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Preparation method of beta-glucosaccharase

A technology of glucosidase and seeds, which is applied in the field of preparation of β-glucosidase, can solve the problems of soybean isoflavone resource animal body utilization and waste of resources, and achieve the goal of improving absorption and utilization rate, low product cost, and improving economic benefits Effect

Inactive Publication Date: 2010-05-12
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It must be hydrolyzed into aglycone by microorganisms before it can be absorbed and enter the peripheral circulation. In this way, most soybean isoflavone resources cannot be well utilized by passive objects, resulting in a waste of resources

Method used

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Effect test

preparation example Construction

[0014] The preparation method of β-glucosidase of the present invention comprises the following steps:

[0015] 1. Preparation medium: including,

[0016] 1. Prepare slant seed medium: add peeled potato, sucrose and agar into water, and sterilize at 121°C for 20 minutes to obtain slant seed medium, wherein the content of peeled potato is 200g / l, the content of sucrose is 20g / l, and the content of agar 20g / l;

[0017] 2. Prepare solid seed culture medium: add bran into water according to the ratio of 1:1 by weight, stir evenly, and sterilize at 121° C. for 30 minutes to obtain solid seed culture medium;

[0018] 3. Prepare solid production medium: mix bran, rice bran and ammonium chloride at a ratio of 90-100% by weight: 0-10%: 1-3%, and sterilize at 121°C for 30 minutes to obtain a solid production medium .

[0019] 2. Preparation of slant seeds: inoculate the Aspergillus niger strain on the slant seed medium of step 1, and after cultivating at a constant temperature of 28°...

Embodiment 1

[0025] (1) Preparation of medium: including,

[0026] ① Inclined seed medium: peeled potato 200g / l, sucrose 20g / l, agar 20g / l, sterilized at 121°C for 20min;

[0027] ②Solid seed medium: Add 10g of bran to a 250ml Erlenmeyer flask, add tap water at a ratio of 1:1 to the weight of bran, stir evenly, and sterilize at 121°C for 30 minutes;

[0028] 3. solid production medium: 10g of solid medium prepared by the ratio of bran, rice bran and ammonium chloride in a 250ml Erlenmeyer flask prepared by the ratio of 90% by weight: 9%: 1%, sterilized at 121°C for 30min;

[0029] (2) Preparation of slant seeds: inoculate the Aspergillus niger strain on the slant seed medium of step (1) ①, cultivate at a constant temperature of 28°C for 5 days, store in a refrigerator at 4°C, and set aside;

[0030] (3) Seed preparation: place the slanted seeds of step (2) in the solid seed medium of step (1) ②, and cultivate them at a constant temperature at 28° C. for 72 hours;

[0031] (4) Fermentatio...

Embodiment 2

[0033] (1) Preparation of medium: including,

[0034] ① Inclined seed medium: peeled potato 200g / l, sucrose 20g / l, agar 20g / l, sterilized at 121°C for 20min;

[0035] ②Solid seed medium: Add 10g of bran to a 250ml Erlenmeyer flask, add tap water at a ratio of 1:1 to the weight of bran, stir evenly, and sterilize at 121°C for 30 minutes;

[0036] 3. solid production medium: put bran, rice bran and ammonium chloride into a 250ml Erlenmeyer flask, 10g of solid medium prepared in a ratio of 95% by weight: 4%: 1%, sterilized at 121°C for 30min;

[0037] (2) Preparation of slant seeds: inoculate the Aspergillus niger strain on the slant seed medium of step (1) ①, cultivate at a constant temperature of 28°C for 5 days, store in a refrigerator at 4°C, and set aside;

[0038] (3) Seed preparation: place the slanted seeds of step (2) in the solid seed medium of step (1) ②, and cultivate them at a constant temperature at 28° C. for 72 hours;

[0039] (4) Fermentation production: the se...

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Abstract

The invention discloses a preparation method of beta-glucosaccharase. The method includes the following steps: selecting Aspergillus niger as parent strain through screening, inoculating the Aspergillus niger, the inoculum size of which is 106-108cfu / g, to bran, rice bran and ammonia chloride, wherein in a solid culture medium prepared from the bran, the rice bran and the ammonia chloride according to the weight percentage of 90-100 percent: 0-10 percent:1-3 percent, the initial water content of the culture medium is 65-75 percent, and the pH is 5.5-6.5, culturing for 48-80h at the temperature of 25-30 DEG C and detecting the activity of beta-glucosaccharase to reach 300-430U / g. The method adopts solid fermentation, uses cheap and rich subsidiary agricultural products as fermentation substrate, simultaneously optimizes the culture medium and culture conditions and achieves high yield of the beta-glucosaccharase under solid fermentation conditions, thus providing scientific basis to the industrialization development of the beta-glucosaccharase.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation and enzyme engineering, in particular to a preparation method of β-glucosidase. Background technique [0002] Soybean is the main protein feed for livestock and poultry, and it occupies a large proportion in feed ingredients. Soybean is rich in soybean isoflavones. Initially, people's research on soybean isoflavones was aimed at the human body. It was found that soybean isoflavones in humans have obvious curative effects on the prevention and treatment of cancer, cardiovascular disease, and osteoporosis in elderly women. In recent years, research on soybean isoflavones has begun to be carried out on animals. A large number of animal experiments have shown that soybean isoflavones can promote animal growth, enhance body immunity, improve animal lactation performance, increase egg production, improve reproductive function, and promote animal nutrition. Various nutritional and physiol...

Claims

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Application Information

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IPC IPC(8): C12N9/42A23K1/165C12R1/685A23K20/189
Inventor 钱利纯孙建义
Owner ZHEJIANG UNIV
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