Industrial green production process of coriolus versicolor glycopeptide

Abstract

The invention discloses an industrial green production process of coriolus versicolor glycopeptide. The industrial green production process comprises the following steps of: cultivating, rejuvenating and screening strains by adopting a low-temperature freezing resuscitation method; cultivating by adopting a reasonable fermentation culture medium in a mixing ratio, wherein the extraction of fermentation liquor comprises the steps of extracting coriolus versicolor polysaccharide from solid-phase mycelium and extracting coriolus versicolor polysaccharide from a liquid phase; dividing the liquid phase into two parts by a reverse osmosis membrane; concentrating and performing alcohol precipitation on one part with the molecular weight of the liquid phase being more than 30,000 to obtain a medicine-grade finished product, treating the other part with the molecular weight of the liquid phase being 30,000 and below 30,000 to obtain a food-grade or feed-grade additive, and reasonably utilizing all the formed graded products. The industrial green production process disclosed by the invention is advanced in technology; by adopting the green production process, the production cost is reduced; on the premise of ensuring that the medicine-grade yield of the original process flow is not reduced, the produced coriolus versicolor powder is added as a functional food base material, a feed additive and feed protein powder; the application of the feed-grade additive fills the blank of the product in China; the industrial green production process disclosed by the invention realizes comprehensive utilization of resources, reduces emission and is suitable for industrial production.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an industrialized green production process of versicolor glycopeptide. Background technique [0002] Coriolus versicolor is the dry fruiting body of Polyporaceae fungus Coriohus versicolor (L.ex Fr.)]. Coriolus versicolor is also known as Versicolor Versicolor Versicolor Versicolor Versicolor Versicolor Versicolor Versicolor Versicolor, Huang Yunzhi, Gray Mushroom, Multi-colored Boletus, Red See Hand, Melaleuca Versicolor Versicolor Versicolor. Born in various broad-leaved trees (willow, poplar, birch, plum, peach, etc.) on stakes, fallen logs and branches, occasionally on pine trunks, distributed in various provinces and autonomous regions of the country, mainly in Northeast China. Recorded in the "Chinese Pharmacopoeia", it has the effects of invigorating the spleen and dampness, clearing heat and detoxifying, and is used to treat diseases such as damp-heat jaundice, rib pain, ano...

AI Technical Summary

Problems solved by technology

Extracting Yunzhi glycopeptide from mycelium by this method not only requires a large investment in equipment, but also the production cost of fermentation mycelium itself is relatively high. Analyzing from the input-output method, per ton of Yunzhi fermented mash is only One kilogram of Yunzhi glycopeptide is obtained, and the rest is discharge. After the 1990s, after innovation and reform, the fermentation broth after solid-liquid phase separation was used to extract exopolysaccharides. The yield has doubled, but it has not yet been resolved. The problem of massive emissions

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