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Skin anti-ageing composition

A composition and skin technology, applied in the directions of skin care preparations, cosmetics, cosmetic preparations, etc., can solve the problems of complex ingredients and cannot be used for skin care, etc., and achieve the effects of high safety, elimination of adverse reactions, and lightening of skin spots

Inactive Publication Date: 2019-01-04
灏灵赛奥(天津)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The composition of the invention is complicated, it contains blood plasma, and it is an injection, which needs to be operated by professionals and cannot be used for daily skin care at home

Method used

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  • Skin anti-ageing composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1: Preparation of secreted fluid of mesenchymal stem cells

[0042] 1. Raw materials

[0043] 1. Umbilical cord mesenchymal stem cells:

[0044] 2. Serum-free stem cell culture medium: LONZA, the product name is UltraCULTURE, Cat.12-725F,

[0045] No serum additives: PALL, product name Ultroser G

[0046] Second, the method

[0047] After the serum-free supplement was dissolved in 20ml of serum-free stem cell culture medium, it was added to the serum-free stem cell culture medium at a ratio of 1:100, mixed well for later use, and stored at 4°C.

[0048] Selected umbilical cord mesenchymal stem cells were washed with normal saline and inoculated into T-225 culture flasks (inoculation density 3×10 6 ), add 20ml of serum-free stem cell culture medium (containing 1% Ultroser G), 37°C, 5% CO 2 The next day, all the new serum-free stem cell culture medium (containing 1% Ultroser G) was replaced with 30ml, and the culture supernatant was collected when the cell co...

Embodiment 2

[0053] Example 2: Skin anti-aging composition

[0054] 1. Raw materials:

[0055] 1. Hyaluronic acid: Bloomage Freda Bio-Pharmaceutical Co., Ltd.

[0056] 2. Hydrolyzed Collagen: Guangzhou Zhufeng Import and Export Trading Company

[0057] 3. Mesenchymal stem cell secretion: the mesenchymal stem cell secretion obtained in Example 1

[0058] 4. EGF: Thermo Fisher Scientific (China) Co., Ltd.

[0059] 5. b-FGF: Thermo Fisher Scientific (China) Co., Ltd.

[0060] 6. Pure water

[0061] 2. Prescription:

[0062]

[0063] That is, every 10ml composition consists of the following ingredients

[0064]

[0065] Second, the preparation method:

[0066] Add the above raw materials into a sterile container that can be stirred, and fully stir at 4°C to fully mix the components to achieve a homogeneous state.

[0067] This product involves active ingredients such as growth factors, and the storage effect is best at 4°C.

Embodiment 3

[0068] Example 3: Skin anti-aging composition

[0069] One, raw material: with embodiment 2

[0070] 2. Prescription: Each 10ml composition consists of the following ingredients

[0071]

[0072] Three, preparation method: with embodiment 2.

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PUM

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Abstract

The invention relates to a skin anti-ageing composition, in particular to a skin care product containing kinds of biological factors, and belongs to the fields of cosmetics and biotechnologies. Every10 ml of composition is composed of, by content, 5-40 mg of transparent hyaluronic acid, 50-400 mg of hydrolyzed collagen, 1-5 ml of mesenchymal stem cell secretion, 1-5 ng of EGF, 1-8 ng of b-FGF1 and 10 ml of sufficiently-supplemented purified water. The skin anti-ageing composition has the advantages that a formula is safe and effective, there are no allergic substances and preservative, ageingresistance and wrinkle removal are effective, the premature ageing of the skin is improved greatly, meanwhile, it is avoided that nutrition excess makes the skin burdened, and new skin problems appear, and the skin anti-ageing composition is particularly suitable for allergic skin.

Description

technical field [0001] The invention relates to a skin anti-aging composition, especially a skin care product containing multiple biological factors, and belongs to the fields of cosmetics and biotechnology. Background technique [0002] With age, the content and arrangement, change or decrease of collagen, elastin, hyaluronic acid and glycoprotein that make up the skin. The increase in stress caused by various harmful pollutants and the increase in active oxygen caused by sunlight accelerates degeneration and denaturation, so the skin matrix is ​​destroyed and becomes thinner and thinner. Skin aging symptoms appear. People's skin becomes thinner, wrinkles increase, skin elasticity decreases, skin tone becomes dull, skin problems appear frequently, and age spots, freckles and dark spots also increase. [0003] With the expansion of the cosmetic plastic surgery market, more and more people choose to use laser freckle removal, photon skin rejuvenation and other methods for fa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/99A61K8/73A61K8/65A61K8/64A61Q19/08A61Q19/00A61Q17/04A61Q19/02
CPCA61K8/99A61K8/64A61K8/65A61K8/735A61Q17/04A61Q19/00A61Q19/005A61Q19/02A61Q19/08
Inventor 荆东辉康健
Owner 灏灵赛奥(天津)生物科技有限公司
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