Preparation method of composite zymoprotein
A composite enzyme and protein technology, applied in the field of composite enzyme protein preparation, can solve the problems of poor stability, low activity and high production cost, and achieve the effects of improved nutritional value, enhanced enzyme-producing ability, and excellent strains
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Embodiment 1
[0018] 1. Aerobic fermentation medium configuration: mix blood meal, leather powder, feather and bran in a weight ratio of 1:2:1:12, and mix the mixture with water in a weight ratio of 1.8:1;
[0019] 2. Anaerobic fermentation medium configuration: mix pomace, rice bran, and sweet potato powder residue in a weight ratio of 3:1:1, and mix the mixture with water in a weight ratio of 6:4;
[0020] 3. Medium sterilization: Put the medium into the sterilizer for high-temperature steam sterilization;
[0021] 4. Strain activation: ① Configure the basic medium according to the aerobic fermentation strain and the anaerobic fermentation strain; ② Packing: divide the prepared medium into the triangular flask to be inoculated, and plug it with a cotton plug; ③ Sterilization: Sterilize at 0.1MPa for 15 minutes, then let it naturally depressurize to normal pressure, cool, and wait for inoculation; ④ Inoculation: Wait for the medium to cool to 40°C for inoculation, and the inoculum volume i...
Embodiment 2
[0030] 1. Aerobic fermentation medium configuration: mix blood meal, leather powder, feather and bran at a ratio of 1:2:1:12, and mix the mixture with water at a ratio of 1.8:1;
[0031] 2. Anaerobic fermentation medium configuration: mix pomace, rice bran, and sweet potato powder residue at a ratio of 3:1:1, and mix the mixture with water at a ratio of 6:4;
[0032] 3. Medium sterilization: Put the medium into the sterilizer for high-temperature steam sterilization;
[0033] 4. Strain activation: ① Configure the basic medium according to the requirements of each strain; ② Packing: divide the prepared medium into the triangular flask to be inoculated, and plug it with a cotton plug; ③ Sterilization: at 0.1MPa Sterilize for 15 minutes, then let it naturally reduce the pressure to normal pressure, cool down, and wait for inoculation; ④ Inoculation: Wait for the medium to cool to 40°C to inoculate, and the inoculation amount is 0.5%; ⑤ Culture: Put the inoculated test tube into a...
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