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Pleocidin engineering bacteria capable of enhancing oxygen absorptive capacity, and construction method and fermentation method thereof

A spinosyn and engineering bacteria technology, applied in microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of low spinosyn biosynthesis, sticky bacterial fermentation broth, and insufficient oxygen supply. , to achieve the effect of improving absorption performance, promoting biosynthesis, and enhancing oxygen absorption capacity

Active Publication Date: 2013-07-17
湖南鸿健生物科技有限公司
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Problems solved by technology

[0004] However, the spinosad biosynthesis of wild-type S. spinosa is very low, and the genetic improvement of strains to increase its production has become a current research hotspot. Traditional physical and chemical mutagenesis combined with high-throughput screening has been used. Protoplasts Related reports on the genetic improvement of strains by methods such as regeneration, intergenus fusion of protoplasts, and doubling of genes related to spinosad biosynthesis
During the fermentation process of Saccharopolyspora spinosa, the bacteria grow in filamentous form, and the biosynthesis of spinosad requires fatty acids as precursors. A certain amount of oil is often added in fermentation production, resulting in very viscous fermentation broth and insufficient oxygen supply. Become one of the main factors limiting spinosad biosynthesis

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  • Pleocidin engineering bacteria capable of enhancing oxygen absorptive capacity, and construction method and fermentation method thereof
  • Pleocidin engineering bacteria capable of enhancing oxygen absorptive capacity, and construction method and fermentation method thereof
  • Pleocidin engineering bacteria capable of enhancing oxygen absorptive capacity, and construction method and fermentation method thereof

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Embodiment Construction

[0042] The spinosyn engineering strain of the present invention uses gene recombination technology to convert the hemoglobin gene of Vitiligo hyaline ( vgb ) into the recombinant spinosad engineering bacteria constructed by the S. spinosa genome, and its taxonomic name is the actinomycete S. spinosa S078-1011 ( Saccharopolyspora spinosa S078-1011), was deposited in China Center for Type Culture Collection (CCTCC for short; address: Wuhan University, Luojia Mountain, Wuhan City, Hubei Province) on November 18, 2011, and the preservation number: CCTCC NO: M2011400.

[0043] The present invention will be described in further detail below in conjunction with the accompanying drawings and embodiments.

[0044] The present invention uses the Saccharopolyspora rubrum genome as a template, and designs a pair of primers P1 / P2 according to the sequence published on GenBank (gi: 152688), wherein the 5' end of P1 introduces EcoR Ⅰ Restriction site, P2 introduces mutation site CAGCAC, ...

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Abstract

The invention relates to pleocidin engineering bacteria capable of enhancing oxygen absorptive capacity, and a construction method and a fermentation method thereof. The pleocidin engineering bacteria are gene recombinant pleocidin engineering bacteria constructed by integrating vitreoscilla hemoglobin gene (vgb) into saccharopolyspora genome, wherein the class name is actinomycetes saccharopolyspora S078-1011; and the collection number is CCTCC NO: 2011400. The invention further comprises a construction method for the pleocidin engineering bacteria and a fermentation method for producing pleocidin by using the pleocidin engineering bacteria. The actinomycetes saccharopolyspora S078-1011 can improve the dissolved oxygen absorptive capacity of the bacteria and can obviously promote biological synthesis of the pleocidin under the conditions of normal dissolved oxygen and limited oxygen.

Description

technical field [0001] The present invention relates to a genetically recombinant spinosad engineering bacterium actinomycete Saccharopolyspora spinosa and its construction and fermentation method, in particular to a genetically recombinant spinosad engineering bacterium capable of enhancing oxygen absorption capacity and its construction and fermentation method. Background technique [0002] Chemical pesticides have been used for more than half a century and have brought huge economic benefits to agriculture. However, the abuse of chemical pesticides in the cultivation of crops has caused the increasing resistance of harmful insects, and the effect of pesticides on various pests has been significantly reduced. At the same time, the residual amount is large, threatening human health and causing environmental pollution. Biopesticide or green pesticide is an inevitable trend in the development of pesticides in the future, and it is also a basic guarantee for the sustainable d...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/21C12R1/01
Inventor 夏立秋丁学知罗玉双胡胜标
Owner 湖南鸿健生物科技有限公司
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