Screening method for heterotrophic nitrification-aerobic denitrification bacterial strain

Abstract

The invention discloses a screening method for a heterotrophic nitrification-aerobic denitrification bacterial strain. The method comprises the following steps that sludge is put in a screening culturing medium, screened and purified to obtain a primary sample; the primary sample is firstly subjected to enrichment culturing and then is primarily screened to obtain a pure bacterium, the bacterial strain is pichia jadinii Pichia jadinii MA1, and the preservation number is CCTCCM 2018289; the method of first enrichment culturing and screening afterwards is adopted, the denitrification capacity ofthe bacterial strain can be greatly improved, and the efficiency and effect of purifying waste water are improved; under the circumstance of taking ammonium chloride as the unique nitrogen source, the total nitrogen removal rate of 100 mg / L of initial nitrate reaches 66.9% or above within 24 hours; the primary screening method is adopted, after the pure bacterium is selected, a secondary screening method is adopted to select a pure bacterial strain, the denitrification effect is the best, and therefore the best bacterial strain is obtained.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a screening method for heterotrophic nitrification-aerobic denitrification bacterial strains. Background technique [0002] Under human activities, water resources such as lakes and reservoirs in our country are polluted, and some even cannot be used as water supply resources. Reports in recent years have shown that surface water pollution across the country is serious, and they are all polluted by nitrogen compounds to varying degrees. These nitrogen pollutants are one of the main causes of water pollution and eutrophication in lakes and reservoirs, so the removal of nitrogen from water bodies is of great significance to purify water resources. [0003] Although the traditional denitrification process such as chemical method and physical chemical method can treat wastewater well, there are some defects, such as causing secondary pollution and high cost. Biodegradation is consider...

AI Technical Summary

Problems solved by technology

[0004] In the existing biological denitrification wastewater treatment process, most of the denitrification bacteria are found to remove ammonia nitrogen, but the bacteria are easy to lose due to poor sedimentation, so the reaction time is long and the denitrification efficiency is low; while the mycelium of fungi has good sedimentation performance , easy to stay in the reactor, reducing the loss of bacteria, thereby improving its denitrification efficiency