, thereby laying the foundation for implementation and promotion of biological processing of wood materials; secondly, the pectinase with high efficiency in wood processing has a certain promotion function in implementation of biological pulping, green defibration and biological textiles.

The invention belongs to the technical field of tobacco preparation, and particularly relates to a method for reducing the tobacco sheet pectin content through a three-step manner. A specific strain is subjected to fermentation firstly, a crude enzyme solution having maximum pectinase activity is prepared, and the crude enzyme solution is directly used in a tobacco sheet production process by adopting a three-step manner, thus reducing the pectin content of a sheet product. Through optimizing a processing process, an optimum degrading effect is obtained, thus improving tobacco quality. The enzyme activity of the crude enzyme solution prepared by fermentation of the specific strain can be as high as 2986.87 U/mL, and application potential is good. After the crude enzyme solution is further used for tobacco sheet raw material processing, the pectin content of a tobacco sheet is finally reduced. The three-step manner is a manner for performing enzymatic hydrolysis in different processing stages of a tobacco raw material leachate in a tobacco sheet processing process, and the pectinase prepared by fermentation is added separately in different process stages so that the pectin content of a tobacco sheet finished product is reduced.