Rhodopseudomonas palustris P-3 as well as screening method and application thereof

Abstract

The invention discloses Rhodopseudomonas palustris P-3. The preservation number of the Rhodopseudomonas palustris P-3 is CCTCC (China Center For Type Culture Collection) NO: M 2020808. According to the strain, photosynthetic bacteria are separated and purified by adopting a double-layer plate coating method and a streaking method, vibrio parahaemolyticus, vibrio vulnificus and vibrio anguillarum are used as indicator bacteria, and the bacteriostatic action of the marine photosynthetic bacteria strain is determined by adopting an oxford cup method. Naphthalene ethylenediamine hydrochloride spectrophotometry and indophenol blue spectrophotometry are adopted to determine the nitrite nitrogen and ammonia nitrogen degradation effects of different strains, and screening is performed to obtain the product. The strain P-3 has the composite functions of resisting vibrio and efficiently degrading nitrite nitrogen and ammonia nitrogen, has a strong inhibition effect on vibrio anguillarum, has thestrongest effect on vibrio anguillarum, and also has a certain nitrite nitrogen and ammonia nitrogen degradation effect. The strain P-3 is cultured in a culture medium containing 50mg / L ammonia nitrogen and nitrite nitrogen for 4 days, the degradation rates are 89.68% and 94.98% respectively, and the strain P-3 has a wide application prospect.

Description

technical field [0001] The present invention relates to a bacterial strain, especially a Rhodopseudomonas palustris P-3. The invention also relates to the screening method and application of the bacterial strain. Background technique [0002] Vibrio is ubiquitous in aquaculture waters and is one of the main pathogenic bacteria in aquaculture. In recent years, with the increase of aquaculture species and the expansion of aquaculture scale, high-density aquaculture has reduced the regulation ability of water areas. The negative impact of vibrio disease and excessive ammonia nitrogen on aquaculture is increasing. Vibrio disease prevention and control in the process of aquaculture And the research on water pollution control is getting more and more attention. [0003] At present, chemical and physical means are mainly used, but physical and chemical means will cause secondary pollution of water bodies, lead to drug resistance of pathogenic bacteria, and break the ecological ba...

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Problems solved by technology

[0003] At present, chemical and physical means are mainly used, but physical and chemical means will cause secondary pollution of water bodies, lead to drug resistance of pathogenic bacteria, and break the ecological balance of water areas. Biological means make up for the shortage of chemical means. This kind of eco-friendly treatment means is becoming more and more The more attention is paid, the more microbial agents are needed in production for the control of aquatic diseases and water pollution

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