32results about How to "Excellent strain" patented technology

The invention discloses a construction method of a saccharomyces cerevisiae strain with a high yield of isobutanol and relates to preparation of microorganism by a recombinant DNA (Deoxyribonucleic Acid) technology. According to the method, the saccharomyces cerevisiae strain with the high yield of isobutanol is constructed by reforming saccharomyces cerevisiae cells by using molecular biology and the gene recombination technology. The method comprises the following steps: S1, constructing plasmids of over-expressing ILV2 (Induced Leukemia Virus), ILV3, ILV5 and ZWF1 genes of saccharomyces cerevisiae; and S2, constructing a saccharomyces cerevisiae strain HBGDAL-103 with the high yield of isobutanol. The method disclosed by the invention overcomes the deficiency that further improvement of the yield of isobutanol is restricted by factors such as imbalance of a coenzyme NADPH / NADP<+> (Reduced Form of Nicotinamide-Adenine Dinucleotide Phosphate) / (Nicotinamide-Adenine Dinucleotide Phosphate<+>) from pyruvic acid to 2-one isovaleric acid due to over-expressed ILV2, ILV3 and ILV5 in the saccharomyces cerevisiae cells of yielding isobutanol in the prior art.

The invention belongs to the field of edible mushrooms and discloses a novel pholiota nameko mushroom block, a mushroom block production process and a special device of production of the mushroom block. The pholiota nameko mushroom block comprises a strain, a cultivation material and a mushroom bag with a breathable film; the production process of the mushroom block comprises the steps of bagging, pressing blocks, sterilizing, inoculating, culturing; the production of the mushroom block is realized by a special device which is block pressing machine; the mushroom block and the production process of the mushroom block are low in pollution rate; through an automatic production device, the production efficiency is improved and the production cost is reduced; meanwhile, the yearly culture times are increased; a factory is positioned in an artificial climate chamber, mycelia are cultured; the mushroom blocks capable of directly producing mushrooms are delivered to mushroom growers; after the mushroom growers cultivate the mushrooms, the mushrooms are changed from being harvested for one time in a year to being harvested for 2-4 times in a year.

The present invention relates to a genetically engineered bacterial strain of Aspergillus niger with high organic acid production under low dissolved oxygen conditions. The construction steps of the genetically engineered bacterial strain are as follows: step 1, constructing a heterologously expressed vhb gene plasmid; the gene vhb sequence The fragment is controlled by the Aspergillus niger 3-glycerol phosphate dehydrogenase gene promoter PgpdA; step 2, the acquisition of the heterologous expression vhb gene strain, namely the Aspergillus niger (Aspergillus niger) genetically engineered strain with high organic acid production under low dissolved oxygen conditions. The present invention is based on the natural characteristics of Aspergillus niger to produce organic acids, and through genetic recombination to transform the physiological characteristics of Aspergillus niger, a genetically engineered strain of Aspergillus niger is obtained. Experiments have confirmed that the genetically engineered strain of Aspergillus niger can produce organic acids under low dissolved oxygen conditions. The acid ability is significantly improved, which provides excellent strains for the preparation of organic acids by microbial fermentation.

The invention discloses Pseudomonas nitroreducens and application thereof, belonging to the field of environmental microorganism. The Pseudomonas nitroreducens CQ1 of the invention is derived from the sewage of oxidation ponds in the sewage treatment system of Beijing Yanshan Petrochemical Co., Ltd., and obtained by enriching, separating and purifying. The Pseudomonas nitroreducens CQ1 is cultured and collected in the China General Microbiological Culture Collection Center (hereinafter referred to as CGMCC) with the collection number being CGMCC No.3159. The invention further discloses the application of Pseudomonas nitroreducens in the biodegradation of quinoline or derivatives thereof. Quinoline or derivatives thereof with the concentration reaching 700mg / L can be degraded by Pseudomonas nitroreducens of the invention, and the degradability can reach 100% after treating for 72h. Moreover, the Pseudomonas nitroreducens CQ1 of the invention is sensitive to kanamycin sulfate, tetracycline and gentamicin and does not carry plasmids.