65 results about "Aspergillus wentii" patented technology

The invention especially discloses a method for preparing soluble corn peptide from Aspergillus niger fermented maize yellow powder, which belongs to the technical field of food biochemistry. According to the invention, Aspergillus niger is used in the method, main components of a medium comprise maize yellow powder, wheat bran and water, a proper amount of cane sugar and dipotassium hydrogen phosphate are added into the main components, and the steps of centrifugation, pumping filtration, decolouring, ultrafiltration and gel chromatography are carried out to obtain a soluble corn peptide solution. The method has the advantages of a simple process, low cost and a few by-products and enables the obtained corn peptide to have high water-solubility, good security, etc.

A de-bonding enzyme produced by Aspergillus niger HYA4 and its use in flax de-gumming are disclosed. The process is carried out by taking potato glucose agar culturing medium as tapered-land preservative culturing medium, taking solid fermenting culture medium as bran, bean dregs, ammonia sulfate and ammonium nitrate, putting ferment under room temperature, leaching by natural water stilly, filtering to obtain coarse enzyme liquid, diluting, and determining pectase and xylanase activity. It's cheap and non-toxic; has more enzyme yield, complete varieties, shorter production period, various raw material sources, more yielding rate and combing rate and less water-source pollution.

The organic selenium derived from aspergillus oryzae and a preparation method thereof of the present invention belong to the field of food and health product additives. The organic selenium of the present invention uses Aspergillus oryzae as a carrier, and exists in the form of Aspergillus oryzae cells, aqueous solution or dry powder. Its preparation method uses selenium compound as raw material, through the process of preparing selenium-containing medium, inoculating and culturing Aspergillus oryzae, collecting Aspergillus oryzae, washing to remove extracellular selenium, sterilizing or/and drying; the organic aspergillus oryzae cell form is obtained by drying. Selenium, or the organic selenium in the form of an aqueous solution obtained from thalline autolysis, or the organic selenium in the form of dry powder obtained by drying the aqueous solution. Aspergillus oryzae has the characteristics of fast growth and high content of organic selenium in selenium-enriched Aspergillus oryzae cells. During the fermentation process, Aspergillus oryzae can convert inorganic selenium into organic selenium, which is easily metabolized and absorbed by the human body. Using Aspergillus oryzae containing organic selenium instead of common Aspergillus oryzae as an additive in food and health products can supplement selenium in a small amount and continuously, and effectively prevent and treat diseases caused by selenium deficiency.

The invention relates to a method for preparing compost by washing wool junk microbial fermentation. Microorganism accounting for 10 percent of the total mass of silt is implanted in the washing wool silt and fermented for 15-30 days at the temperature of 45-65 DEG C. The microorganisms are the composite of bacteria, fungi and actinomycetes, and the number ratio of the bacteria, the fungi and the actinomycetes is 10-30:0.5-2.5:0-1.5. The bacteria are bacillus subtilis, Pseudomonas aeruginosa and Bacillus mucilaginosus with the number ratio of 1-5:0.5-3:0.3-1.5; and the fungi are Aspergillus niger and Penicillium simplicissimum with the number ratio of 1-3:0.5-1.5. The compost produced by fermentation contains 30-80% of organic matter, 3-15% of total nitrogen, 2-10% of total phosphorus and 1-7% of total potassium.

The invention discloses a preparation method of beta-glucosaccharase. The method includes the following steps: selecting Aspergillus niger as parent strain through screening, inoculating the Aspergillus niger, the inoculum size of which is 106-108cfu/g, to bran, rice bran and ammonia chloride, wherein in a solid culture medium prepared from the bran, the rice bran and the ammonia chloride according to the weight percentage of 90-100 percent: 0-10 percent:1-3 percent, the initial water content of the culture medium is 65-75 percent, and the pH is 5.5-6.5, culturing for 48-80h at the temperature of 25-30 DEG C and detecting the activity of beta-glucosaccharase to reach 300-430U/g. The method adopts solid fermentation, uses cheap and rich subsidiary agricultural products as fermentation substrate, simultaneously optimizes the culture medium and culture conditions and achieves high yield of the beta-glucosaccharase under solid fermentation conditions, thus providing scientific basis to the industrialization development of the beta-glucosaccharase.

The invention discloses a preparation process of broad bean paste fermented by mixed aspergillus oryzae and lactobacillus plantarum. The broad bean paste is prepared from the raw materials by weight as follows: 40-80 parts of broad beans, 12-20 parts of edible salt, 15-25 parts of flour, 0.01-0.02 parts of aspergillus oryzae powder, 0.002-0.008 parts of lactobacillus plantarum powder and 40-80 parts of water. The mixed aspergillus oryzae and lactobacillus plantarum is adopted for fermentation, the viscosity of the broad bean paste is improved, so that quality of each batch of the broad bean paste keeps uniform, the risk of pollution of a product by aflatoxin is reduced, and the safety edibility is significantly increased; meanwhile, a temperature control and humidity control technology is adopted, during koji making, the temperature is controlled at 28-32 DEG C, the humidity is controlled to be 75%-80%, the reproduction rate of dominant fermentation strains, namely, the aspergillus oryzae and the lactobacillus plantarum, is increased, koji making time is shortened to 24 hours from traditional 3-5 days, and production cost is saved.

The invention discloses an aspergillus niger gene knockout method and belongs to the technical field of biotechnology. A shearing element and a gene knockout expression cassette are integrated in an aspergillus niger genome simultaneously, resistance genes are induced to be eliminated by inducible promoters later, and accordingly, resistance can be eliminated by transforming aspergillus niger once. The method can be used for knocking out multiple genes continuously; an effective method and a new concept are provided for metabolic transformation of aspergillus niger.

The invention relates to aspergillus oryzae ZA210. The aspergillus oryzae has the advantages of thick and solid hypha, high germination rate, low raw material consumption rate, and high comprehensiveenzyme activity, so that the fermented raw oil has the advantages of high total nitrogen index, high glutamic acid and alanine content, low arginine content and the like. The aspergillus oryzae is applied to soy sauce production so as to ferment the raw oil with pure flavor, gentle and harmonized taste, outstanding freshness and sweetness, abundant taste, so that the zero-addition pure brewing soysauce can be industrially produced easily, and energy conservation and efficiency increment are realized.

The invention relates to a large-scale culture method of lactobacillus acidophilus. The method is characterized by comprising the steps as follows: 1) preparation of a strain: the strain consists of aspergillus niger spores and lactobacillus acidophilus in the weight ratio being (8-9):1; 2) preparation of a culture medium: the culture medium consists of rice bran, soybean meal, NaCl and wheat bran, and all substance components are mixed and stirred uniformly; 3) solid fermentation: the culture medium is poured in a solid fermentation tank, the solid fermentation tank is inoculated with the aspergillus niger spores, the relative humidity and the air supply are kept unchanged, and culture is performed for 48 h. The method realizes industrial solid fermentation and low-cost and large-scale culture and has the benefits of low cost and high production efficiency.

The invention provides a culture method of Aspergillus niger seeds. The transplantation opportunity is selected by monitoring the acid production speed, the glucose consumption speed or the glucoamylase activity in the culture process. The invention specifically discloses a method for determining the acid production speed, the glucose consumption speed or the glucoamylase activity and discloses influence of the concentration of dissolved oxygen on the culture process. The defect that the growth state of the seeds is judged mainly by observing the sizes and shapes of Aspergillus niger balls in a conventional culture method is overcome. The standard deviation of fermentation results of the seeds cultured with the method is smaller, the seed fermentation in adjacent batches is more stable, the fermentation conversion rate is increased, and the fermentation period is remarkably shortened.

The invention discloses a method for producing chymosin by aspergillus niger solid fermentation. The method adopts a solid basal medium and comprises the following steps of adding a nitrogen source and an acidic nutrient solution containing one or more inorganic salts and an amino acid into the solid basal medium to wet it, mixing well, carrying out a sterilization process at a temperature of 121 DEG C for 30 minutes, cooling, inoculating the sterilized solid basal medium with an aspergillus niger strain, carrying out solid fermentation at a fermentation temperature of 35 to 37 DEG C for 72 to 100 hours, carrying out solid-liquid extraction through adopting water as a solvent, carrying out centrifugation and extraction filtration, and carrying out the processes of vacuum rotation, evaporation, concentration and freeze-drying on supernate obtained by the previous step to obtain crude dry powder of chymosin. Chymosin which has the advantages of low added amount in raw milk such as milk and the like, rapid curd speed, specific catalytic site and low capacity for non-specific protein hydrolysis is produced through the method.

The invention discloses a method for producing potassium gluconate through fermentation of Aspergillus niger, which comprises the following steps: performing one-step jet on starch and starch-containing materials, and performing double-enzyme glucose preparation; using an Aspergillus niger P1205X21 strain which is resistant to high-concentration K<+> to ferment the glucose solution, and feeding an ion membrane KOH solution in the fermentation process to obtain a potassium gluconate solution; and by using a thermal crystallization process of multiple-effect continuous evaporation and crystallization, directly evaporating and crystallizing in a multiple-effect evaporation crystallizer to obtain the potassium gluconate finished product. The invention has the advantages that the raw materials are rich and accessible, the cost is low, the fermentation product is simplex, the product quality is high and easy to control, no waste water and waste gas are generated, no calcium gluconate mother liquor and calcium sulfate waste are generated, the environmental pollution is greatly reduced, the process route is short, evaporation, crystallization, drying and other procedures during calcium gluconate preparation are reduced, vapor is saved, the power consumption is reduced, and the production cost is greatly lowered.

The invention relates to the technical field of pharmaceutical chemistry and microbial medicines, and in particular relates to a norditerpenoid compound obtained from a fermentation product of Aspergillus wentii, a separation and purification method for the norditerpenoid compound and application of the norditerpenoid compound in the aspect of inhibiting the activity of aquatic disease bacteria. The norditerpenoid compound comprises a compound 1 and a compound 2 as shown in the formula I; the compound 1 and the compound 2 are a pair of isomers; the molecular formula of each of the compound 1 and the compound 2 is C19H26O3; the bacteriostatic activity tests show that the compound 1 and the compound 2 have outstanding inhibitory activity on the aquatic disease bacteria such as Edvardsiella tarda, Vibrio harveyi and Vibrio parahaemolyticus, and the minimum inhibitory concentration (MIC) of each of the compound 1 and the compound 2 is 8.0 microgram/mL, and the norditerpenoid compound is expected to be developed into a novel aquatic disease bacteria prevention and treatment drug. The formula I is shown in the description.

The invnetion provides aspergillus fumigatus strain TMS-26 for producing taxol. The strain can produce the taxol under the condition of liquid fermentation. The strain is collected in China Center For Type Culture Collection (Wuhan University) on June 15, 2014, and the collection number is CCTCC M2014258. The taxol yield of the strain under the conditions of PDA liquid culture medium, 28 DEG C, 60r/min and dark culture for 10 to 12d can reach 696.24[mu]g/L to the maximum degree. The novel strain provided by the invention provides good study basis for the breeding of good strains in industrial large-scale production, and the application prospects are wide.

The invention discloses a method for improving yield of resveratrol through fermentation of aspergillus niger. An aspergillus niger strain is cultured in a triangular flask culture medium for 48h, 2% by mass of hypha is evenly added to giant knotweed rhizomes with humidity of 2:1, and the stacking fermentation is carried out at a constant temperature of 40 DEG C for 30h. Through a solid stacking fermentation method, polydatin in giant knotweed rhizomes is converted into resveratrol, and the solid fermentation condition is optimized. In the method, a strong acid-base reaction is not generated, and glycoside does not need to be crudely extracted; meanwhile, expensive equipment does not need to be added, and the method is easy to popularize. The extraction yield of resveratrol is increased to 1.197% and is increased by 30% compared with that of the existing method, so the very obvious technical effects are achieved.