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Application of bacillus subtilis in degrading pectin in tobacco product

A technology of Bacillus subtilis and tobacco products is applied in the application field of Bacillus subtilis xp strain in degrading pectin in tobacco products, can solve problems such as adverse effects on tobacco safety, incomplete combustion of tobacco leaves, etc., and achieves good application value and Application prospect, effect of reducing pectin content, and simple operation process

Active Publication Date: 2017-05-17
CHINA TOBACCO HENAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

During the smoking process of cigarettes, the pectin in the tobacco leaves will cause incomplete combustion of the tobacco leaves and produce methanol, which is further oxidized into formaldehyde and formic acid, which is the main factor for the irritation of smoke and is also detrimental to the safety of tobacco. influences

Method used

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  • Application of bacillus subtilis in degrading pectin in tobacco product
  • Application of bacillus subtilis in degrading pectin in tobacco product

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Experimental program
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Embodiment

[0024] In order to use the Bacillus subtilis xp strain for degrading pectin in tobacco products, the strain needs to be properly amplified and cultured first, and then it can be used when it is prepared to contain a certain amount of active bacterial agent. The specific preparation method of the bacterial agent is as follows.

[0025] (1) Activation of strains, use the inoculation loop to pick the strains on the slanted medium (the formula is the same as LB liquid medium, only add 15g / L agar powder), place it in LB liquid medium for suspension culture, and cultivate at 30°C for 12h Activation of bacteria;

[0026] (2) Amplification of strains, transfer the activated strains in step (1) to LB liquid culture medium, and incubate at 35°C to OD 600 =3.5 or so, it can be used as a bacterial agent for degrading tobacco products after being evenly suspended.

[0027] When the above-mentioned bacterial agent is specifically used to degrade pectin in tobacco products, it is uniformly ...

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Abstract

The invention belongs to the technical field of tobacco preparation, and particularly relates to an application of bacillus subtilis xp strain in degrading pectin in a tobacco product. The bacillus subtilis is bacillus subtilis xp strain, the strain is preserved at China Center for Type Culture Collection, and a preservation number is CCTCC No: M 2011452; the strain is used for reducing the content of pectin in the tobacco product; and when in use, the bacillus subtilis xp strain is prepared into a microbial inoculum, the microbial inoculum is uniformly sprayed onto the surface of the tobacco product according to a mass ratio of 1.0 percent of the tobacco product, and then the storage and alcoholization are performed for 30 to 40 days at the temperature of 30 to 37 DEG C and the humidity of 55 to 65 percent. By selecting the specific bacillus subtilis strain, the bacillus subtilis is proved to have an effect on effectively reducing the content of pectin in the tobacco product, increasing the tobacco grade, ensuring the alcoholization effect and further accelerating the tobacco leaves alcoholization process.

Description

technical field [0001] The invention belongs to the technical field of tobacco preparation, in particular to the application of a Bacillus subtilis xp strain in degrading pectin in tobacco products. Background technique [0002] Bacillus subtilis is a kind of Bacillus genus, widely distributed in soil and decaying organic matter, and it is easy to multiply in subtilis infusion. The surface of the colony is rough and opaque, dirty white or slightly yellow. It can use protein, various sugars and starch to decompose tryptophan to form indole, which is widely used in genetic research. In addition, Bacillus subtilis itself can synthesize enzymes such as α-amylase, neutral protease, lipase, cellulase, etc., which has good application prospects in degrading complex macromolecular compounds. Therefore, in recent years, Bacillus subtilis has gradually become a A hot spot of attention and research. [0003] Tobacco leaf fermentation is also known as tobacco leaf alcoholization. Its ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A24B15/20C12R1/125
CPCA24B15/20C12N1/205C12R2001/125
Inventor 杨宗灿张展马宇平陈芝飞于建春彭玉富周浩郝辉聂聪
Owner CHINA TOBACCO HENAN IND
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