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Method for producing gamma-linolenic acid protein by biofermentation

A bio-fermentation and linolenic acid technology, which is applied in the field of γ-linolenic acid protein preparation, can solve the problems of long growth cycle, waste of land resources, and limitation of raw material sources, and achieve advanced and scientific technology, lower price and cost, and mature technology and technology Effect

Inactive Publication Date: 2005-02-16
SHENGJIE BIOLOGICAL SCI TECH CHANGCHUN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the limited sources of wild amaranthus and the long growth cycle of artificial planting, a large amount of land resources are consumed, and the source of raw materials is limited, which is far from meeting the growing market demand.
Therefore, people began to search for a new source of gamma-linolenic acid and produce gamma-linolenic acid by microbial fermentation. Has not formed an industrial production scale

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] a) Activation of the original strain

[0055] The original strains were made of silvery mildew Xiaogram provided by Beijing Microbiological Culture Collection Center, which was coded as MW7 after mutagenesis. The original strains were inoculated on the PDA slant medium, the culture temperature was 25-28°C, and the culture time was 96- 120 hours;

[0056] b) Expansion of cultivation

[0057] Transfer the activated strains to the PDA medium of the Kirschner flask for cultivation, the cultivation temperature is 25-28°C, and the cultivation time is 96-120 hours;

[0058] c) shaking flask culture

[0059] With the well-activated and expanded bacterial classification, add 50 milliliters of sterile water to every bottle, scrape off the spores, and make a spore liquid, and the shaking flask culture medium is a seed culture medium, and the 750 milliliter bottle has a capacity of 250 milliliters. After sterilization, Each shaker flask was inoculated with 5 milliliters of spore...

Embodiment 2

[0071] a) Activation of the original strain

[0072] The original strains were made of silvery mildew Xiaogram provided by Beijing Microbiological Culture Collection Center, which was coded as MW7 after mutagenesis. The original strains were inoculated on the PDA slant medium, the culture temperature was 25-28°C, and the culture time was 96- 120 hours;

[0073] b) Expansion of cultivation

[0074] Transfer the activated strains to the PDA medium of the Kirschner flask for cultivation, the cultivation temperature is 25-28°C, and the cultivation time is 96-120 hours;

[0075] c) shaking flask culture

[0076] With the well-activated and expanded bacterial classification, add 50 milliliters of sterile water to every bottle, scrape off the spores, and make a spore liquid, and the shaking flask culture medium is a seed culture medium, and the 750 milliliter bottle has a capacity of 250 milliliters. After sterilization, Each shaker flask was inoculated with 5 milliliters of spore...

Embodiment 3

[0088] a) Activation of the original strain

[0089] The original strains were made of silvery mildew Xiaogram provided by Beijing Microbiological Culture Collection Center, which was coded as MW7 after mutagenesis. The original strains were inoculated on the PDA slant medium, the culture temperature was 25-28°C, and the culture time was 96- 120 hours;

[0090] b) Expansion of cultivation

[0091] Transfer the activated strains to the PDA medium of the Kirschner flask for cultivation, the cultivation temperature is 25-28°C, and the cultivation time is 96-120 hours;

[0092] c) shaking flask culture

[0093] With the well-activated and expanded bacterial classification, add 50 milliliters of sterile water to every bottle, scrape off the spores, and make a spore liquid, and the shaking flask culture medium is a seed culture medium, and the 750 milliliter bottle has a capacity of 250 milliliters. After sterilization, Each shaker flask was inoculated with 5 milliliters of spore...

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Abstract

The invention is a kind of craft for produce gamma-linolenic acid albumen. It contains approaches, such as activation and cultivation of original mushroom, rock and shock culure, first level fermentation, second level fermentation, third level fermentation, dehydration and drying. The mushroom is better, output is great, dry thallus yield is 3.5-4.1%, aliphatic acid is 25-39%, linolenic acid is 20-24% and the cost is low. The cost of raw material can be reduced by 50-80%. The craft is scientific and maturity, so it is able to manufacture in large scale of industrialization and can reach the 3-4 level of 10-30T.

Description

Technical field: [0001] The invention relates to a method for preparing gamma-linolenic acid protein, in particular to a method for producing gamma-linolenic acid protein by fermentation of microorganisms. Background technique: [0002] Diseases are the main factors that endanger human life and health, especially the high incidence of cardiovascular and cerebrovascular diseases, which are more serious. There are more than 100 million people in my country suffering from various degrees of cardiovascular and cerebrovascular diseases. Diabetes is also a very serious common disease, and there are as many as 30 million people in my country suffering from this disease. [0003] γ-Linolenic acid (γ-Linolenic and acid GLA) is a high-level polyunsaturated acid, which belongs to arachidonic acid substances (including various prostaglandins, thromboxanes and leukotrienes, etc.), and is a cellular substance that mediates the activities of cells themselves , they participate in the regu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/15C12P21/00C12P21/02
Inventor 王玉兰刘丹
Owner SHENGJIE BIOLOGICAL SCI TECH CHANGCHUN
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