French Armillariamelleaxiaocaoba No.1 and application thereof in cultivation of gastrodiaelata

A technology of Armillaria armillaria and Gastrodia elata, applied in the field of new strains of Armillaria armillaria, which can solve the problems of production confusion, differences, and rotten hemp of Armillaria armillaria strains

Active Publication Date: 2019-07-09
彝良县天麻产业开发中心
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Different strains of Armillaria armillaria have different effects on the yield and quality of Gastrodia elata, showing significant differences
At present, in the cultivation and production of Gastrodia elata, the production of Armillaria elata strains is relatively chaotic. Random isolation of Armillaria elata strains and direct production without testing have resulted in repeated occurrences of rotten hemp and gastrodia elata yield and quality decline.

Method used

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  • French Armillariamelleaxiaocaoba No.1 and application thereof in cultivation of gastrodiaelata
  • French Armillariamelleaxiaocaoba No.1 and application thereof in cultivation of gastrodiaelata
  • French Armillariamelleaxiaocaoba No.1 and application thereof in cultivation of gastrodiaelata

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The acquisition of embodiment 1 bacterial strain, strain identification

[0023] 1.1 Strain isolation

[0024] Two strains of Armillaria armillaria were isolated from wild Gastrodia elata (gastrodia elata tubers with fungal cords) in Xiaocaoba Town, Yiliang County, Yunnan Province, numbered AM1 and AM4. The specific separation method is as follows:

[0025] Wash the soil on Gastrodia elata with clean water, rinse with sterile water three times after running tap water for 15 minutes, soak in 0.1% mercuric solution for 1-2 minutes, rinse with sterile water for 2-3 times, remove residual disinfectant, and then use sterile Cut out the required part of the tissue with a sterile knife and place it in a sterile petri dish, absorb the surface moisture with sterile filter paper, put it on a petri dish with PDA medium added with chloramphenicol, and start cultivating at a constant temperature of 25°C for 5 days Grow new strands.

[0026] The morphological characteristics of th...

Embodiment 2

[0043] Embodiment 2 Gastrodia elata cultivation test

[0044] This example examines the influence of French Armillaria gallica Xiaocaoba No. 1 (CGMCC No. 16781) on the yield and quality of Gastrodia elata as companion planting. For ease of description, the strain is still coded as AM1.

[0045] Taking Armillaria AM2, AM3, and AM4 described in Example 1 as controls, the above four strains were subjected to the concomitant planting experiment of Gastrodia elata.

[0046] Source of Gastrodia elata planting: the first generation of Gastrodia elata locally produced in Xiaocaoba, Yiliang County; cultivation location: Gastrodia elata demonstration garden in Xiaocaoba, 2010m above sea level, 104°12′2″E, 27°45′21″N. The cultivation method of "three nests" is adopted, that is, the method of placing the cultivation holes at the same time by using tree sticks, different Armillaria strains, and Gastrodia elata seeds, and the cultivation was carried out on March 20, 2017; the cultivation h...

Embodiment 3

[0053] Embodiment 3 Biological properties compare

[0054] This example examines the biological properties of Armillaria gallica Xiaocaoba No. 1 (CGMCC No. 16781). For ease of description, the strain is still coded as AM1.

[0055] Armillaria AM2 described in Example 1 was used as a control.

[0056] The improved solid PDA medium was quantitatively inoculated with young fungal cords of strains AM1 and AM2, cultured in the dark at 25°C, and the experimental data were regularly observed and recorded.

[0057] Determination of growth

[0058] From the fourth day of inoculation, the growth amount of the strain was measured by the cross method, and was measured every day until it was overgrown.

[0059] Extracellular enzyme activity assay

[0060] Preparation of crude enzyme solution

[0061] Fill a 2.0mL centrifuge tube with the culture medium around the mycelium at random with an inoculation needle, centrifuge at 12000r / min for 15min, suck out 1mL of the supernatant and dist...

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Abstract

The invention relates to the technical field of cultivation of gastrodiaelata, in particular to French Armillariamelleaxiaocaoba No.1 and application thereof in cultivation of the gastrodiaelata. Thepreservation number of the French Armillariamelleaxiaocaoba No.1 is CGMCC No.16781. Experiments prove that the yield and the quality of the gastrodiaelata cultivated by the French Armillariamelleaxiaocaoba No.1 can be improved. The activities of extracellular laccase, extracellular cellulase, extracellular xylan, extracellular pectinase and an extracellular amylase of a new strain of the French ArmillariamelleaXiaocaoba No.1 are significantly higher than those of other strains, the growth rate of the new strain is higher and the production cycle is shortened when the French ArmillariamelleaXiaocaoba No.1 is used for producing the strain. In addition, the polysaccharide content of the Armillariamellea is higher, and the Armillariamelleaxiaocaoba No.1 has a higher medicinal value.

Description

technical field [0001] The invention relates to the technical field of Gastrodia elata cultivation, in particular to a new strain of Armillaria armillaria and its application. Background technique [0002] Gastrodia elata Blume (Gastrodia elata Blume) is a perennial symbiotic herb of Orchidaceae, and is a traditional precious Chinese medicine. Gastrodia elata has pharmacological effects such as sedative, analgesic, anticonvulsant, anti-aging, helpful for memory, improving circulation and immune function. The nutritional mode of Gastrodia elata is heterotrophic. As early as 1911, it was discovered that the nutrient source of Gastrodia elata was the digestion and infection of Armillaria mellea. According to the color of the flower, the color of the flower stem, the shape of the tuber, the water content of the tuber, etc., Gastrodia elata is divided into 4 types, namely the original variant - red gastrodia (G.elata Bl.f.elata), green gastrodia (G.elata Bl.f.viridis Makino), b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01H15/00A01G18/40A01G22/25C12R1/645
CPCA01H15/00A01G18/40A01G22/25C12N1/145C12R2001/645
Inventor 王忠巧刘朝斌孙友强徐万雷李才汪唐静
Owner 彝良县天麻产业开发中心
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