The invention relates to the technical field of tobacco preparation, and in particular relates to a
pectinase produced through mixed bacterium
fermentation, and an application thereof in tobacco sheet
processing. The
pectinase is obtained through the steps of strain activation, seed liquid preparation,
fermentation culture,
enzyme solution crude extraction, and the like. The crude
enzyme solution is diluted by 0-20 times, and the diluted crude
enzyme solution is used for treating tobacco sheets by enzymolysis for 2-6h at a temperature of 40-50 DEG C according to a ratio that tobacco sheet to
pectinase is 1:(1-5). An overall technical
route of the application comprises the following steps: specific strains are mixed and fermented, wherein an optimal
fermentation condition is obtained by screening and optimization, such that the crude enzyme solution with highest pectinase enzyme activity is obtained; the crude enzyme solution is used for treating tobacco sheets, such that
pectin content in the tobacco sheets can be reduced, and a best degradation effect can be obtained. The maximal enzyme activity of the crude enzyme solution provided by the invention can be higher than 3200U / mL. When the crude enzyme solution is used in tobacco sheet treatment, the
pectin content in the tobacco sheets can be reduced by approximately 1 / 4, and tobacco smoking quality can be well improved.